US4081241A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic process
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1975-08-28
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Granted: 1978-03-28
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Expires: 1995-08-28
Abstract
Abstract An in vitro method for the diagnosis of immunization states which involves measuring, in an electric field, the changed mobility of charged indicator particles, other than macrophages, which particles have been treated with (1) a suspension of lymphocytes that have been incubated with an antigen or (2) the cell-free supernatant of such a suspension of incubated lymphocytes.
Inventors
- 👤 Franz Porzsolt
- 👤 Christoph Tautz
- 👤 Rudolf Schmidtberger
- 👤 Wolfgang Ax
- 👤 Burkhard Enders
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 02:09
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
95 words
In an in vitro diagnostic method wherein lymphocytes are incubated with antigens, the incubation mixture or the cell-free supernatant thereof is added to a dispersion of macrophages, and the migration speed of the macrophages is measured in an electric field, the improvement wherein said incubation mixture or cell-free supernatant is added to a dispersion of particles other than macrophages, said particles being interactive with said incubation mixture or supernatant whereby their behavior in the electric field is modified in a detectable manner, and said particles further showing a substantially uniform behavior in the electric field.
Claim 2
Dependent Claim
95 words
In an in vitro diagnostic method wherein lymphocytes are incubated with antigens, the incubation mixture or the cell-free supernatant thereof is added to a dispersion of macrophages, and the migration speed of the macrophages is measured in an electric field, the improvement wherein said incubation mixture or cell-free supernatant is added to a dispersion of particles other than macrophages, said particles being interactive with said incubation mixture or supernatant whereby their behavior in the electric field is modified in a detectable manner, and said particles further showing a substantially uniform behavior in the electric field.
Claim 3
Dependent Claim
12 words
A method as in claim 1 wherein said particles are denatured erythrocytes.
Claim 4
Dependent Claim
17 words
A method as in claim 2 wherein said denatured erythrocytes are tanned erythrocytes stabilized with sulfosalicylic acid.
Claim 5
Dependent Claim
12 words
A method as in claim 1 wherein said particles are latex particles.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-27 | 1981-09-26 | 1982-03-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-27 | 1985-09-26 | 1986-03-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-27 | 1989-09-26 | 1990-03-28 | $7700.00 | Window Closed / Lapsed |