US4209588A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing new antitumor anthracycline antibiotics
Assignee: Microbial Chemistry Research Foundation
•
Filing: 1978-07-27
•
Granted: 1980-06-24
•
Expires: 1998-07-27
Abstract
Abstract New anthracycline glycosides designated MA 144-G1, -G2, -L,-S1, -N1, -U1 and -Y which inhibit the growth of gram-positive bacteria and mammalian tumors are produced by fermentation of certain species of Streptomyces and by the chemical or enzymatic conversion of certain anthracycline glycosides. New microbiological and chemical processes are also provided for preparation of the anthracycline glycosides MA 144-S2 and -U2 which have been found to be identical with the previously reported anthracyclines, marcellomycin and musettamycin.
Inventors
- 👤 Hamao Umezawa
- 👤 Tomio Takeuchi
- 👤 Toshikazu Oki
- 👤 Taiji Inui
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 02:33
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C07; C07H; C07H15/00; C07H15/20; C07H15/24; C07H15/252; C12P; C12P19/00; C12P19/44; C12P19/56; C12R; C12R2001/00; C12R2001/01; C12R2001/465; C12R2001/48; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/888
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
95 words
A process for producing MA 144-G1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-G1 is produced by said organism in said culture medium and recovering the MA 144-G1 from the culture medium substantially free of substances co-produced therewith.
Claim 2
Dependent Claim
95 words
A process for producing MA 144-G1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-G1 is produced by said organism in said culture medium and recovering the MA 144-G1 from the culture medium substantially free of substances co-produced therewith.
Claim 3
Dependent Claim
95 words
A process for producing MA 144-S1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-S1 is produced by said organism in said culture medium and recovering the MA 144-S1 from the culture medium substantially free of substances co-produced therewith.
Claim 4
Dependent Claim
95 words
A process for producing MA 144-S1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-S1 is produced by said organism in said culture medium and recovering the MA 144-S1 from the culture medium substantially free of substances co-produced therewith.
Claim 5
Dependent Claim
95 words
A process for producing MA 144-N1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3567, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-N1 is produced by said organism in said culture medium and recovering the MA 144-N1 from the culture medium substantially free of substances co-produced therewith.
Claim 6
Dependent Claim
95 words
A process for producing MA 144-N1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3567, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-N1 is produced by said organism in said culture medium and recovering the MA 144-N1 from the culture medium substantially free of substances co-produced therewith.
Claim 7
Dependent Claim
95 words
A process for producing MA 144-U1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-U1 is produced by said organism in said culture medium and recovering the MA 144-U1 from the culture medium substantially free of substances co-produced therewith.
Claim 8
Dependent Claim
95 words
A process for producing MA 144-U1 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-U1 is produced by said organism in said culture medium and recovering the MA 144-U1 from the culture medium substantially free of substances co-produced therewith.
Claim 9
Dependent Claim
95 words
A process for producing MA 144-Y which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-Y is produced by said organism in said culture medium and recovering the MA 144-Y from the culture medium substantially free of substances co-produced therewith.
Claim 10
Dependent Claim
95 words
A process for producing MA 144-Y which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-Y is produced by said organism in said culture medium and recovering the MA 144-Y from the culture medium substantially free of substances co-produced therewith.
Claim 11
Dependent Claim
95 words
A process for producing MA 144-G2 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-G2 is produced by said organism in said culture medium and recovering the MA 144-G2 from the culture medium substantially free of substances co-produced therewith.
Claim 12
Dependent Claim
95 words
A process for producing MA 144-G2 which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-G2 is produced by said organism in said culture medium and recovering the MA 144-G2 from the culture medium substantially free of substances co-produced therewith.
Claim 13
Dependent Claim
94 words
A process for producing MA 144-L which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-L is produced by said organism in said culture medium and recovering the MA 144-L from the culture medium substantially free of substances co-produced therewith.
Claim 14
Dependent Claim
94 words
A process for producing MA 144-L which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-L is produced by said organism in said culture medium and recovering the MA 144-L from the culture medium substantially free of substances co-produced therewith.
Claim 15
Dependent Claim
102 words
A process for producing the anthracycline glycoside MA 144-S2 of the formula ##STR15## which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-S2 is produced by said organism in said culture medium and recovering the MA 144-S2 from the culture medium substantially free of substances co-produced therewith.
Claim 16
Dependent Claim
102 words
A process for producing the anthracycline glycoside MA 144-S2 of the formula ##STR15## which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATCC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-S2 is produced by said organism in said culture medium and recovering the MA 144-S2 from the culture medium substantially free of substances co-produced therewith.
Claim 17
Dependent Claim
102 words
A process for producing the anthracycline glycoside MA 144-U2 of the formula ##STR16## which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATTC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-U2 is produced by said organism in said culture medium and recovering the MA 144-U2 from the culture medium substantially free of substances co-produced therewith.
Claim 18
Dependent Claim
102 words
A process for producing the anthracycline glycoside MA 144-U2 of the formula ##STR16## which comprises cultivating a Streptomyces strain selected from the group consisting of Streptomyces galilaeus MA 144-M1 (FERM P-2445, ATCC 31133), Streptomyces galilaeus ATTC 14969, Streptomyces sp. ME 505-HE1 (FERM P-3667, ATCC 31273), Streptomyces cinereoruber ATCC 19740, Streptomyces niveoruber ATCC 14971, Streptomyces antibioticus ATCC 8663 and Streptomyces purpurascens ATCC 25489 in an aqueous nutrient medium under submerged aerobic conditions until a substantial amount of MA 144-U2 is produced by said organism in said culture medium and recovering the MA 144-U2 from the culture medium substantially free of substances co-produced therewith.
References Cited (Backward Citations)
GB846130A
Cited
GB901830A
Cited
US3092550A
Cited
GB985598A
Cited
GB1188262A
Cited
US3616242A
Cited
US3590028A
Cited
US3803124A
Cited
US3686163A
Cited
GB1241750A
Cited
US3723411A
Cited
GB1426637A
Cited
US3992264A
Cited
US3864480A
Cited
US3988315A
Cited
US4039736A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-24 | 1983-12-24 | 1984-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-24 | 1987-12-24 | 1988-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-24 | 1991-12-24 | 1992-06-24 | $7700.00 | Window Closed / Lapsed |