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US4256724A
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US
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Method for non-covalent coating of antibodies on solid substrates
Assignee: Becton Dickinson and Co
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Filing: 1978-02-21
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Granted: 1981-03-17
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Expires: 1998-02-21
Abstract
Abstract Antibodies to lipophilic haptens and antigens, such as the antibodies of bile acids are non-covalently coated on a solid substrate for use in solid phase immunoassays by including in the antibody coating solution an inorganic salt, such as ammonium sulfate, to increase the ionic strength of the solution.
Inventors
- 👤 Herman Rutner
- 👤 Thomas F. Dodd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/823
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
83 words
A process for coating an antibody on a solid substrate, comprising: contacting a solid substrate with antibody solution, said antibody being at least one member selected from the group consisting of antibodies to lipophilic haptens and antibodies to lipophilic antigens, said solution having at least one salt dissolved therein to provide a solution having an ionic strength of at least 0.5 and no greater than 10.0; and separating said solution and said solid substrate to provide said solid substrate coated with said antibody.
Claim 2
Dependent Claim
83 words
A process for coating an antibody on a solid substrate, comprising: contacting a solid substrate with antibody solution, said antibody being at least one member selected from the group consisting of antibodies to lipophilic haptens and antibodies to lipophilic antigens, said solution having at least one salt dissolved therein to provide a solution having an ionic strength of at least 0.5 and no greater than 10.0; and separating said solution and said solid substrate to provide said solid substrate coated with said antibody.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the ionic strength of the solution is at least 1.0.
Claim 4
Dependent Claim
30 words
The process of claim 1 wherein the salt is at least one member selected from the group consisting of water soluble salts of ammonium, alkali metals and alkaline earth metals.
Claim 5
Dependent Claim
15 words
The process of claim 1 wherein said antibody is an antibody to a bile acid.
Claim 6
Dependent Claim
14 words
The process of claim 4 wherein the solid substrate is a plastic test tube.
Claim 7
Dependent Claim
21 words
The process of claim 4 wherein the antibody solution has an antibody titer of from 1:10 3 to 1:10 6 .
Claim 8
Dependent Claim
18 words
The process of claim 6 wherein the ionic strength is at least 1.0 and no greater than 10.
Claim 9
Dependent Claim
28 words
An article suitable for solid phase assay of bile acid, comprising: a solid substrate and antibody to bile acid, said antibody being non-covalently coated on the solid substrate.
Claim 10
Dependent Claim
28 words
An article suitable for solid phase assay of bile acid, comprising: a solid substrate and antibody to bile acid, said antibody being non-covalently coated on the solid substrate.
Claim 11
Dependent Claim
13 words
The article of claim 8 wherein the bile acid is conjugated bile acid.
Claim 12
Dependent Claim
18 words
A solid substrate non-covalently coated with an antibody to bile acid produced by the process of claim 1.
Claim 13
Dependent Claim
14 words
The solid substrate of claim 10 wherein the antibody is to conjugated bile acid.
Claim 14
Dependent Claim
25 words
In an assay for a bile acid, the improvement comprising: effecting said assay in the solid phase with an article as defined in claim 8.
Claim 15
Dependent Claim
11 words
The assay of claim 12 wherein the assay is a radioimmunoassay.
Claim 16
Dependent Claim
13 words
The assay of claim 13 wherein the assay is for conjugated bile acid.
Claim 17
Dependent Claim
16 words
The assay of claim 14 wherein the assay is effected with cholylglycine histamine 125 I tracer.
Claim 18
Dependent Claim
24 words
In an assay for bile acid, the improvement comprising: effecting said assay in the solid phase with an article as defined in claim 10.
Claim 19
Dependent Claim
11 words
The assay of claim 16 wherein the assay is a radioimmunoassay.
Claim 20
Dependent Claim
13 words
The assay of claim 17 wherein the assay is for conjugated bile acid.
Claim 21
Dependent Claim
16 words
The assay of claim 18 wherein the assay is effected with cholylglycine histamine 125 I tracer.
Claim 22
Dependent Claim
30 words
The process of claim 4 wherein the salt is at least one member selected from the group consisting of water soluble salts of ammonium, alkali metals and alkaline earth metals.
Claim 23
Dependent Claim
15 words
The process of claim 20 wherein the antibody is an antibody to conjugated bile acid.
Claim 24
Dependent Claim
17 words
An article suitable for a solid phase assay produced in accordance with the process of claim 1.
Claim 25
Dependent Claim
52 words
The process of claim 1 wherein the antibody is an antibody to bile acid, the solution is buffered to a pH of from 5.0 to 10.0, the antibody titer of the solution is from 1:10 3 to 1:10 6 , and the ionic strength of the solution is from 0.5 to 10.0.
Claim 26
Dependent Claim
11 words
The process of claim 23 wherein the substrate is a polymer.
Claim 27
Dependent Claim
18 words
The process of claim 24 wherein the polymer is selected from the group consisting of polystyrene and polypropylene.
Claim 28
Dependent Claim
18 words
An article suitable for the solid phase assay of bile acid produced by the process of claim 25.
Claim 29
Dependent Claim
32 words
The process of claim 23 wherein the inorganic salt is at least one member selected from the group consisting of ammonium, potassium, and sodium sulfate alone or in admixture with sodium chloride.
Claim 30
Dependent Claim
15 words
The process of claim 3 wherein the antibody is an antibody to T 4 .
Claim 31
Dependent Claim
13 words
The process of claim 3 wherein the antibody is an antibody to insulin.
Claim 32
Dependent Claim
14 words
The process of claim 1 wherein the ionic strength is no greater than 5.
Claim 33
Dependent Claim
14 words
The process of claim 3 wherein the ionic strength is no greater than 5.
References Cited (Backward Citations)
US4017597A
Cited
US3951748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-16 | 1984-09-15 | 1985-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-16 | 1988-09-15 | 1989-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-16 | 1992-09-15 | 1993-03-17 | $7700.00 | Window Closed / Lapsed |