Patent US4281062A successfully refreshed from Google Patents.
US4281062A
Expired / Lapsed
US
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Test for the identification of glucose and for the determination of glucose
Assignee: Teva GmbH
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Filing: 1979-07-23
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Granted: 1981-07-28
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Expires: 1999-07-23
Abstract
Abstract Glucose identification and determination test. A test strip contains a metering zone, a reaction zone with glucose oxidase, and an indicator zone having at least one indicator signal zone of peroxidase and indicator applied in the form of stripes alternating with untreated intermediate spaces. The metering zone may be impregnated with a colorant, oxidizing, precipitating or buffering agent. The glucose oxidase is applied in an acid solution below the isoelectric point to prevent substrate inhibition by means of test fluid diffusion and may be in the form of an immobilized suspension. Together with a flow-inducing agent, the test strip allows a reaction not influenced by the time elapsed after sampling. Intermediate zones located between the reaction zone and the metering zone and/or the identification zone may be prepared with buffering substances. The test strip may be located within a capillary tube of 1-3 mm diameter and 40-120 length made of glass or plastic.
Inventors
- 👤 Karl-Heinz Kallis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:44
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
118 words
Test for the identification of glucose and for the determination of glucose, comprising locating, in given instances within a capillary tube marked at both its ends, a test strip which, conjointly with a flow-inducing agent, will allow a reaction not influenced by the time elapsed after sampling, the test strip containing a metering zone, a reaction zone with glucose oxidase applied in an acid solution below the isoelectric point and affixed to it to prevent a substrate inhibition by means of the diffusion of the test fluid taking place within it, and an indicator zone having at least one indicator signal zone of peroxidase and indicator applied in the form of stripes and alternating with untreated intermediate spaces.
Claim 2
Dependent Claim
118 words
Test for the identification of glucose and for the determination of glucose, comprising locating, in given instances within a capillary tube marked at both its ends, a test strip which, conjointly with a flow-inducing agent, will allow a reaction not influenced by the time elapsed after sampling, the test strip containing a metering zone, a reaction zone with glucose oxidase applied in an acid solution below the isoelectric point and affixed to it to prevent a substrate inhibition by means of the diffusion of the test fluid taking place within it, and an indicator zone having at least one indicator signal zone of peroxidase and indicator applied in the form of stripes and alternating with untreated intermediate spaces.
Claim 3
Dependent Claim
20 words
Test as per claim 1, characterized by the metering zone being impregnated with a colorant, oxidizing, precipitating or buffering agent.
Claim 4
Dependent Claim
23 words
Test as per claim 2, characterized by the intermediate space being located within the identification zone containing a fixing agent for the colorant.
Claim 5
Dependent Claim
20 words
Test as per claim 1, characterized by the reaction zone containing as a suspension glucose oxidase which has been immobilized.
Claim 6
Dependent Claim
19 words
Test as per claim 1, characterized by an intermediate zone located between the metering zone and the reaction zone.
Claim 7
Dependent Claim
21 words
Test as per claim 5, wherein said intermediate zone is different in color from said metering zone and said reaction zone.
Claim 8
Dependent Claim
18 words
Test as per claim 5, wherein said intermediate zone is saturated with reagents for buffering and masking purposes.
Claim 9
Dependent Claim
19 words
Test as per claim 1, characterized by locating between the reaction zone and the identification zone an intermediate zone.
Claim 10
Dependent Claim
13 words
Test as per claim 8, wherein said intermediate zone further comprises buffering substances.
Claim 11
Dependent Claim
18 words
Test as per claims 5, 6, 7 or 8, characterized by the intermediate zones being of different widths.
Claim 12
Dependent Claim
33 words
Test as per claim 1, characterized by the capillary tube having an internal diameter of 1-3 mm and a length of 40-120 mm and being of glass or a plastic of glass-like transparency.
Claim 13
Dependent Claim
12 words
Test as per claim 11, wherein said internal diameter is 2.5 mm.
Claim 14
Dependent Claim
11 words
Test as per claim 11, wherein said length is 60 mm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-27 | 1985-01-26 | 1985-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-27 | 1989-01-26 | 1989-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-27 | 1993-01-26 | 1993-07-28 | $7700.00 | Window Closed / Lapsed |