US4070492A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Propranolol assay
Assignee: Hoffmann La Roche Inc
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Filing: 1977-03-02
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Granted: 1978-01-24
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Expires: 1997-03-02
Abstract
Abstract New hapten compositions useful in preparing antigens which may be employed in eliciting antibodies useful in immunoassay, particularly radioimmunoassay for propranolol. Utilizing an enantiomer in formation of the hapten allows preparation of an antibody which is able to discriminate between enantiomers selectively.
Inventors
- 👤 Sidney Spector
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; C; C07; C07K; C07K16/00; C07K16/44; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/823; Y10S530/00; Y10S530/806; Y10S530/807
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
54 words
An antibody specific to propranolol and the enantiomers thereof, said antibody being prepared by innoculating a host animal with an antigen consisting essentially of a propranolol hapten of the formula ##STR14## wherein n is an integer from 2 to 6; said hapten being covalently bonded to an immunogenic carrier material through the carboxyl group.
Claim 2
Dependent Claim
54 words
An antibody specific to propranolol and the enantiomers thereof, said antibody being prepared by innoculating a host animal with an antigen consisting essentially of a propranolol hapten of the formula ##STR14## wherein n is an integer from 2 to 6; said hapten being covalently bonded to an immunogenic carrier material through the carboxyl group.
Claim 3
Dependent Claim
12 words
The antibody of claim 1 wherein in said hapten n is 2.
Claim 4
Dependent Claim
14 words
The antibody of claim 2 wherein the propranolol of said hapten is the l-enantiomer.
Claim 5
Independent Claim
102 words
A method for the assay of propranolol or enantiomers thereof in a sample which method comprises mixing said sample with a known amount of a labelled propranolol derivative and an antibody which will selectively complex with said propranolol or its enantiomers thereof, and with said labelled propranolol derivative, measuring the degree of binding of said labeled propranolol derivative with said antibody, and determining the amount of propranolol or enantiomers thereof present in such sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of propranolol or enantiomers thereof with fixed amounts of said labeled propranolol derivative.
Claim 6
Independent Claim
102 words
A method for the assay of propranolol or enantiomers thereof in a sample which method comprises mixing said sample with a known amount of a labelled propranolol derivative and an antibody which will selectively complex with said propranolol or its enantiomers thereof, and with said labelled propranolol derivative, measuring the degree of binding of said labeled propranolol derivative with said antibody, and determining the amount of propranolol or enantiomers thereof present in such sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of propranolol or enantiomers thereof with fixed amounts of said labeled propranolol derivative.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein a radiolabeled propranolol derivative is used.
Claim 8
Dependent Claim
29 words
The method of claim 5 wherein said assay is selective for the detection of l-propranolol and utilizes an antibody which will selectively complex only with said l-enantiomer of propranolol.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-23 | 1981-07-25 | 1982-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-23 | 1985-07-25 | 1986-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-23 | 1989-07-25 | 1990-01-24 | $7700.00 | Window Closed / Lapsed |