Antibiotic danubomycin and process for its manufacture

Claim 2

PROCESS FOR THE MANUFACTURE OF THE NEW ANTIBIOTIC DANUBOMYCIN, WHEREIN STREPTOMYCES GRISEUS, STRAIN NRRL 2719, IN A NUTRIENT SOLUTION CONTAINING A SOURCE OF CARBON AND NITROGEN AND INORGANIC SALTS IS CULTRED UNDER AEROBIC CONDITIONS UNTIL THE NUTRIENT SOLUTION SHOWS AND ESSENTIAL ANTIBIOTIC ACITION, WHEREUPON THE NEW ANTIBIOTIC DANUBOMCIN IS ISOLATED.

Claim 3

A MEMBER SELECTED FROM THE GROUP CONSISTING OF THE ANTIBIOTIC DANUBOMYCIN, A REDDISH SUBSTANCE HAVING BASIC PROPERITIES, BEING SOLUBLE IN ALCOHOLS, KETONES, ESTERS, CHLORINATED HYDROCARBONS AND AROMATIC HYDROCARGONS AND PRACTICALLY INSOLUBLE IN PETROLETHER AND WATER, FORMING WITH ACIDS YELLOW SALTS OF GOOD-WATER-SOLUBILITY, EXHIBITING IN THE U.V.-SPECTRUM AND BAND AT 243 MU WITH SHOULDER AT 260 MU, AND SHOWING IN PAPER-CHOMATOGRAPHY THE FOLLOWING RF-VALUES: IN N-BUTANOL SATURATED WITH WATER 0.65 IN N-BUTANOL SATURATED WITH WATER WATER + 2% P-TOLUENE SULFONIC ACID + 2% PIPERIDINE 0.9. IN METHYL ISOBUTYL KETONE SATURATED WITH WATER 0.00 IN 80% ETHANOL WITH 1.5% SODIUM CHLORIDE, PAPER IMPREGNATED WITH 0.95-MOLAR SODIUM SULFATE SOLUTION AND 0.05-MOLAR SODIUM BISULFATE SOLUTION 0.5 IN BUTANOL/METHANOL/WATER, 4:1:2 0.67 IN WATER SATURATED WITH METHYL ISOBUTYL KETONE 0.05 AND 0.70 IN WATER SATURATED WITH METHYL ISOBBUTYL KETONE +1% P-TOLUENE SULFONIC ACID 0.08 IN 75% WATER+25% OF A MIXTURE OF 3 PARTS OF METHANOL AND 1 PAT OF ACETONE, BROUGHT TO PH 10.5 WITH AMMONIA AND NEUTRALIZED WITH PHOSPHORIC ACID TO PH 7.5 0.05 AND 0.70 AND ACID ADDITION SALTS THEREFO, SAID DANUBOMYCIN HAVING BEEN PRODUCED BY THE PROCESS OF CLAIM 1.