US4256732A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modified grass pollen antigens
Assignee: Research Corp
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Filing: 1979-09-25
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Granted: 1981-03-17
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Expires: 1999-09-25
Abstract
Abstract Oxidative rearrangement and cleavage of hydroxy aromatic rings of quercitin moiety of Timothy grass pollen antigen or antigenic fragments gives rise to a modified antigen having no antigenic properties but retaining capability of activating T-cells for regulation of immune response. The invention includes the modified antigens, the method of oxidative cleavage, immunotherapeutic methods for alleviating allergic responses to the Timothy grass pollen antigens and immunotherapeutic compositions therefor.
Inventors
- 👤 Arthur Malley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:03
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35; A61K39/36
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
124 words
An immunotherapeutic method for treating grass pollen allergies comprising administering to an individual an amount of a modified grass pollen antigen sufficient to induce suppressor T-cells that will result in a suppression of allergic antibodies to grass pollen antigen, said modified antigen being the product of a method comprising subjecting a quercitin moiety containing grass pollen antigen or fragment thereof having the structure: ##STR4## wherein: G represents glucose, and T represents threonine or a poly-peptide linked to said structure through a threonine molecule; to oxidative conditions such that the said dihydroxy aromatic rings undergo rearrangement and ring scission without substantially modifying said threonine or polypeptide to yield a product substantially devoid of antigenic properties but capable of activating T-cells and inducing T s cells.
Claim 2
Dependent Claim
124 words
An immunotherapeutic method for treating grass pollen allergies comprising administering to an individual an amount of a modified grass pollen antigen sufficient to induce suppressor T-cells that will result in a suppression of allergic antibodies to grass pollen antigen, said modified antigen being the product of a method comprising subjecting a quercitin moiety containing grass pollen antigen or fragment thereof having the structure: ##STR4## wherein: G represents glucose, and T represents threonine or a poly-peptide linked to said structure through a threonine molecule; to oxidative conditions such that the said dihydroxy aromatic rings undergo rearrangement and ring scission without substantially modifying said threonine or polypeptide to yield a product substantially devoid of antigenic properties but capable of activating T-cells and inducing T s cells.
Claim 3
Dependent Claim
28 words
The method of claim 1 wherein said grass pollen antigen is antigen B, antigen D, antigen D 1 , antigen D 2 , or antigen D 3 .
Claim 4
Dependent Claim
31 words
The method of claim 1 wherein said oxidative conditions also result in the rearrangement and ring scission of the hydroxy aromatic ring present in the tyrosine moiety of said poly-peptide T.
Claim 5
Dependent Claim
34 words
The method of claim 1 wherein the extent of said oxidation is sufficient to result in a modified antigen incapable of reacting with antibodies directed against the antigenic determinant structure of said starting antigen.
Claim 6
Dependent Claim
24 words
The method of claim 4 wherein said oxidative conditions are such that substantial modification of the amino acid content of said antigen is avoided.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein said grass pollen is photooxidized.
Claim 8
Dependent Claim
27 words
The method of claim 6 wherein said photo-oxidiation is effected by saturating an aqueous solution of said antigen with oxygen and then exposing said solution to light.
Claim 9
Dependent Claim
113 words
An immunotherapeutic composition suitable for inhibiting allergic reactions in humans and lower animals comprising a modified grass pollen antigen and an inert carrier therefor in unit dosage form, said modified antigen being the product of a method comprising subjecting a quercitin moiety containing grass pollen antigen or fragment thereof having the structure: ##STR5## wherein: G represents glucose, and T represents threonine or a poly-peptide linked to said structure through a threonine molecule; to oxidative conditions such that the said dihydroxy aromatic rings undergo rearrangement and ring scission without substantially modifying said threonine or polypeptide to yield a product substantially devoid of antigenic properties but capable of activating T-cells and inducing T s cells.
Claim 10
Dependent Claim
113 words
An immunotherapeutic composition suitable for inhibiting allergic reactions in humans and lower animals comprising a modified grass pollen antigen and an inert carrier therefor in unit dosage form, said modified antigen being the product of a method comprising subjecting a quercitin moiety containing grass pollen antigen or fragment thereof having the structure: ##STR5## wherein: G represents glucose, and T represents threonine or a poly-peptide linked to said structure through a threonine molecule; to oxidative conditions such that the said dihydroxy aromatic rings undergo rearrangement and ring scission without substantially modifying said threonine or polypeptide to yield a product substantially devoid of antigenic properties but capable of activating T-cells and inducing T s cells.
Claim 11
Dependent Claim
28 words
The composition of claim 8 wherein said grass pollen antigen is antigen B, antigen D, antigen D 1 , antigen D 2 , or antigen D 3 .
Claim 12
Dependent Claim
31 words
The composition of claim 8 wherein said oxidative conditions also result in the rearrangement and ring scission of the hydroxy aromatic ring present in the tyrosine moiety of said poly-peptide T.
Claim 13
Dependent Claim
34 words
The composition of claim 8 wherein the extent of said oxidation is sufficient to result in a modified antigen incapable of reacting with antibodies directed against the antigenic determinant structure of said starting antigen.
Claim 14
Dependent Claim
24 words
The composition of claim 11 wherein said oxidative conditions are such that substantial modification of the amino acid content of said antigen is avoided.
Claim 15
Dependent Claim
11 words
The composition of claim 8 wherein said grass pollen is photooxidized.
Claim 16
Dependent Claim
27 words
The composition of claim 13 wherein said photoodixation is effected by saturating an aqueous solution of said antigen with oxygen and then exposing said solution to light.
References Cited (Backward Citations)
US4140679A
Cited
US4158705A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-16 | 1984-09-15 | 1985-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-16 | 1988-09-15 | 1989-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-16 | 1992-09-15 | 1993-03-17 | $7700.00 | Window Closed / Lapsed |