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US4273872A
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Method for the microbiological conversion of steroids
Assignee: Anic SpA
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Filing: 1979-11-13
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Granted: 1981-06-16
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Expires: 1999-11-13
Abstract
Abstract A method is disclosed for the microbiological transformation of steroids, especially cholesterol, by employing culture media which contain hydrocarbons rather than carbohydrates. Paraffinic hydrocarbons, particularly normal paraffins from 15 to 20 carbon atoms, are used as the only carbon and energy sources. SP2T (NRRL B-11, 112), SP3T bis (NRRL B-11, 113), SP5T (NRRL B-11, 114), SP7T/1 (NRRL B-11, 115), SP742XXA (NRRL B-11, 116), SP731RD (NRRL B-11, 117), and SP734D (NRRL B-11, 118) are bacterial strains which can be used to this purpose.
Inventors
- 👤 Pasquale Zaffaroni
- 👤 Vincenza Vitobello
- 👤 Anna M. Gamalerio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 12:30
CPC Classifications
C; C07; C07J; C07J9/00; C07J1/00; C07J1/0003; C07J1/0011; C12; C12P; C12P33/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
60 words
A method for the mircobiological preparation of Δ-4-cholestene-3-one from cholesterol which comprises adding cholesterol and a mixture of n-parafins to a culture of an organism selected from the group cosisting of SP2T, SP3T bis, SP5T, and SP7T/1; incubating the culture for a sufficient time to produce Δ-4-cholestene-3-one; and thereafter extracting the culture with a water immiscible solvent to obtain Δ-4-cholestern-3-one.
Claim 2
Independent Claim
60 words
A method for the mircobiological preparation of Δ-4-cholestene-3-one from cholesterol which comprises adding cholesterol and a mixture of n-parafins to a culture of an organism selected from the group cosisting of SP2T, SP3T bis, SP5T, and SP7T/1; incubating the culture for a sufficient time to produce Δ-4-cholestene-3-one; and thereafter extracting the culture with a water immiscible solvent to obtain Δ-4-cholestern-3-one.
Claim 3
Dependent Claim
12 words
A method as defined in claim 1 wherein the organism is SP2T.
Claim 4
Dependent Claim
13 words
A method as defined in claim 1 wherein the organism is SP3T bis.
Claim 5
Dependent Claim
12 words
A method as defined in claim 1 wherein the organism is SP5T.
Claim 6
Dependent Claim
12 words
A method as defined in claim 1 wherein the organism is SP7T/1.
Claim 7
Dependent Claim
16 words
A method as defined in claim 1 wherein the n-parafins are C 15 -C 20 n-parafins.
Claim 8
Independent Claim
50 words
A method for the production of Δ 1,4-androstadiene-3,17-dione from cholesterol which comprises adding cholesterol and a mixture of n-parafins to a culture of the bacterial strain SP7T/1 incubating the culture for a sufficient period of time to form Δ 1,4-androstadiene-3,17-dione and thereafter extracting the culture with a water immiscible solvent.
Claim 9
Independent Claim
50 words
A method for the production of Δ 1,4-androstadiene-3,17-dione from cholesterol which comprises adding cholesterol and a mixture of n-parafins to a culture of the bacterial strain SP7T/1 incubating the culture for a sufficient period of time to form Δ 1,4-androstadiene-3,17-dione and thereafter extracting the culture with a water immiscible solvent.
Claim 10
Independent Claim
65 words
A method for the production of Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione from progesterone which comprises adding progesterone and a mixture of n-parafins to a culture of SP 731 RO or SP 742 XXA and incubating the culture for a sufficient time to produce Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione; and thereafter extracting the culture with a water immiscible solvent to obtain Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione.
Claim 11
Independent Claim
65 words
A method for the production of Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione from progesterone which comprises adding progesterone and a mixture of n-parafins to a culture of SP 731 RO or SP 742 XXA and incubating the culture for a sufficient time to produce Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione; and thereafter extracting the culture with a water immiscible solvent to obtain Δ 4-androstene-3,17-dione and Δ 1,4-androstadiene-3,17-dione.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |