Patent US4273868A successfully refreshed from Google Patents.
US4273868A
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Color stable glucose test
Assignee: Bayer Corp
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Filing: 1979-02-23
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Granted: 1981-06-16
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Expires: 1999-02-23
Abstract
Abstract Test means, such as a composition, a test device, method of making the test device and process for determining glucose in a body fluid sample are disclosed. More particularly, the contemplated test means comprises glucose oxidase, a peroxidatively active substance, such as peroxidase, and a 3,3',5,5'-tetraalkylbenzidine indicator in an amount sufficient rapidly to produce upon contact of the test means with a predetermined amount of a glucose containing sample, a stable colored reaction product. The preferred benzidine indicator is 3,3',5,5'-tetramethylbenzidine.
Inventors
- 👤 Bert Walter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
58 words
A composition for the determination of glucose in a body fluid sample which comprises glucose oxidase, peroxidase, a buffer effective to maintain a pH of from about 4 to about 7.5, a stabilizing agent and 3,3',5,5'tetramethylbenzidine, wherein the 3,3',5,5'tetramethylbenzidine is present in a concentration of at least about 2.6 millimoles per thousand International Units of glucose oxidase activity.
Claim 2
Dependent Claim
58 words
A composition for the determination of glucose in a body fluid sample which comprises glucose oxidase, peroxidase, a buffer effective to maintain a pH of from about 4 to about 7.5, a stabilizing agent and 3,3',5,5'tetramethylbenzidine, wherein the 3,3',5,5'tetramethylbenzidine is present in a concentration of at least about 2.6 millimoles per thousand International Units of glucose oxidase activity.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |