US4264589A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
Assignee: Individual
•
Filing: 1979-07-17
•
Granted: 1981-04-28
•
Expires: 1999-07-17
Abstract
Abstract This invention relates to a method of separating the active α 1 -acid glycoprotein fraction, a co-factor in the lipoprotein lipase reaction, from urine of nephrotic animals and humans as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; and (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize. This active α 1 -acid glycoprotein fraction is utilized in effective amounts in nephrotic animals to reverse the defect in triglyceride removal caused by the loss of plasma constituents in urine.
Inventors
- 👤 James M. Felts
- 👤 Ilona Staprans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:03
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/22; C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/473; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
105 words
A method of separating and utilizing the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 0 and lyophilize; wherein the utilization of active α 1 -acid glycoprotein containing glycosaminoglycan is made in effective amounts in nephrotic animals to reverse the effect of the loss of plasma constituents in urine.
Claim 2
Independent Claim
105 words
A method of separating and utilizing the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 0 and lyophilize; wherein the utilization of active α 1 -acid glycoprotein containing glycosaminoglycan is made in effective amounts in nephrotic animals to reverse the effect of the loss of plasma constituents in urine.
Claim 3
Independent Claim
103 words
A method of separating and utilizing the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize; wherein the utilization of active α 1 -acid glycoprotein containing glycosaminoglycan is made in effective amounts in nephrotic rats to reduce the increased amount of triglyceride in the blood.
Claim 4
Independent Claim
103 words
A method of separating and utilizing the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize; wherein the utilization of active α 1 -acid glycoprotein containing glycosaminoglycan is made in effective amounts in nephrotic rats to reduce the increased amount of triglyceride in the blood.
References Cited (Backward Citations)
US2001255A
Cited
US3759793A
Cited
US3703591A
Cited
US3862009A
Cited
US3901763A
Cited
US3898130A
Cited
US4056442A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-27 | 1984-10-27 | 1985-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-27 | 1988-10-27 | 1989-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-27 | 1992-10-27 | 1993-04-28 | $7700.00 | Window Closed / Lapsed |