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Patent US4235966A successfully refreshed from Google Patents.
US4235966A
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Process for the production of polysaccharide
Assignee: Talres Development (N A) NV
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Filing: 1979-06-06
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Granted: 1980-11-25
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Expires: 1999-06-06
Abstract
Abstract A process for the production of polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues, comprises cultivating in a nutrient medium therefor a strain of Pseudomonas, which is non-pathogenic to humans, and which has been obtained by treating a non-mucoid species of Pseudomonas, which is non-pathogenic to humans, with a β-lactam or aminoglycoside antibiotic whereby a mucoid strain tolerant to said antibiotic was selected, and isolating from the medium the polysaccharide produced.
Inventors
- 👤 Trevor R. Jarman
- 👤 John R. W. Govan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:08
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/38; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/877
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
79 words
A process for the production of polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues, which comprises cultivating in a nutrient medium therefor a strain of Pseudomonas, which is non-pathogenic to humans, and which has been obtained by treating a non-mucoid species of Pseudomonas, which is non-pathogenic to humans, with a β-lactam or aminoglycoside antibiotic whereby a mucoid strain tolerant to said antibiotic was selected, and isolating from the medium the polysaccharide produced.
Claim 2
Dependent Claim
79 words
A process for the production of polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues, which comprises cultivating in a nutrient medium therefor a strain of Pseudomonas, which is non-pathogenic to humans, and which has been obtained by treating a non-mucoid species of Pseudomonas, which is non-pathogenic to humans, with a β-lactam or aminoglycoside antibiotic whereby a mucoid strain tolerant to said antibiotic was selected, and isolating from the medium the polysaccharide produced.
Claim 3
Dependent Claim
28 words
A process according to claim 1, in which the penicillin antibiotic is a carboxyalkyl or carboxyaralkyl derivative of penicillin, an ester thereof or a 3-halophenyl derivative of penicillin.
Claim 4
Dependent Claim
13 words
A process according to claim 2, in which the penicillin antibiotic is carbenicillin.
Claim 5
Dependent Claim
23 words
A process according to claim 1 in which the strain of Pseudomonas has been derived from P. mendocina, P. tabaci or P. putida.
Claim 6
Dependent Claim
35 words
A process according to claim 1, in which the strain used has been obtained by subjecting the mucoid species of Pseudomonas to culture on a selective medium containing a generally inhibitory concentration of the antibiotic.
Claim 7
Dependent Claim
23 words
A process according to claim 5 in which carbenicillin had been included in the selective medium at a concentration of about 600 μg/ml.
Claim 8
Dependent Claim
28 words
A process according to claim 5 using P. mendocina or P. putida, selected by indicating carbenicillin the selective medium at a concentration of from 500 to 600 μg/ml.
References Cited (Backward Citations)
US3346463A
Cited
US3856625A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |