US3856625A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of polysaccharide
Assignee: Tate and Lyle PLC
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Filing: 1973-05-29
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Granted: 1974-12-24
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Expires: 1993-05-29
Abstract
Abstract An alginate-type polysaccharide is obtained by the aerobic cultivation of a bacterium of the species Azotobacter vinelandii in an aqueous nutrient medium containing sources of carbon, molybdenum, iron, mangesium, potassium, sodium, sulfate, calcium and phosphate. The carbon source comprises at least one monosaccharide or disaccharide. Contrary to normal culture conditions for this bacterium, for good polysaccharide production, the phosphate concentration in the nutrient medium must be 0.1-0.8 millimolar, preferably 0.2-0.6 millimolar; and the pH of the medium must be maintained within the range of from 7.0 to 8.2, preferably 7.3 to 7.9.
Inventors
- 👤 F Imrie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:13
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/006; C08B37/0084; C12; C12P; C12P19/00; C12P19/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/831
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
162 words
A PROCESS FOR THE PRODUCTION OF A POLYSACCHARIDE CONSISTING OF A PARTIALLY ACETYLATED VARIABLE BLOCK COPOLYMER OF 1-4 LINKED D-MANURONIC ACID AND L-GULURONIC ACID RESIDUES, WHICH PROCESS COMPRISES THE STEPS OF: A. INOCULATING AN AQUEOUS FINAL CULTURE MEDIUM WITH A BACTERIUM OF THE SPIECES AZOTOBACTER VINELANDII, SAID MEDIUM HAVING A PH IN THE RANGE OF FROM ABOUT 7..0 TO AOUT 8.2 AND CONTAINING AS ESSENTIAL INGREDIENTS AT LEAST ONE CARBON SOURCE SELECTED FROM THE GROUP CONSISTING OF MONOSACCHARIDES AND DISACCHARIDES, AT LEAST ONE PHOSPHATE SOURCE IN AN AMOUNT SUCH THAT THE CONCENTRATION OF PHOSPHATE IN THE MEDIUM IS IN THE RANGE OF FROM ABOUT 0.1 TO ABOUT 0.8 MILLIMOLAR, AND SOURCES OF MOLYBDENUM, IRON, MAGNESUIM, POTASSIUM, SODIUM, CALCIUM AND SULFATE; B. CULTIVATING SAID BACTERIUM IN SAID MEDIUM UNDER AEROBIC CONDITIONS, WHILE MAINTAINING THE PH OF THE MEDIUM WITHIN THE RANGE OF FROM ABOUT 7.0 TO ABOUT 8.2, UNTIL A SUBSTANTIAL FORMATION OF POLYSACCHARIDE HAS OCCURED; AND C. RECOVERING A POLYSACCHARIDE-CONTAINING CULTURE BROTH.
Claim 2
Dependent Claim
162 words
A PROCESS FOR THE PRODUCTION OF A POLYSACCHARIDE CONSISTING OF A PARTIALLY ACETYLATED VARIABLE BLOCK COPOLYMER OF 1-4 LINKED D-MANURONIC ACID AND L-GULURONIC ACID RESIDUES, WHICH PROCESS COMPRISES THE STEPS OF: A. INOCULATING AN AQUEOUS FINAL CULTURE MEDIUM WITH A BACTERIUM OF THE SPIECES AZOTOBACTER VINELANDII, SAID MEDIUM HAVING A PH IN THE RANGE OF FROM ABOUT 7..0 TO AOUT 8.2 AND CONTAINING AS ESSENTIAL INGREDIENTS AT LEAST ONE CARBON SOURCE SELECTED FROM THE GROUP CONSISTING OF MONOSACCHARIDES AND DISACCHARIDES, AT LEAST ONE PHOSPHATE SOURCE IN AN AMOUNT SUCH THAT THE CONCENTRATION OF PHOSPHATE IN THE MEDIUM IS IN THE RANGE OF FROM ABOUT 0.1 TO ABOUT 0.8 MILLIMOLAR, AND SOURCES OF MOLYBDENUM, IRON, MAGNESUIM, POTASSIUM, SODIUM, CALCIUM AND SULFATE; B. CULTIVATING SAID BACTERIUM IN SAID MEDIUM UNDER AEROBIC CONDITIONS, WHILE MAINTAINING THE PH OF THE MEDIUM WITHIN THE RANGE OF FROM ABOUT 7.0 TO ABOUT 8.2, UNTIL A SUBSTANTIAL FORMATION OF POLYSACCHARIDE HAS OCCURED; AND C. RECOVERING A POLYSACCHARIDE-CONTAINING CULTURE BROTH.
Claim 3
Dependent Claim
46 words
A process according to claim 1, wherein the aqueous final culture medium inoculated with said bacterium contains at least one phosphate source in an amount such that the concentration of phosphate in the medium is in the range of from about 0.2 to about 0.6 millimolar.
Claim 4
Dependent Claim
31 words
A process according to claim 1, wherein said bacterium is cultivated in said medium while maintaining the pH of the medium within the range of from about 7.3 to about 7.9.
Claim 5
Dependent Claim
29 words
A process according to claim 1, wherein said bacterium is a strain of Azotobacter vinelandii selected from the group consisting of strains NCIB 8660, NCIB 8789 and NCIB 9068.
Claim 6
Dependent Claim
46 words
A process according to claim 1, wherein the aqueous final culture medium inoculated with said bacterium contains at least one calcium source in an amount such that the concentration of calcium in the medium is in the range of from about 0.02 to about 0.6 millimolar.
Claim 7
Dependent Claim
28 words
A process according to claim 1, wherein said culture medium contains at least one carbon source selected from the group consisting of sucrose, glucose, invert sugar and molasses.
Claim 8
Dependent Claim
30 words
A process according to claim 1, wherein the cultivation of said bacterium in said medium is performed at a temperature in the range of from about 25*C to about 30*C.
Claim 9
Dependent Claim
106 words
In a process for the production of polysaccharide by the aerobic cultivation of a bacterium of the species Azotobacter vinelandii in an aqueous culture medium containing as essential ingredients at least one carbon source selected from the group consisting of monosaccharides and disaccharides, and sources of phosphate, molybdenum, iron, magnesium, potassium, sodium, calcium and sulfate, the improvement which comprises providing at least one phosphate source in an amount such that the concentration of phosphate in the medium is in the range of from about 0.1 to about 0.8 millimolar and maintaining the pH of the medium within the range of from about 7.0 to about 8.2.
Claim 10
Dependent Claim
106 words
In a process for the production of polysaccharide by the aerobic cultivation of a bacterium of the species Azotobacter vinelandii in an aqueous culture medium containing as essential ingredients at least one carbon source selected from the group consisting of monosaccharides and disaccharides, and sources of phosphate, molybdenum, iron, magnesium, potassium, sodium, calcium and sulfate, the improvement which comprises providing at least one phosphate source in an amount such that the concentration of phosphate in the medium is in the range of from about 0.1 to about 0.8 millimolar and maintaining the pH of the medium within the range of from about 7.0 to about 8.2.
Claim 11
Dependent Claim
27 words
An improved process according to claim 8, wherein the concentration of phosphate in the medium is in the range of from about 0.2 to about 0.6 millimolar.
Claim 12
Dependent Claim
26 words
An improved process according to claim 8, wherein the pH of the culture medium is maintained within the range of from about 7.3 to about 7.9.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-23 | 1978-06-24 | 1978-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-23 | 1982-06-24 | 1982-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-23 | 1986-06-24 | 1986-12-24 | $7700.00 | Window Closed / Lapsed |