Patent US4230664A successfully refreshed from Google Patents.
US4230664A
Expired / Lapsed
US
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Test pack kit for immunoassay
Assignee: Technion Research and Development Foundation Ltd
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Filing: 1979-01-23
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Granted: 1980-10-28
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Expires: 1999-01-23
Abstract
Abstract A test pack kit for assaying for a ligand in a liquid medium. The kit comprises specially designed assay tubes, each tube having a cylindrical side arm which can be interconnected with the side arm of another assay tube. In addition, the pack kit contains antiserum; a conjugate of a metal labelling substance with the ligand; assay calibration standards--in vials containing known concentrations of unlabelled ligand; a separation agent in vials containing material used for separating the bound antibody--metal labelling complex from the unbound metal labelling substance and instrument calibration standards--in vials containing several known concentrations of the metal--labelling substance. Errors caused by pipetting or transferring of reagents from one container to another are practically eliminated.
Inventors
- 👤 Michael Cais
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 07:06
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5082; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/536; G01N33/537; Y; Y10; Y10S; Y10S436/00; Y10S436/801; Y10S436/807; Y10S436/808; Y10S436/81
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
113 words
A test pack kit for use in assaying a liquid medium for a ligand which comprises in a packaged combination: (a) at least two assay tubes, each tube having a cylindrical side arm which can be interconnected with the side arm of another assay tube to form a leak-proof connecting channel; (b) an antiserum; (c) a conjugate of a metal-labelling substance with said ligand; (d) a separation agent in vials containing material used for separating the bound antibody-metal labelling complex from the unbound metal labelling substance; (e) assay calibration standards-in vials containing known concentrations of unlabelled ligand, and (f) instrument calibration standards-in vials containing several containing several known concentrations of the metal-labelling substance.
Claim 2
Dependent Claim
113 words
A test pack kit for use in assaying a liquid medium for a ligand which comprises in a packaged combination: (a) at least two assay tubes, each tube having a cylindrical side arm which can be interconnected with the side arm of another assay tube to form a leak-proof connecting channel; (b) an antiserum; (c) a conjugate of a metal-labelling substance with said ligand; (d) a separation agent in vials containing material used for separating the bound antibody-metal labelling complex from the unbound metal labelling substance; (e) assay calibration standards-in vials containing known concentrations of unlabelled ligand, and (f) instrument calibration standards-in vials containing several containing several known concentrations of the metal-labelling substance.
Claim 3
Dependent Claim
16 words
A test pack kit according to claim 1, wherein the test pack kit contains assay buffer.
Claim 4
Dependent Claim
22 words
A test pack kit according to claim 1, wherein the pair assay tubes to be interconnected are made of differently coloured material.
Claim 5
Dependent Claim
23 words
A test pack kit according to claim 1, wherein the antiserum is in the form of a freeze dried cake in appropriate concentration.
Claim 6
Dependent Claim
22 words
A test pack kit according to claim 1, wherein the antiserum is bound to the assay-tube walls by absorption or covalently bound.
Claim 7
Dependent Claim
26 words
A test pack kit as in claim 1, wherein said metal-labelled conjugate comprises said ligand or a specific binding analog thereof bound to a metal organo-derivative.
Claim 8
Dependent Claim
86 words
A test pack kit as in claim 1, wherein said metal organo-derivative is represented by the general formula M.sub.m L.sub.n.sup.1 L.sub.o.sup.2 L.sub.p.sup.3 L.sub.q.sup.4 L.sub.r.sup.5 L.sub.s.sup.6 wherein M is said metal atom, L 1 through L 6 are ligands or specific binding analogs thereof, which may be the same or different, m is an integer between 1 and 10, and n, o, p, q, r and s are integers between 0 and 12 provided that their sum does not exceed the coordination number of M m .
Claim 9
Dependent Claim
21 words
A test pack kit as in claim 1, wherein said metal organo-derivative is an organo-metallic compound or a metal coordination complex.
Claim 10
Dependent Claim
17 words
A test pack kit as in claim 8, wherein said organo-metallic compound is characterized by covalent bonding.
Claim 11
Dependent Claim
16 words
A test pack kit as in claim 8, wherein said organo-metallic compound is characterized by pi-bonding.
Claim 12
Dependent Claim
21 words
A test pack kit as in claim 1, wherein said specific binding partner of said ligand is in an insoluble form.
Claim 13
Dependent Claim
31 words
A test pack kit as in claim 1, which additionally comprises a container of an antibody to said specific binding partner of said ligand, which antibody is in an insoluble form.
Claim 14
Dependent Claim
44 words
A test pack kit as in claim 1, wherein the metal-labelling substance is a transition metal selected from the group consisting of I B, II B, III B, IV B, V B, VI B, VII B, and VIII of the Periodic Table of elements.
Claim 15
Dependent Claim
27 words
A test pack kit as in claim 13, wherein said metal is selected from the group consisting of Fe, Hg, Cr, Co, Pd, Au, Mn and Cu.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-28 | 1984-04-28 | 1984-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-28 | 1988-04-28 | 1988-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-28 | 1992-04-28 | 1992-10-28 | $7700.00 | Window Closed / Lapsed |