US3933587A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for production of immobilized enzyme
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1973-11-23
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Granted: 1976-01-20
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Expires: 1993-11-23
Abstract
Abstract Immobilized enzymes are produced by adding radioresistant enzymes to 5-25% aqueous solutions of polyvinyl type high molecular compounds and irradiating the enzyme-containing solutions with a high dosage of ionizing radiant rays in an inert atomsphere or under a decreased pressure for thereby gelling said solutions.
Inventors
- 👤 Hidekatsu Maeda
- 👤 Aizo Yamauchi
- 👤 Hideo Suzuki
- 👤 Akira Kamibayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:51
CPC Classifications
A; A61; A61K; A61K41/00; A61K41/0023; C; C12; C12N; C12N11/00; C12N11/02; C12N11/04
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
51 words
A method for the production of an immobilized enzyme, which comprises adding a radioresistant enzyme to the aqueous solution of a polyvinyl type high molecular compound selected from the group consisting of polyvinyl alcohol and polyvinyl pyrrolidone and irradiating the resultant mixture with ionizing radiant rays for thereby gelling the mixture.
Claim 2
Independent Claim
51 words
A method for the production of an immobilized enzyme, which comprises adding a radioresistant enzyme to the aqueous solution of a polyvinyl type high molecular compound selected from the group consisting of polyvinyl alcohol and polyvinyl pyrrolidone and irradiating the resultant mixture with ionizing radiant rays for thereby gelling the mixture.
Claim 3
Dependent Claim
28 words
A method according to claim 1, wherein the radioresistant enzyme is one member selected from the group consisting of glucoamylase, invertase, beta-galactosidase, glucose oxidase, glucose isomerase and catalase.
Claim 4
Dependent Claim
36 words
A method according to claim 1, wherein the amount of the enzyme to be added to the aqueous solution of polyvinyl type high molecular compound is in the range of from 0.001 to 5% by weight.
Claim 5
Dependent Claim
29 words
A method according to claim 1, wherein the ionizing radiant rays are one or more selected from the group consisting of beta rays, gamma rays or accelerated electron rays.
Claim 6
Dependent Claim
24 words
A method according to claim 4, wherein the dosage of the radiant rays is in the range of from 1 M.rad to 20 M.rads.
References Cited (Backward Citations)
DE1955638A1
Cited
US3788950A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-01-19 | 1979-07-21 | 1980-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-01-19 | 1983-07-21 | 1984-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-01-19 | 1987-07-21 | 1988-01-20 | $7700.00 | Window Closed / Lapsed |