Patent US2148566A successfully refreshed from Google Patents.
Patent US4212650A successfully refreshed from Google Patents.
US4212650A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic process
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1978-11-09
•
Granted: 1980-07-15
•
Expires: 1998-11-09
Abstract
Abstract A process for an in vitro determination of the immunization status of cells of human beings and animals by measuring in an electric field the changed mobility of charged indicator particles which have been treated with a medium of lymphocytes incubated with a synthetic peptide of high basicity.
Inventors
- 👤 Hartwig W. Bauer
- 👤 Wolfgang Ax
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:08
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; Y; Y10; Y10S; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
52 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, subjecting the incubation mixture to an electric field, and measuring the migration speed of the lymphocytes in the electric field.
Claim 2
Dependent Claim
52 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, subjecting the incubation mixture to an electric field, and measuring the migration speed of the lymphocytes in the electric field.
Claim 3
Dependent Claim
85 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, combining the incubation mixture with a dispersion of indicator particles, said particles exhibiting a substantially uniform behavior in an electric field and being interactive with said incubation mixture whereby their behavior in the electric field is modified in a detectable manner, and measuring the migration speed of the particles in the electric field.
Claim 4
Dependent Claim
85 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, combining the incubation mixture with a dispersion of indicator particles, said particles exhibiting a substantially uniform behavior in an electric field and being interactive with said incubation mixture whereby their behavior in the electric field is modified in a detectable manner, and measuring the migration speed of the particles in the electric field.
Claim 5
Dependent Claim
95 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, separating the lymphocytes from the incubation mixture, combining the lymphocyte-free supernatant of the incubation mixture with a dispersion of indicator particles, said particles exhibiting a substantially uniform behavior in an electric field and being interactive with said supernatant whereby their behavior in the electric field is modified in a detectable manner, and measuring the migration speed of the particles in the electric field.
Claim 6
Dependent Claim
95 words
An in vitro diagnostic method, which comprises incubating lymphocytes with a synthetic peptide of high basicity whereby the behavior of the lymphocytes in an electric field is modified in a detectable manner, separating the lymphocytes from the incubation mixture, combining the lymphocyte-free supernatant of the incubation mixture with a dispersion of indicator particles, said particles exhibiting a substantially uniform behavior in an electric field and being interactive with said supernatant whereby their behavior in the electric field is modified in a detectable manner, and measuring the migration speed of the particles in the electric field.
Claim 7
Dependent Claim
31 words
A method as claimed in claim 1, 2 or 3, wherein the synthetic peptide of high basicity is a synthetic polypeptide consisting essentially of lysine, arginine, ornithine, or a mixture thereof.
Claim 8
Dependent Claim
24 words
A method as defined in claim 4 wherein the synthetic polypeptide consists essentially of poly-L-lysine, poly-L-arginine, poly-L-ornithine, or a heterogeneous or homogeneous mixture thereof.
Claim 9
Dependent Claim
28 words
A method as defined in claim 5, wherein the synthetic peptide of high basicity is poly-L-lysine of a molecular weight in the range of from 750 to 100,000.
Claim 10
Dependent Claim
24 words
A method as defined in claim 6, wherein the synthetic peptide is of a molecular weight in the range of from 750 to 5,000.
Claim 11
Dependent Claim
19 words
A method as defined in claim 2 or 3, wherein the indicator particles are denatured human or animal erythrocytes.
Claim 12
Dependent Claim
18 words
A method as defined in claim 8, wherein the indicator particles are tanned erythrocytes stabilized with sulfosalicylic acid.
Claim 13
Dependent Claim
48 words
A method as defined in claim 3, wherein the supernatant is added to the dispersion of indicator particles in the proportion of from 1 to 10 ml of supernatant to from 0.1 to 1.0 ml of said dispersion containing from 1×10 7 to 5×10 7 particles per milliliter.
Claim 14
Dependent Claim
16 words
A method as defined in claim 2 or 3 wherein the indicator particles are liposome-membrane particles.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-15 | 1984-01-14 | 1984-07-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-15 | 1988-01-14 | 1988-07-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-15 | 1992-01-14 | 1992-07-15 | $7700.00 | Window Closed / Lapsed |