US3853470A
Expired / Lapsed
US
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Reagent and method for glucose determination
Assignee: Medico Electronic Inc
•
Filing: 1972-08-30
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Granted: 1974-12-10
•
Expires: 1992-08-30
Abstract
Abstract A reagent and method for the fast (under 5 minutes) determination of glucose in biological fluids by the peroxidase catalyzed oxidation of sulfonated phenyl amines or sulfonated benzidines by hydrogen peroxide formed from the action of glucose oxidase on glucose.
Inventors
- 👤 L Morin
- 👤 J Prox
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:49
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
70 words
A REAGENT FOR GLUCOSE DETERMINATION IN BIOLOGICAL FLUIDS CONSISTING OF GLUCOSE OXIDASE, PEROXIDASE, A MEMBER SELECTED FROM HE GROUP CONSISTING OF A SULFONATED PHENYLAMINE REDOX INDICATORR AND A SULFONATED BENZIDINE REDOX INDICATOR, WATER AND A BUFFER, THE PH BEING BETWEEN 4 AND 5.9 AND THE RATIO OF GLUCOSE OXIDAZE BASED ON THE EU UNITS THEREOF PRESENT, TO PEROXIDASE, BASED ON THE PURPUROGALLIN UNITS THEREOF PRESENT, BEING BETWEEN 250:3 AND 50:1.
Claim 2
Dependent Claim
70 words
A REAGENT FOR GLUCOSE DETERMINATION IN BIOLOGICAL FLUIDS CONSISTING OF GLUCOSE OXIDASE, PEROXIDASE, A MEMBER SELECTED FROM HE GROUP CONSISTING OF A SULFONATED PHENYLAMINE REDOX INDICATORR AND A SULFONATED BENZIDINE REDOX INDICATOR, WATER AND A BUFFER, THE PH BEING BETWEEN 4 AND 5.9 AND THE RATIO OF GLUCOSE OXIDAZE BASED ON THE EU UNITS THEREOF PRESENT, TO PEROXIDASE, BASED ON THE PURPUROGALLIN UNITS THEREOF PRESENT, BEING BETWEEN 250:3 AND 50:1.
Claim 3
Dependent Claim
13 words
A reagent according to claim 1 wherein the sulfonated redox indicator is diphenylaminesulfonate.
Claim 4
Dependent Claim
13 words
A reagent according to claim 1 wherein the sulfonated redox indicator is diphenylbenzidinesulfonate.
Claim 5
Dependent Claim
19 words
A reagent according to claim 1 wherein the sulfonated redox indicator is Calcocid Blue 2 G (Color Index 42090).
Claim 6
Dependent Claim
22 words
A reagent according to claim 1 wherein the sulfonated redox indicator is present at a level of 30 to 900 mg percent.
Claim 7
Dependent Claim
14 words
A reagent according to claim 1 wherein the buffer is a sodium citrate buffer.
Claim 8
Dependent Claim
12 words
A reagent according to claim 1 wherein the pH is about 5.5.
Claim 9
Dependent Claim
53 words
A reagent according to claim 1 wherein the reagent contains 5,000 to 25,000 EU units of glucose oxidase, 60 to 400 Purpurogallin units, and one liter of solution made up of 0.3 to 3 grams of one of the redox indicators, 0.01 to 2 M of sodium citrate buffer and the remainder water.
Claim 10
Dependent Claim
68 words
A method for determining glucose in biological fluids which comprises mixing a small sample of biological fluids with the reagent of claim 1, the ratio of the biological fluid to the reagent being between 1:100 and 1:25, incubating the mixture between about room temperature and about 40*C from about one half minute to about ten minutes, and determining the concentration of glucose by means of measuring the abosrbance.
Claim 11
Dependent Claim
19 words
A method according to claim 9 wherein the absorbance is measured at a point between 410 and 740 nm.
Claim 12
Dependent Claim
13 words
A method according to claim 9 wherein the sulfonated redox indicator is diphenylaminesulfonate.
Claim 13
Dependent Claim
13 words
A method according to claim 9 wherein the sulfonated redox indicator is diphenylbenzidinesulfonate.
Claim 14
Dependent Claim
19 words
A method according to claim 9 wherein the sulfonated redox indicator is Calcocid Blue 2 G (Color Index 42090).
Claim 15
Dependent Claim
22 words
A method according to claim 9 wherein the sulfonated redox indicator is present at a level of 30 to 900 mg percent.
Claim 16
Dependent Claim
14 words
A method according to claim 9 wherein the buffer is a sodium citrate buffer.
Claim 17
Dependent Claim
12 words
A method according to claim 9 wherein the pH is about 5.5.
Claim 18
Dependent Claim
77 words
A reagent for glucose determination in biological fluids consisting of glucose oxidase, peroxidase, a member selected from the group consisting of a sulfonated phenylamine redox indicator and a sulfonated benzidine redox indicator, and a buffer, the pH being between 4 and 5.9 when said reagent is admixed with sufficient water, and the ratio of glucose oxidase based on the EU units thereof present, to peroxidase, based on the purpurogallin units thereof present, being between 250:3 and 50:
Claim 19
Dependent Claim
77 words
A reagent for glucose determination in biological fluids consisting of glucose oxidase, peroxidase, a member selected from the group consisting of a sulfonated phenylamine redox indicator and a sulfonated benzidine redox indicator, and a buffer, the pH being between 4 and 5.9 when said reagent is admixed with sufficient water, and the ratio of glucose oxidase based on the EU units thereof present, to peroxidase, based on the purpurogallin units thereof present, being between 250:3 and 50:
Claim 20
Dependent Claim
84 words
A method for determining glucose in biological fluids which comprises admixing the reagent of claim 17 with sufficient water to achieve a pH between 4 and 5.9, mixing a small sample of biological fluids with the resultant reagent, the ratio of the biological fluid to the resultant reagent being between 1:100 and 1:25, incubating the mixture between about room temperature and about 40*C. from about one half minute to about ten minutes, and determining the concentration of glucose by means of measuring the absorbance.
References Cited (Backward Citations)
US2848308A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-09 | 1978-06-10 | 1978-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-09 | 1982-06-10 | 1982-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-09 | 1986-06-10 | 1986-12-10 | $7700.00 | Window Closed / Lapsed |