Patent US4241186A successfully refreshed from Google Patents.
US4241186A
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Pectin culture media and method
Assignee: Conviron Inc
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Filing: 1978-12-18
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Granted: 1980-12-23
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Expires: 1998-12-18
Abstract
Abstract A method for preparing a gelled biological growth medium in a culture growth container which comprises combining a liquid growth medium including low methoxyl pectin and a multivalent metal cation material in the culture growth container. Preferably, the cation material is located on a support material, such as a filter pad or paper, and the liquid growth medium including the low methoxyl pectin material is poured over the support material while in the culture growth container.
Inventors
- 👤 Jonathan N. Roth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 15:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/045; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
123 words
A method for preparing a gelled biological growth medium having a pH from about 4 to about 9 in a culture growth container which comprises the steps of: a. providing a culture growth container, b. preparing a liquid growth medium including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content to gel the growth medium as the sole gelling agent; c. introducing a predetermined amount of calcium ions into an absorbent support material and placing the absorbent support material into the culture growth container; and d. combining the growth medium and the calcium ions in said culture growth container to produce gelling of the growth medium.
Claim 2
Independent Claim
123 words
A method for preparing a gelled biological growth medium having a pH from about 4 to about 9 in a culture growth container which comprises the steps of: a. providing a culture growth container, b. preparing a liquid growth medium including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content to gel the growth medium as the sole gelling agent; c. introducing a predetermined amount of calcium ions into an absorbent support material and placing the absorbent support material into the culture growth container; and d. combining the growth medium and the calcium ions in said culture growth container to produce gelling of the growth medium.
Claim 3
Dependent Claim
19 words
The method of claim 1 in which the low methoxyl pectin material has approximately a five percent methoxyl content.
Claim 4
Independent Claim
118 words
A method for preparing a gelled biological growth medium in a culture growth container which comprises the steps of: a. providing a culture growth container, said container including therein an absorbent support material containing calcium ions; b. providing a liquid growth medium having a pH from about 4 to about 9 including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content as the sole gelling agent for gelling the growth medium; and c. adding the growth medium to the culture growth container in an amount to provide gelling of the growth medium upon contact of the calcium ions with the pectin.
Claim 5
Independent Claim
118 words
A method for preparing a gelled biological growth medium in a culture growth container which comprises the steps of: a. providing a culture growth container, said container including therein an absorbent support material containing calcium ions; b. providing a liquid growth medium having a pH from about 4 to about 9 including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content as the sole gelling agent for gelling the growth medium; and c. adding the growth medium to the culture growth container in an amount to provide gelling of the growth medium upon contact of the calcium ions with the pectin.
Claim 6
Dependent Claim
43 words
The method of claim 3 in which the liquid growth medium includes a buffer selected from the group consisting of Na 2 HPO 4 , NaH 2 PO 4 , Na 3 PO 4 , NaHCO 3 and Na 2 CO 3 .
Claim 7
Dependent Claim
36 words
The method of claim 3 in which the calcium cations and liquid growth medium including pectin are combined in an amount of between about 15 and about 30 milligrams of calcium cations per gram of pectin.
Claim 8
Dependent Claim
19 words
The method of claim 3 in which the low methoxyl pectin material has approximately a five percent methoxyl content.
Claim 9
Dependent Claim
36 words
The method of claim 6 in which the calcium cations and liquid growth medium including pectin are combined in an amount of between about 15 and about 30 milligrams of calcium cations per gram of pectin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-23 | 1984-06-23 | 1984-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-23 | 1988-06-23 | 1988-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-23 | 1992-06-23 | 1992-12-23 | $7700.00 | Window Closed / Lapsed |