Compounds and methods

Claim 1

An antibody extracted from a mammal or bird after innoculation with a conjugate formed from a carrier molecule selected from the group natural protein, synthetic polypeptide, and other macromolecular immunogen of molecular weight greater than 2,000 and less than 500,000 and a compound selected from the group ##STR26## wherein M 1 is ##STR27## wherein R 5 is hydrogen or methyl; wherein L 1 is ##STR28## or a mixture of ##STR29## wherein R 3 and R 4 are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R 3 and R 4 is fluoro only when the other is hydrogen or fluoro; wherein Z 1 is (1) cis--CH═CH--CH 2 --(CH 2 )g--CH 2 --, (2) cis--CH═CH--CH 2 --(CH 2 )g--CF 2 --, (3) cis--CH 2 --CH═CH--(CH 2 )g--CH 2 --, (4) --(CH 2 ) 3 --(CH 2 )g--CH 2 --, (5) --(CH 2 ) 3 --(CH 2 )g--CF 2 --, (6) --CH 2 --O--CH 2 --(CH 2 )g--CH 2 --, (7) --C═C--CH 2 --(CH 2 )g--CH 2 --, (8) --CH 2 --C═C--(CH 2 )g--CH 2 --, ##STR30## wherein g is one, 2, or 3; wherein R 7 is ##STR31## wherein m is one to 5, inclusive; n is zero or one; T is chloro, fluoro, trifluoromethyl, alkyl of one to 3 carbon atoms, inclusive, or alkoxy of one to 3 carbon atoms, inclusive; and s is zero, one, 2 or 3, the various T's being the same or different, with the proviso that not more than two T's are other than alkyl, with the further proviso that R 7 is ##STR32## wherein T and s are as defined above, only when R 3 and R 4 are hydrogen or methyl, being the same or different; and wherein X 1 is --COOR 1 wherein R 1 is hydrogen or a pharmacologically acceptable cation.