Hyaluronidase

Claim 2

A PROCESS FOR OBTAINING A HYALURONIDASE PRODUCT COMPRISING, DISSOLVING CRUDE HYALURONIDASE DERIVED FROM ANIMAL TISSUE IN A SUBSTANTIALLY AQUEOUS MEDIUM TO FORM A SOLUTION OF APPROXIMATELY 1-5% BY WEIGHT, ADJUSTING THE PH OF THE SOLUTION WITHIN THE RANGE OF SUBSTANTIALLY PH 6 TO NOT MORE THAN PH 9, ALSO ADJUSTING THE IONIC STRENGTH TO BELOW 0.3, LOWERING THE TEMPERATURE TO BELOW 5* C. AND THEN ADDDING SUFFICIENT WATER-SOLUBLE, ORGANIC PRECIPITATING AGENT TO OBTAIN A CONCENTRATION OF ABOUT 20-40% BY VOLUME, THEREBY PRECIPITATING THE HYALURONIDASE PRODUCT FROM SAID SOLUTION.

Claim 3

IN A PROCESS FOR OBTAINING A HIGHLY ACTIVE HYALURONIDASE PRODUCT WHEREIN ANIMAL TISSUE CONTAINING HYALURONIDASE IS TREATED WITH A SUBSTANTIALLY AQUEOUS MEDIUM IN ORDER TO TO EXTRACT THEREFROM A CRUDE HYALURONIDASE FRACTION AND WHICH IS THEN CONTACTED WITH WATER-SOLUBLE INORGANIC SALTS AT DIFFERENT CONCENTRATIONS TO FIRST PRECIPITATE A RELATIVELY INERT FRACTION AND THEN A RELATIVELY ACTIVE FRACTION, AND WHEREIN THE RELATIVELY ACTIVE FRACTION IS SELECTIVELY REMOVED AND DIALYZED TO SEPARATE OUT EXCESS SALTS THEREFROM, THE IMPROVEMENT COMPRISING DISSOLVING THE RELATIVELY ACTIVE FRACTION IN AN AQUEOUS MEDIUM TO A CONCENTRATION OF ABOUT 1 TO 5% BY WEIGHT, ADJUSTING THE PH OF THE SOLUTION TO WITHIN THE RANGE OF SUBSTANTIALLY PH 6 TO 9 AND THE IONIC STRENGTH TO ABOUT 0.15 TO ABOUT 0.2, ADJUSTING THE TEMPERATURE OF THE SOLUTION TO APPROXIMATELY 0* C., ADDING A WATER-SOLUBLE, ORGANIC, PROTEIN-PRECIPITATING AGENT TO SAID SOLUTION IN AN AMOUNT TO OBTAIN A CONCENTRATION OF APPROXIMATELY 15 TO 20% BY VOLUME OF SIAD PROTEIN-PRECIPITATING AGENT, DISCARDING THE PRECIPITATE OBTAINED, AND THEN PRECIPITATING AN ACTIVE HYALURONIDASE PRODUCT BY ADDING SUFFICIENTLY MORE WATER-SLUBLE, ORGANIC PRECIPITATING AGENT TO OBTAIN A SUBSTANTIALLY HIGHER CONCENTRATION BUT NOT EXCEEDING ABOUT 40% BY VOLUME OF AGENT IN SAID SOLUTION, SAID SOLUTION BEING MAINTAINED BELOW ABOUT 0* C. DURING SAID ORGANIC AGENT PRECIPITATIONS.

Claim 4

IN A PROCESS FOR OBTAINING A HIGHLY ACTIVE HYALURONIDASE PRODUCT WHEREIN ANIMAL TISSUE CONTAINING HYALURONIDASE IS TREATED WITH A SUBSTANTIALLY AQUEOUS MEDIUM IN ORDER TO TO EXTRACT THEREFROM A CRUDE HYALURONIDASE FRACTION AND WHICH IS THEN CONTACTED WITH WATER-SOLUBLE INORGANIC SALTS AT DIFFERENT CONCENTRATIONS TO FIRST PRECIPITATE A RELATIVELY INERT FRACTION AND THEN A RELATIVELY ACTIVE FRACTION, AND WHEREIN THE RELATIVELY ACTIVE FRACTION IS SELECTIVELY REMOVED AND DIALYZED TO SEPARATE OUT EXCESS SALTS THEREFROM, THE IMPROVEMENT COMPRISING DISSOLVING THE RELATIVELY ACTIVE FRACTION IN AN AQUEOUS MEDIUM TO A CONCENTRATION OF ABOUT 1 TO 5% BY WEIGHT, ADJUSTING THE PH OF THE SOLUTION TO WITHIN THE RANGE OF SUBSTANTIALLY PH 6 TO 9 AND THE IONIC STRENGTH TO ABOUT 0.15 TO ABOUT 0.2, ADJUSTING THE TEMPERATURE OF THE SOLUTION TO APPROXIMATELY 0* C., ADDING A WATER-SOLUBLE, ORGANIC, PROTEIN-PRECIPITATING AGENT TO SAID SOLUTION IN AN AMOUNT TO OBTAIN A CONCENTRATION OF APPROXIMATELY 15 TO 20% BY VOLUME OF SIAD PROTEIN-PRECIPITATING AGENT, DISCARDING THE PRECIPITATE OBTAINED, AND THEN PRECIPITATING AN ACTIVE HYALURONIDASE PRODUCT BY ADDING SUFFICIENTLY MORE WATER-SLUBLE, ORGANIC PRECIPITATING AGENT TO OBTAIN A SUBSTANTIALLY HIGHER CONCENTRATION BUT NOT EXCEEDING ABOUT 40% BY VOLUME OF AGENT IN SAID SOLUTION, SAID SOLUTION BEING MAINTAINED BELOW ABOUT 0* C. DURING SAID ORGANIC AGENT PRECIPITATIONS.