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Patent US4199450A successfully refreshed from Google Patents.
US4199450A
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Process of separating from an aqueous medium a protein or a morphologically organized unit by liquid exclusion chromatography
Assignee: Societe Nationale Elf Aquitaine Production SA
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Filing: 1978-04-26
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Granted: 1980-04-22
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Expires: 1998-04-26
Abstract
Abstract A novel process of separating from an aqueous medium at least one protein or at least one morphologically organized unit by liquid exclusion chromatography on a solid support previously passivated by treatment with an aqueous solution of a non-proteinic polymer. In this process, the chromatographic support is submitted, prior to the chromatographic operation, to a second passivation consisting in contacting the support with an aqueous solution of a protein which has a molecular weight lower than that of the protein to be separated, and which is adapted to be adsorbed by said support. The support and/or the liquid to be treated are sterilized with antiseptic agent selected from the group constituted by the lower aliphatic halogenated hydrocarbons.
Inventors
- 👤 Charles Dulout
- 👤 Andre Peyrouset
- 👤 Rene Panaris
- 👤 Claude Hannoun
- 👤 Jean Vincent
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/34; B01J; B01J20/00; B01J20/281; B01J20/282; B01J20/283; B01J20/30; B01J20/3085; C07K1/00; C07K1/14; C07K1/16; C07K1/20; C12; C12N; C12N9/00; C12N9/0004; C12N9/0065; A; A61; A61K; A61K39/00; B01J2220/00; B01J2220/50; B01J2220/54; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16022; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/826
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
74 words
A process of separating a protein from an aqueous medium by liquid exclusion chromatography on a solid porous support which comprises contacting said support with the following successive agents: (a) an aqueous solution of a non-proteinic polymer; (b) an aqueous solution of a protein whose molecular weight is substantially less than the molecular weight of the protein to be separated; (c) said aqueous medium containing the protein to be separated; and (d) an eluent.
Claim 2
Dependent Claim
74 words
A process of separating a protein from an aqueous medium by liquid exclusion chromatography on a solid porous support which comprises contacting said support with the following successive agents: (a) an aqueous solution of a non-proteinic polymer; (b) an aqueous solution of a protein whose molecular weight is substantially less than the molecular weight of the protein to be separated; (c) said aqueous medium containing the protein to be separated; and (d) an eluent.
Claim 3
Dependent Claim
47 words
The process of claim 1, wherein said solid support is selected from the group consisting of silica gel and an alkali metal silicate in the form of particles having dimensions comprised between 40 and 200 microns, with an average pore diameter comprised between 5 and 200 nm.
Claim 4
Dependent Claim
26 words
The process of claim 1, wherein said non-proteinic polymer is selected from the group consisting of polyethylene-glycol, polypropylene-glycol, polybutylene-glycol, polyvinyl alcohol, polyvinyl pyrrolidone and (polyethylene-polypropylene)-glycol copolymers.
Claim 5
Dependent Claim
37 words
The process of claim 3, wherein said non-proteinic polymer has a molecular weight of 5,000 to 30,000, and wherein the concentration of said polymer in said aqueous solution is comprised between 0.5 and 5 g per liter.
Claim 6
Dependent Claim
18 words
The process of claim 1, wherein said lower molecular weight protein has a molecular weight lower than 100,000.
Claim 7
Dependent Claim
38 words
The process of claim 5 wherein said lower molecular weight protein is selected from the group consisting of albumins, gelatins, peptones and products of degradation of polypeptides, the molecular weight of said protein being not higher than 50,000.
Claim 8
Dependent Claim
21 words
The process of claim 1, wherein said lower molecular weight protein solution contains 0.2 to 20% by weight of said protein.
Claim 9
Dependent Claim
75 words
The process of claim 1, wherein said chromatographic support is eluted by means of an aqueous buffer solution having a pH value comprised between 5.5 and 7.5, and wherein an antiseptic agent is added to said buffer solution, said antiseptic agent being constituted by at least one halogenated lower aliphatic hydrocarbon and added in a proportion comprised within the range of the solubility of said agent in water, said proportion being 0.1 to 10 g/l.
Claim 10
Dependent Claim
54 words
The process of claim 1, wherein an antiseptic agent constituted by at least one halogenated lower aliphatic hydrocarbon is added to the liquid submitted to chromatography, said antiseptic agent being added in a proportion comprised within the range of the solubility of the said agent in water, said proportion being 0.1 to 10 g/l.
Claim 11
Dependent Claim
43 words
The process of claim 8, wherein said antiseptic agent is selected from the group consisting of monohalogenomethane, dihalogenomethane, trihalogenomethane, tetrahalogenomethane, monohalogenoethane, dihalogenoethane, trihalogenoethane, tetrahalogenoethane, monohalogenopropane, dihalogenopropane, trihalogenopropane and tetrahalogenopropane, wherein the halogen is selected from the group consisting of chlorine, bromine and iodine.
Claim 12
Dependent Claim
43 words
The process of claim 9, wherein said antiseptic agent is selected from the group consisting of monohalogenomethane, dihalogenomethane, trihalogenomethane, tetrahalogenomethane, monohalogenoethane, dihalogenoethane, trihalogenoethane, tetrahalogenoethane, monohalogenopropane, dihalogenopropane; trihalogenopropane and tetrahalogenopropane, wherein the halogen is selected from the group consisting of chlorine, bromine and iodine.
Claim 13
Dependent Claim
20 words
The process of claim 8, wherein said antiseptic agent is chloroform used in a proportion of 1 to 7 g/l.
Claim 14
Dependent Claim
20 words
The process of claim 9, wherein said antiseptic agent is chloroform, used in a proportion of 1 to 7 g/l.
Claim 15
Dependent Claim
14 words
The process of claim 1, wherein the protein to be separated is a virus.
Claim 16
Dependent Claim
20 words
The process of claim 14, wherein the liquid submitted to exclusion chromatography is a virus solution of comparatively high concentration.
Claim 17
Dependent Claim
67 words
The process of claim 15, wherein said liquid submitted to chromatography is a concentrated virus solution obtained by adsorption of the virus on an unsoluble polyalkylene-glycol complex having a polymerization degree of 80-1,200, with a calcium salt, said adsorption being followed by the separation of the adsorbate--and its redissolution in an aqueous solution of ethylene--diaminotetracetic acid or in an aqueous solution of sodium salt of said acid.
Claim 18
Dependent Claim
46 words
The process of claim 1 wherein said support is contacted with an aqueous sterilized buffer solution having a pH of about 5.5 to 7.5 between steps (b) and (c) and wherein eluent is an aqueous sterilized buffer solution having a pH of about 5.5 to 7.5.
Claim 19
Independent Claim
187 words
A method of separating a virus from an aqueous medium containing said virus by liquid exclusion chromatography on a solid porous support wherein said support is silica gel or glass in the form of 40 to 200 microns particles having an average pore diameter of 5 to 200 nm, comprising subjecting said support to the following successive steps: (a) contact with an aqueous solution of a non-proteinic polymer selected from the group consisting of polyethylene glycol, polypropylene glycol, polybutylene glycol, polyvinyl alcohol, polyvinyl pyrrolidone and (polyethylene-polypropylene) glycol polymer, said polymer having a molecular weight of 5,000 to 30,000 and present at a concentration of 0.5 to 5 grams per liter; (b) contact with an aqueous solution containing 0.2 to 20% by weight of a protein whose molecular weight is lower than the molecular weight of the virus and does not exceed 100,000; (c) rinsing with an aqueous buffer sterilized solution having a pH of about 5.5 to 7.5; (d) contact with the aqueous medium containing the virus to be separated; and (e) elution with an aqueous buffer sterilized solution having a pH of about 5.5 to 7.5.
Claim 20
Independent Claim
187 words
A method of separating a virus from an aqueous medium containing said virus by liquid exclusion chromatography on a solid porous support wherein said support is silica gel or glass in the form of 40 to 200 microns particles having an average pore diameter of 5 to 200 nm, comprising subjecting said support to the following successive steps: (a) contact with an aqueous solution of a non-proteinic polymer selected from the group consisting of polyethylene glycol, polypropylene glycol, polybutylene glycol, polyvinyl alcohol, polyvinyl pyrrolidone and (polyethylene-polypropylene) glycol polymer, said polymer having a molecular weight of 5,000 to 30,000 and present at a concentration of 0.5 to 5 grams per liter; (b) contact with an aqueous solution containing 0.2 to 20% by weight of a protein whose molecular weight is lower than the molecular weight of the virus and does not exceed 100,000; (c) rinsing with an aqueous buffer sterilized solution having a pH of about 5.5 to 7.5; (d) contact with the aqueous medium containing the virus to be separated; and (e) elution with an aqueous buffer sterilized solution having a pH of about 5.5 to 7.5.
References Cited (Backward Citations)
US3549524A
Cited
US3476737A
Cited
US3467595A
Cited
US3701609A
Cited
US3926800A
Cited
US4123931A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-22 | 1983-10-22 | 1984-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-22 | 1987-10-22 | 1988-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-22 | 1991-10-22 | 1992-04-22 | $7700.00 | Window Closed / Lapsed |