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US4177107A Expired / Lapsed US ⏱️ 0.0 years remaining

Process for producing composition containing insolubilized enzyme and/or insolubilized bacterial cells

Assignee: Japan Atomic Energy Agency
•
Filing: 1977-01-28
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Granted: 1979-12-04
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Expires: 1997-01-28
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Abstract

Abstract A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer.

Inventors

  • 👤 Minoru Kumakura
  • 👤 Masaru Yoshida
  • 👤 Isao Kaetsu

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 15:49
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 214 words
A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer selected from the group consisting of a compound having the formula: ##STR10## wherein X is H or methyl, R 1 is --CH 2 -- n wherein n is an integer of 1 to 6, ##STR11## CH 2 --CH 2 --O-- m wherein m is an integer of 2 or more, or ##STR12## wherein l is an integer of 2 or more, and when R 1 is --CH 2 -- n or ##STR13## R 2 is --OH, --OCH 3 or ##STR14## wherein X is as defined above, when R 1 is --CH 2 --CH 2 --O m or ##STR15## R 2 is hydrogen, methyl, or ##STR16## wherein X is as defined above; or a compound having the formula: ##STR17## wherein X is as defined above; a compound having the formula ##STR18## wherein R is ethyl, n-propyl or isopropyl and X is as defined above; and mixtures thereof, and (ii) the step of irradiating the mixtures by means of an ionizing radiation at a temperature of less than -10° C.
Claim 2
Dependent Claim 214 words
A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer selected from the group consisting of a compound having the formula: ##STR10## wherein X is H or methyl, R 1 is --CH 2 -- n wherein n is an integer of 1 to 6, ##STR11## CH 2 --CH 2 --O-- m wherein m is an integer of 2 or more, or ##STR12## wherein l is an integer of 2 or more, and when R 1 is --CH 2 -- n or ##STR13## R 2 is --OH, --OCH 3 or ##STR14## wherein X is as defined above, when R 1 is --CH 2 --CH 2 --O m or ##STR15## R 2 is hydrogen, methyl, or ##STR16## wherein X is as defined above; or a compound having the formula: ##STR17## wherein X is as defined above; a compound having the formula ##STR18## wherein R is ethyl, n-propyl or isopropyl and X is as defined above; and mixtures thereof, and (ii) the step of irradiating the mixtures by means of an ionizing radiation at a temperature of less than -10° C.
Claim 3
Dependent Claim 26 words
The process as defined in claim 1, wherein the irradiation is carried out at a temperature in the range of from -80° C. to -25° C.
Claim 4
Dependent Claim 59 words
The process as defined in claim 1, wherein the monomer is selected from the group consisting of hydroxyethyl acrylate, hydroxypropyl methacrylate, glycidylacrylate, glycidylmethacrylate, tetraethyleneglycol methacrylate, tetraethyleneglycol acrylate, diethyleneglycol dimethacrylate, diethyleneglycol acrylate, polyethyleneglycol dimethacrylate, polyethyleneglycol diacrylate, neopentylglycol dimethacrylate, neopentylglycol diacrylate, trimethylol propane trimethacrylate, trimethylolpropane triacrylate, butanediol dimethacrylate, butanediol diacrylate, hexanediol dimethacrylate, hexanediol diacrylate, ethyleneglycol dimethacrylate, ethyleneglycol diacrylate and mixtures thereof.
Claim 5
Dependent Claim 34 words
The process as defined in claim 1, wherein the adsorbent is selected from the group consisting of molecular sieves, activated carbon, Kanuma soil, porous argil, porous ion-exchange resin, particulate calcium sulfate and mixtures thereof.

References Cited (Backward Citations)

US3577512A Cited
US3860490A Cited
US3962038A Cited
US3871964A Cited
US3859169A Cited
US3933587A Cited
US4025391A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1982-12-03 1983-06-04 1983-12-04 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1986-12-03 1987-06-04 1987-12-04 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1990-12-03 1991-06-04 1991-12-04 $7700.00 Window Closed / Lapsed

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