Patent US4182695A successfully refreshed from Google Patents.
US4182695A
Expired / Lapsed
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Polyamide-fixed biologically active protein
Assignee: Roche Diagnostics GmbH
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Filing: 1978-02-16
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Granted: 1980-01-08
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Expires: 1998-02-16
Abstract
Abstract Biologically active protein compositions comprising protein or a protein substrate bound onto a polyamide of the general formula: ##STR1## in which R and R 1 , which may be the same or different, are polyamide residues bound onto the amido group, R 2 is the residue of a compound which can be condensed with formaldehyde and which contains at least one further reactive group, R 3 is the residue of a bi- or poly-functional protein reagent, n is 0 or 1, and P is a biologically active protein; these compositions are obtainable by the reaction of a polyamides with amounts, equimolar to one another, of formaldehyde and of a compound condensable with formaldehyde, in a solvent for polyamides, reacting the product thereby obtained with a bi- or poly-functional protein reagent and coupling the resulting reaction product with the biologically active protein or protein substrate.
Inventors
- 👤 Jurgen Horn
- 👤 Winfried Albert
- 👤 Hans-Georg Batz
- 👤 Michael Nelbock-Hochstetter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:10
CPC Classifications
C; C08; C08G; C08G69/00; C08G69/48; C08G69/50; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/089; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
157 words
Biologically active protein composition comprising protein or a protein substrate bound onto a polyamide, of the formula ##STR4## in which R and R 1 , which may be the same or different, are polyamide residues bound onto the amido group, R 2 is the residue of a compound which can be condensed with formaldehyde and which contains at least one further reactive group, R 3 is the residue of a bi- or poly-functional protein reagent, n is 0 or 1, and P is a biologically active protein wherein said composition is obtained by the reaction of polyamide with equal moles of formaldehyde and a compound which can be condensed with formaldehyde in a solvent for polyamides, and reacting the resulting product with a bi- or poly-functional protein reagent selected from dialdehydes, dihydroxysuccinimide esters, diacetals, bis-maleinimides, bifunctional imino esters, diepoxides and dicarboxylic acid chlorides, and then coupling the resulting product with a biologically active protein or protein substrate.
Claim 2
Dependent Claim
157 words
Biologically active protein composition comprising protein or a protein substrate bound onto a polyamide, of the formula ##STR4## in which R and R 1 , which may be the same or different, are polyamide residues bound onto the amido group, R 2 is the residue of a compound which can be condensed with formaldehyde and which contains at least one further reactive group, R 3 is the residue of a bi- or poly-functional protein reagent, n is 0 or 1, and P is a biologically active protein wherein said composition is obtained by the reaction of polyamide with equal moles of formaldehyde and a compound which can be condensed with formaldehyde in a solvent for polyamides, and reacting the resulting product with a bi- or poly-functional protein reagent selected from dialdehydes, dihydroxysuccinimide esters, diacetals, bis-maleinimides, bifunctional imino esters, diepoxides and dicarboxylic acid chlorides, and then coupling the resulting product with a biologically active protein or protein substrate.
Claim 3
Dependent Claim
15 words
Biologically active protein composition as claimed in claim 1 wherein R is a polycaprolactam residue.
Claim 4
Dependent Claim
24 words
Biologically active protein composition as claimed in claim 1 wherein R is a residue of a polyamide selected from 6,6-polyamide, 6,10-polyamide, 11-polyamide, or 12-polyamide.
Claim 5
Dependent Claim
16 words
Biologically active protein composition as claimed in claim 1 wherein R 1 is a polycaprolactam residue.
Claim 6
Dependent Claim
25 words
Biologically active protein composition as claimed in claim 1 wherein R 1 is a residue of a polyamide selected from 6,6-polyamide, 6,10-polyamide, 11-polyamide, or 12-polyamide.
Claim 7
Dependent Claim
18 words
Biologically active protein composition as claimed in claim 1 wherein R 2 is derived from phenol or aniline.
Claim 8
Dependent Claim
18 words
Biologically active protein composition as claimed in claim 1 wherein R 2 is derived from urea or thiourea.
Claim 9
Dependent Claim
16 words
Biologically active protein composition as claimed in claim 1 wherein R 2 is derived from melamine.
Claim 10
Dependent Claim
16 words
Biologically active protein composition as claimed in claim 1 wherein R 2 is derived from diaminotriazine.
Claim 11
Dependent Claim
27 words
Biologically active protein composition as claimed in claim 1 wherein R 2 is derived from aliphatic, aromatic or araliphatic diamine containing from 2 to 14 carbon atoms.
Claim 12
Dependent Claim
16 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from glutardialdehyde.
Claim 13
Dependent Claim
18 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a dihydroxysuccinimide ester.
Claim 14
Dependent Claim
17 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a diacetal.
Claim 15
Dependent Claim
16 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from bis-maleinimide.
Claim 16
Dependent Claim
19 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a bi-functional imino ester.
Claim 17
Dependent Claim
17 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a diepoxide.
Claim 18
Dependent Claim
19 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a dicarboxylic acid chloride.
Claim 19
Dependent Claim
27 words
Biologically active protein composition as claimed in claim 1 wherein R 3 is derived from a copolymer of acrylamide/methacrylamide and acrylic acid succinimide ester/methacrylic acid succinimide ester.
Claim 20
Dependent Claim
154 words
Process for fixing a biologically active protein composition comprising a protein or a protein substrate onto a polyamide which process comprises reacting the polyamide, in the presence of a solvent for polyamides, with amounts, equimolar to one another, of formaldehyde and of a compound which can be condensed with formaldehyde and contains at least one further reactive group, to result in the formation of a polyamide derivative of the formula ##STR5## wherein R and R 1 , which are the same or different, are polyamide residues bound onto the amido group, and R 2 is the residue of a compound which can be condensed with formaldehyde which contains at least one further reactive group, then reacting said polyamide derivative with a bi- or poly-functional protein reagent, and thereafter bringing the resulting reaction product together with a biologically active protein or protein substrate, in aqueous solution, to result in binding thereof to said reaction product.
Claim 21
Dependent Claim
154 words
Process for fixing a biologically active protein composition comprising a protein or a protein substrate onto a polyamide which process comprises reacting the polyamide, in the presence of a solvent for polyamides, with amounts, equimolar to one another, of formaldehyde and of a compound which can be condensed with formaldehyde and contains at least one further reactive group, to result in the formation of a polyamide derivative of the formula ##STR5## wherein R and R 1 , which are the same or different, are polyamide residues bound onto the amido group, and R 2 is the residue of a compound which can be condensed with formaldehyde which contains at least one further reactive group, then reacting said polyamide derivative with a bi- or poly-functional protein reagent, and thereafter bringing the resulting reaction product together with a biologically active protein or protein substrate, in aqueous solution, to result in binding thereof to said reaction product.
Claim 22
Dependent Claim
24 words
Process as claimed in claim 19 wherein the reaction with the formaldehyde is carried out in at least 50% formic acid or acetic acid.
Claim 23
Dependent Claim
25 words
Process as claimed in claim 19 wherein the polyamide is used in the form of a shaped body of which only the surface is dissolved.
Claim 24
Dependent Claim
22 words
Process as claimed in claim 19 wherein the reaction is carried by depositing the said polyamide derivative on a solid carrier surface.
Claim 25
Dependent Claim
25 words
Process as claimed in claim 21 wherein said shaped body is a solid carrier material the surface of which is coated with finely divided polyamide.
Claim 26
Dependent Claim
21 words
Process as claimed in claim 19 wherein said active protein or protein substrate is bound by reaction with nitrite and diazotization.
Claim 27
Dependent Claim
23 words
Process as claimed in claim 19 wherein said active protein or protein substrate is bound by means of phosgene, thiophosgene or cyanogen halide.
Claim 28
Dependent Claim
13 words
Process as claimed in claim 19 wherein said protein is a cross-linked protein.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-01-07 | 1983-07-09 | 1984-01-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-01-07 | 1987-07-09 | 1988-01-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-01-07 | 1991-07-09 | 1992-01-08 | $7700.00 | Window Closed / Lapsed |