Patent US4247646A successfully refreshed from Google Patents.
US4247646A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Laboratory apparatus for cloning mammalian cells
Assignee: United States Department of Health and Human Services
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Filing: 1978-09-12
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Granted: 1981-01-27
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Expires: 1998-09-12
Abstract
Abstract Laboratory apparatus for cloning mammalian cells includes a support plate and a plurality of tubular cylinders fixedly secured within the plate and projecting from one face thereof. All of the cylinders, with the exception of one pair, are disposed within a concentrated array defined within a predetermined sector region of the plate, the remaining pair of cylinders being located remote from the sector array yet equidistantly spaced from each other and from the sector array so as to complete the distribution pattern. A substantially C-shaped ring member is fixedly secured to, or integrally formed with, the opposite face of the support plate, in order to facilitate maneuverability of the assemblage when placing the same onto a Petri dish for isolating the colonies developing thereon. The plate and ring member are fabricated from transparent polycarbonate in order to permit the colonies to be identified or viewed by background lighting techniques. The distal ends of the isolating cylinders are also sharply beveled in order to cut into or penetrate the Petri dish so as to sealingly isolate the colonies.
Inventors
- 👤 John J. Berky
- 👤 John Hunziker, Jr.
- 👤 Laurence A. Zolotor
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:56
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/22; C12M23/02; C12M23/10; C12M33/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
93 words
Laboratory apparatus for cloning mammalian cells developing upon the surface of a Petri dish, comprising: a support plate; and a plurality of tubular cylinders fixedly secured within said support plate and projecting from one surface thereof so as to be capable of surounding and isolating particular mammalian cells developing upon a Petri dish, said plurality of tubular cylinders being concentrated within a sector of said support plate so as to define a predetermined distribution pattern with two remote tubular cylinders disposed away from said sector for insuring said isolation of particular mammalian cells.
Claim 2
Dependent Claim
93 words
Laboratory apparatus for cloning mammalian cells developing upon the surface of a Petri dish, comprising: a support plate; and a plurality of tubular cylinders fixedly secured within said support plate and projecting from one surface thereof so as to be capable of surounding and isolating particular mammalian cells developing upon a Petri dish, said plurality of tubular cylinders being concentrated within a sector of said support plate so as to define a predetermined distribution pattern with two remote tubular cylinders disposed away from said sector for insuring said isolation of particular mammalian cells.
Claim 3
Dependent Claim
35 words
Laboratory apparatus as set forth in claim 1, wherein: said support plate is circular as seen in plan view; and said sector of said support plate subtends as 80° arcuate extent of said circular plate.
Claim 4
Dependent Claim
32 words
Laboratory apparatus as set forth in claim 2, wherein: said tubular cylinders are arranged in sets, the centers of said cylinders of each set being disposed along radial lines of said plate.
Claim 5
Dependent Claim
39 words
Laboratory apparatus as set forth in claim 1, wherein: the distal ends of said cylinders are sharply beveled such that the same are able to pierce said surface of said Petri dish so as to sealingly isolate said cells.
Claim 6
Dependent Claim
43 words
Laboratory apparatus as set forth in claim 1, further comprising: a pair of auxiliary tubular cylinders, located remote from said plurality of cylinders located within said plate sector and spaced equidistantly from each other and said sector, for supplementing said predetermined distribution pattern.
Claim 7
Dependent Claim
22 words
Laboratory apparatus as set forth in claim 1, further comprising: means for facilitating the holding or grasping of said support plate-cylinders assemblage.
Claim 8
Dependent Claim
34 words
Laboratory apparatus as set forth in claim 6, wherein: said means comprises a substantially C-shaped ring member fixedly secured to the surface of said support plate opposite the surface from which said cylinders project.
Claim 9
Dependent Claim
26 words
Laboratory apparatus as set forth in claim 7, wherein: said C-shaped ring member is oriented so as to be symmetrically located with respect to said cylinders.
Claim 10
Dependent Claim
20 words
Laboratory apparatus as set forth in claim 7, wherein: said C-shaped ring member is fabricated from a transparent thermoplastic resin.
Claim 11
Dependent Claim
13 words
Laboratory apparatus as set forth in claim 9, wherein: said resin is polycarbonate.
Claim 12
Dependent Claim
19 words
Laboratory apparatus as set forth in claim 1, wherein: said support plate is fabricated from a transparent thermoplastic resin.
Claim 13
Dependent Claim
13 words
Laboratory apparatus as set forth in claim 11, wherein: said resin is polycarbonate.
Claim 14
Dependent Claim
16 words
Laboratory apparatus as set forth in claim 1, wherein: said cylinders are fabricated from stainless steel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-27 | 1984-07-28 | 1985-01-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-27 | 1988-07-28 | 1989-01-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-27 | 1992-07-28 | 1993-01-27 | $7700.00 | Window Closed / Lapsed |