Patent US4253995A successfully refreshed from Google Patents.
US4253995A
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Immunochemical conjugates: method and composition
Assignee: Scripps Research Institute
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Filing: 1980-02-11
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Granted: 1981-03-03
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Expires: 2000-02-11
Abstract
Abstract Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.
Inventors
- 👤 David H. Katz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:50
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3823; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; Y; Y10; Y10S; Y10S424/00; Y10S424/805; Y10S424/81; Y10S436/00; Y10S436/823; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and protein comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated protein by affinity chromatography on an avidin-Sepharose chromatographic column.
Claim 2
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and protein comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated protein by affinity chromatography on an avidin-Sepharose chromatographic column.
Claim 3
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and antigen comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated antigen by affinity chromatography on an avidin-Sepharose chromatographic column.
Claim 4
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and antigen comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated antigen by affinity chromatography on an avidin-Sepharose chromatographic column.
Claim 5
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and insulin comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated insulin by affinity chromatography on an avidin-Sepharose chromatographic column.
Claim 6
Independent Claim
41 words
The method of purifying conjugate of D-glutamic acid-D-lysine copolymer and insulin comprising reacting said copolymer with biotin before forming said conjugate and, after formation of said conjugate, separating said conjugate from non-conjugated insulin by affinity chromatography on an avidin-Sepharose chromatographic column.
References Cited (Backward Citations)
US3794630A
Cited
US4191668A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-02 | 1984-09-01 | 1985-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-02 | 1988-09-01 | 1989-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-02 | 1992-09-01 | 1993-03-03 | $7700.00 | Window Closed / Lapsed |