US4241177A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Propanolol antigen conjugates and antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1978-08-28
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Granted: 1980-12-23
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Expires: 1998-08-28
Abstract
Abstract Compounds are provided which can be used in the preparation of reagents useful for quantitatively determining the presence of beta -adrenergic blocking agents. These compounds contain a derivative of the blocking agent which is linked to an antigen or enzyme, the antigen conjugates being useful for preparing antibodies, and the enzyme conjugates being useful as detectors in an immunoassay as well. Additionally carboxylic acids, esters and aldehydes which are derivatives of the blocking agents are provided which are useful in preparing the above conjugates.
Inventors
- 👤 Prithipal Singh
- 👤 Marcel R. Pirio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 20:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; C; C12; C12N; C12N9/00; C12N9/96; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S436/00; Y10S436/815; Y10S436/822; Y10S436/826
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
59 words
A compound of the formula: ##STR4## wherein: R is an aliphatic linking group of from 1 to 4 carbon atoms; X and Z are taken together to form oxo or can each be hydrogen; A is an antigenic poly(amino acid) or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000.
Claim 2
Dependent Claim
59 words
A compound of the formula: ##STR4## wherein: R is an aliphatic linking group of from 1 to 4 carbon atoms; X and Z are taken together to form oxo or can each be hydrogen; A is an antigenic poly(amino acid) or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000.
Claim 3
Dependent Claim
13 words
A compound according to claim 1 wherein A is an antigenic poly(amino acid).
Claim 4
Dependent Claim
11 words
A compound according to claim 1 wherein A is an enzyme.
Claim 5
Dependent Claim
12 words
A compound according to claim 3 wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 6
Dependent Claim
34 words
A compound of the following formula: ##STR5## wherein: A 1 is an antigenic poly(amino acid); and n 1 is on the average from 1 to the molecular weight of A 1 divided by 1000.
Claim 7
Dependent Claim
34 words
A compound of the following formula: ##STR5## wherein: A 1 is an antigenic poly(amino acid); and n 1 is on the average from 1 to the molecular weight of A 1 divided by 1000.
Claim 8
Dependent Claim
19 words
A compound according to claim 5 wherein said antigenic poly(amino acid) is bovine serum albumin or bovine gamma globulin.
Claim 9
Dependent Claim
32 words
A compound of the following formula: ##STR6## wherein: A 1 is an enzyme; and n 1 is on the average from 1 to the molecular weight of A 1 divided by 1000.
Claim 10
Dependent Claim
32 words
A compound of the following formula: ##STR6## wherein: A 1 is an enzyme; and n 1 is on the average from 1 to the molecular weight of A 1 divided by 1000.
Claim 11
Dependent Claim
12 words
A compound according to claim 7 wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 12
Dependent Claim
31 words
A compound of the formula ##STR7## wherein A 1 is an antigenic poly(amino acid) or enzyme; and n 1 is one to the molecular weight of A 1 divided by 1,000.
Claim 13
Dependent Claim
31 words
A compound of the formula ##STR7## wherein A 1 is an antigenic poly(amino acid) or enzyme; and n 1 is one to the molecular weight of A 1 divided by 1,000.
Claim 14
Dependent Claim
32 words
An antibody prepared by administering to a host animal an antigen conjugate of a propranolol derivative having the formula of claim 1, and which has sites capable of specifically binding to propranolol.
Claim 15
Dependent Claim
109 words
A method for determining the presence of a drug which is propranolol in a medium suspected of containing said drug which comprises: forming, at a pH in the range of about 5-10 and at a temperature of from about 10°-50° C., an aqueous solution of said medium, an enzyme conjugate of said drug according to claim 1, and antibody having sites capable of binding to said drug and said enzyme conjugate, said antibody being at a sufficient concentration to substantially reduce the enzymatic activity of said enzyme conjugate in the absence of said drug; and analyzing said solution for the enzymatic activity of said enzyme conjugate in said solution.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-23 | 1984-06-23 | 1984-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-23 | 1988-06-23 | 1988-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-23 | 1992-06-23 | 1992-12-23 | $7700.00 | Window Closed / Lapsed |