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Patent US4277564A successfully refreshed from Google Patents.
US4277564A
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Preparing entomocidal products with oligosporogenic mutants of bacillus thuringiensis
Assignee: United States Department of Agriculture
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Filing: 1980-01-09
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Granted: 1981-07-07
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Expires: 2000-01-09
Abstract
Abstract Six oligosporogenic mutant strains obtained from Bacillus thuringiensis subsp. kurstaki yield parasporal products substantially free of interfering spores when cultivated under normal sporulation conditions. These parasporal products have utility in the biological control of pest insects.
Inventors
- 👤 Donovan E. Johnson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:00
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/32; C07K14/325; A; A01; A01N; A01N63/00; A01N63/20; A01N63/22; A01N63/23; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
84 words
A method of producing a Bacillus thuringiensis parasporal product substantially free of contaminating spores comprising culturing an oligosporogenic mutant of Bacillus thuringiensis subspecies kurstaki having the identifying characteristics of a strain selected from the group consisting of NRRL B-4453, NRRL B-4454, NRRL B-4455, NRRL B-4456, NRRL B-4457, and NRRL B-4458 on a nutritionally sufficient medium whereby the vegetative cells of said mutant culture produce spores and parasporal crystals in a spore:crystal ratio of less than about 1:800, and recovering said substantially spore-free parasporal product.
Claim 2
Independent Claim
84 words
A method of producing a Bacillus thuringiensis parasporal product substantially free of contaminating spores comprising culturing an oligosporogenic mutant of Bacillus thuringiensis subspecies kurstaki having the identifying characteristics of a strain selected from the group consisting of NRRL B-4453, NRRL B-4454, NRRL B-4455, NRRL B-4456, NRRL B-4457, and NRRL B-4458 on a nutritionally sufficient medium whereby the vegetative cells of said mutant culture produce spores and parasporal crystals in a spore:crystal ratio of less than about 1:800, and recovering said substantially spore-free parasporal product.
Claim 3
Dependent Claim
16 words
The method as described in claim 1 wherein the spore:crystal ratio is less than about 1:1600.
Claim 4
Dependent Claim
17 words
The method as described in claim 1 wherein said strain has the identifying characteristics of NRRL B-4454.
Claim 5
Dependent Claim
17 words
The method as described in claim 1 wherein said strain has the identifying characteristics of NRRI B-4457.
Claim 6
Dependent Claim
17 words
The method as described in claim 1 wherein said strain has the identifying characteristics of NRRL B-4458.
Claim 7
Dependent Claim
64 words
A biologically pure culture of an oligosporogenic mutant of Bacillus thuringiensis subspecies kurstaki having the identifying characteristics of a strain selected from the group consisting of NRRL B-4453, NRRL B-4454, NRRL B-4455, NRRL B-4456, NRRL B-4457, and NRRL B-4458 wherein said culture is characterized by the ability to produce a parasporal product substantially free of contaminating spores upon fermentation in a nutritionally sufficient medium.
Claim 8
Dependent Claim
64 words
A biologically pure culture of an oligosporogenic mutant of Bacillus thuringiensis subspecies kurstaki having the identifying characteristics of a strain selected from the group consisting of NRRL B-4453, NRRL B-4454, NRRL B-4455, NRRL B-4456, NRRL B-4457, and NRRL B-4458 wherein said culture is characterized by the ability to produce a parasporal product substantially free of contaminating spores upon fermentation in a nutritionally sufficient medium.
Claim 9
Dependent Claim
19 words
A biologically pure culture as described in claim 6 wherein said strain has the identifying characteristics of NRRL B-4454.
Claim 10
Dependent Claim
19 words
A biologically pure culture as described in claim 6 wherein said strain has the identifying characteristics of NRRL B-4457.
Claim 11
Dependent Claim
19 words
A biologically pure culture as described in claim 6 wherein said strain has the identifying characteristics of NRRL B-4458.
References Cited (Backward Citations)
US3578383A
Cited
US4133716A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-06 | 1985-01-05 | 1985-07-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-06 | 1989-01-05 | 1989-07-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-06 | 1993-01-05 | 1993-07-07 | $7700.00 | Window Closed / Lapsed |