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US4292041A
Expired / Lapsed
US
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Surfactant assay
Assignee: Merck and Co Inc
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Filing: 1979-11-02
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Granted: 1981-09-29
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Expires: 1999-11-02
Abstract
Abstract Thin layer chromatography is used to separate various surfactants. The support medium is silica gel on a glass or acrylamide plate. The developing solvent is chloroform/methanol/water. After drying, the surfactants are detected at extremely low levels with iodine vapor, rhodamine 6G in methanol or phosphomolybdic acid in ethanol/ether, or radiolabelled surfactant. A method is given for the separation of surfactants from proteins or glycoproteins or lipoproteins or polysaccharides, or lipopolysaccharides.
Inventors
- 👤 W. Wardle Fullerton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 08:14
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/90; G01N30/94; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
118 words
A chromatographic method for the separation, detection and quantitation of an anionic, nonionic or cationic surfactant alone or in a surfactant-containing protein, glycoprotein, lipoprotein, polysaccharide, or lipopolysaccharide at levels below about 5 μg 1 mg comprising extracting the surfactant or surfactant-containing protein, glycoprotein, lipoprotein, polysaccharide, or lipopolysaccharide with a solvent mixture comprising a haloalkane, a water-miscible alkanol and water, subjecting the extract containing the surfactant to thin layer chromatography wherein the support medium is a silica or alumina gel and the developing solvent is a mixture comprising a haloalkane, a water-miscible alkanol and water, removing the developing solvent, and detecting the surfactant sequentially with (a) iodine vapor, (b) rhodamine 6G and (c) phosphomolybdic acid, or with radiolabeled surfactant.
Claim 2
Dependent Claim
118 words
A chromatographic method for the separation, detection and quantitation of an anionic, nonionic or cationic surfactant alone or in a surfactant-containing protein, glycoprotein, lipoprotein, polysaccharide, or lipopolysaccharide at levels below about 5 μg 1 mg comprising extracting the surfactant or surfactant-containing protein, glycoprotein, lipoprotein, polysaccharide, or lipopolysaccharide with a solvent mixture comprising a haloalkane, a water-miscible alkanol and water, subjecting the extract containing the surfactant to thin layer chromatography wherein the support medium is a silica or alumina gel and the developing solvent is a mixture comprising a haloalkane, a water-miscible alkanol and water, removing the developing solvent, and detecting the surfactant sequentially with (a) iodine vapor, (b) rhodamine 6G and (c) phosphomolybdic acid, or with radiolabeled surfactant.
Claim 3
Dependent Claim
23 words
A method according to claim 1 wherein the detection is effected sequentially with (a) iodine vapor, (b) rhodamine 6G and (c) phosphomolybdic acid.
Claim 4
Dependent Claim
14 words
A method according to claim 1 wherein the detection is effected with radiolabeled surfactant.
Claim 5
Dependent Claim
15 words
A method according to claim 1 wherein the rhodamine 6G is employed in alcohol solution.
Claim 6
Dependent Claim
11 words
A method according to claim 4 wherein the alcohol is methanol.
Claim 7
Dependent Claim
14 words
A method according to claim 1 wherein the phosphomolybdic acid is employed in ethanol/ether.
Claim 8
Dependent Claim
11 words
A method according to claim 1 wherein the haloalkane is chloroform.
Claim 9
Dependent Claim
12 words
A method according to claim 1 wherein the water-miscible alkanol is methanol.
Claim 10
Dependent Claim
11 words
A method according to claim 8 wherein the haloalkane is chloroform.
Claim 11
Dependent Claim
17 words
A method according to claim 1 wherein the extracting and developing solvents comprise chloroform, methanol and water.
References Cited (Backward Citations)
US4064041A
Cited
US4129560A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-28 | 1985-03-30 | 1985-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-28 | 1989-03-30 | 1989-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-28 | 1993-03-30 | 1993-09-29 | $7700.00 | Window Closed / Lapsed |