Patent US4286062A successfully refreshed from Google Patents.
US4286062A
Expired / Lapsed
US
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Process for producing an enzyme preparation to tenderize meat products
Assignee: Individual
•
Filing: 1979-11-14
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Granted: 1981-08-25
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Expires: 1999-11-14
Abstract
Abstract An enzyme preparation for tenderizing meat products is produced from endocrine-enzyme raw stock. The testes of slaughtered cattle are comminuted and mixed with acidulated water to obtain a mixture having the pH value within the range 4.5 to 4.7, followed by isolation of an aqueous solution from said mixture, the solution containing hyaluronidase enzyme. An albumin-containing substance is then added, the ratio of albumin-containing substance to the testes being between 0.04-1.6:10-15, to obtain a suspension containing the end product. The suspension is concentrated by evaporation in vacuo at a maximum temperature of 25° C. to form a concentrate of the end product, which is then dried.
Inventors
- 👤 Leonid I. Stekolnikov
- 👤 Boris A. Sevastyanov
- 👤 Gennady G. Shilov
- 👤 Anatoly A. Belousov
- 👤 Nikolai D. Mamonov
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 18:25
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; A; A23; A23L; A23L13/00; A23L13/40; A23L13/48; A23L13/70; A23L13/72; A23L13/74; C12N9/96; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
120 words
Method of producing an enzyme meat tenderizing agent, which comprises comminuting the testes of slaughtered animals, extracting the thus-comminuted testes with acidudated water of a pH and in an amount sufficient to obtain a mixture having a pH value of 4.5-4.7, separating an aqueous solution from the thus obtained mixture, said aqueous solution containing hyaluronidase, adding an albumin-containing substance to said aqueous solution in an amount such that the ratio of said albumin-containing substance to the testes of the slaughtered animals is between about 0.04-1.6:10-15, thus forming a suspension, subjecting the thus-formed suspension to concentration by evaporation at a maximum temperature of 25° C., thus obtaining a concentrate of the enzyme meat tenderizing agent, and drying the thus obtained concentrate.
Claim 2
Dependent Claim
120 words
Method of producing an enzyme meat tenderizing agent, which comprises comminuting the testes of slaughtered animals, extracting the thus-comminuted testes with acidudated water of a pH and in an amount sufficient to obtain a mixture having a pH value of 4.5-4.7, separating an aqueous solution from the thus obtained mixture, said aqueous solution containing hyaluronidase, adding an albumin-containing substance to said aqueous solution in an amount such that the ratio of said albumin-containing substance to the testes of the slaughtered animals is between about 0.04-1.6:10-15, thus forming a suspension, subjecting the thus-formed suspension to concentration by evaporation at a maximum temperature of 25° C., thus obtaining a concentrate of the enzyme meat tenderizing agent, and drying the thus obtained concentrate.
Claim 3
Dependent Claim
16 words
The process of claim 1, wherein said concentrate is subjected to filtration prior to being dried.
Claim 4
Dependent Claim
19 words
The process of claim 1, wherein said concentrate is subjected to separation from suspended material prior to being dried.
Claim 5
Dependent Claim
25 words
The process of claim 1, wherein blood serum is used as the albumin-containing substance, the ratio of blood serum to the testes being between 0.8-1.6:10-15.
Claim 6
Dependent Claim
31 words
The process of claim 1, wherein a clear aqueous solution of food albumin is used as the albumin-containing substance, the ratio of said food albumin to the testes being between 0.04-0.08:10-15.
Claim 7
Dependent Claim
22 words
The process of claim 1, wherein the concentrate is freeze-dried, with the layer of said concentrate being 2 to 12 mm deep.
References Cited (Backward Citations)
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US2488565A
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US2676139A
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US2806815A
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US3728223A
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US3945889A
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US4195097A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-24 | 1985-02-23 | 1985-08-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-24 | 1989-02-23 | 1989-08-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-24 | 1993-02-23 | 1993-08-25 | $7700.00 | Window Closed / Lapsed |