Patent US4256725A successfully refreshed from Google Patents.
US4256725A
Expired / Lapsed
US
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Preparation of solid substrate containing receptor and labeled form of ligand for assays
Assignee: Becton Dickinson and Co
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Filing: 1978-02-21
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Granted: 1981-03-17
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Expires: 1998-02-21
Abstract
Abstract A solid substrate is simultaneously contacted with a labeled form of a ligand to be assayed, a receptor for the ligand to be assayed and a solution of an ionic salt to produce a solid substrate which contains the labeled form of the ligand and the receptor. In a subsequent assay for the ligand, the solid substrate is contacted with a sample containing the ligand, whereby the labeled form of the ligand is available for equilibration with the receptor in competition with the ligand to be assayed.
Inventors
- 👤 Herman Rutner
- 👤 Thomas F. Dodd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 07:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N2470/00; G01N2470/10; G01N2470/12; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/817; Y10S436/826
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
113 words
A process for preparing an article suitable for use in a solid phase assay, comprising: contacting a solid substrate with a mixture of the labeled form of a ligand to be assayed, a receptor for the ligand and a solution of an ionic salt having an ionic strength of at least 0.5, and no greater than 10.0 whereby the receptor and labeled form of the ligand are placed on the solid substrate, said labeled form of the ligand being reversibly placed on the solid substrate, whereby during an assay for the ligand, the labeled form of the ligand is available for equilibration with the receptor in competition with the ligand to be assayed.
Claim 2
Dependent Claim
113 words
A process for preparing an article suitable for use in a solid phase assay, comprising: contacting a solid substrate with a mixture of the labeled form of a ligand to be assayed, a receptor for the ligand and a solution of an ionic salt having an ionic strength of at least 0.5, and no greater than 10.0 whereby the receptor and labeled form of the ligand are placed on the solid substrate, said labeled form of the ligand being reversibly placed on the solid substrate, whereby during an assay for the ligand, the labeled form of the ligand is available for equilibration with the receptor in competition with the ligand to be assayed.
Claim 3
Dependent Claim
11 words
The process of claim 1 wherein the labeled ligand is radiolabeled.
Claim 4
Dependent Claim
14 words
The process of claim 2 wherein the solid substrate is a plastic test tube.
Claim 5
Dependent Claim
33 words
The process of claim 1 wherein the solution of an ionic salt contains at least one member of the group consisting of water soluble salts of ammonium and alkali and alkaline earth metals.
Claim 6
Dependent Claim
23 words
The process of claim 4 wherein a mixture of the receptor and labeled form of the ligand are incubated prior to the contacting.
Claim 7
Dependent Claim
20 words
The process of claim 3 wherein the contacting is effected at a temperature of from 0° C. to 37° C.
Claim 8
Dependent Claim
23 words
The process of claim 6 wherein the ligand which is in labeled form is selected from the group consisting of antigens and haptens.
Claim 9
Dependent Claim
23 words
The process of claim 7 wherein a mixture of the receptor and labeled form of the ligand are incubated prior to the contacting.
Claim 10
Dependent Claim
33 words
The process of claim 7 wherein the solution of an ionic salt contains at least one member of the group consisting of water soluble salts of ammonium and alkali and alkaline earth metals.
Claim 11
Dependent Claim
17 words
An article suitable for use in a solid phase assay produced by the process of claim 1.
Claim 12
Dependent Claim
53 words
In a solid phase assay for a ligand in a sample wherein the sample is contacted with a solid substrate including a labeled form of the ligand and a receptor for the ligand, the improvement comprising: employing in the assay as said solid substrate an article produced by the process of claim 1.
Claim 13
Dependent Claim
24 words
The process of claim 5 wherein the preincubated mixture and the ionic solution are simultaneously applied to the solid substrate to provide said mixture.
Claim 14
Dependent Claim
24 words
The process of claim 5 wherein the preincubated mixture and the ionic solution are sequentially applied to the solid substrate to provide said mixture.
Claim 15
Dependent Claim
32 words
The process of claim 1 wherein the labeled form of the ligand, the receptor and the solution of an ionic salt are sequentially applied to the solid substrate to provide the mixture.
Claim 16
Dependent Claim
31 words
The process of claim 1 wherein the labeled form of the ligand, the receptor, and the solution of an ionic salt are simultaneously applied to the substrate to provide said mixture.
Claim 17
Dependent Claim
25 words
The process of claim 1 wherein the solution of an ionic salt has an ionic strength of at least 1.0 and no greater than 10.
Claim 18
Dependent Claim
13 words
The process of claim 4 wherein the solid substrate is a polymeric substrate.
Claim 19
Dependent Claim
18 words
The process of claim 17 wherein the polymer is selected from the group consisting of polystyrene and polypropylene.
Claim 20
Dependent Claim
32 words
The process of claim 18 wherein the receptor is a receptor for a conjugated bile acid and the labeled form of the ligand is a labeled form of a conjugated bile acid.
Claim 21
Dependent Claim
18 words
The process of claim 19 wherein the solution of an ionic salt contains ammonium sulfate and sodium chloride.
References Cited (Backward Citations)
US4017597A
Cited
US3951748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-16 | 1984-09-15 | 1985-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-16 | 1988-09-15 | 1989-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-16 | 1992-09-15 | 1993-03-17 | $7700.00 | Window Closed / Lapsed |