Patent US4164464A successfully refreshed from Google Patents.
US4164464A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Sample concentrator
Assignee: Instrumentation Specialties Co
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Filing: 1978-04-14
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Granted: 1979-08-14
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Expires: 1998-04-14
Abstract
Abstract To concentrate a material by separating it from a diluting medium, the combination of the material and medium is placed in one of two wells in the bottom of a plastic sample concentrator cell, with each well being closed at its bottom end by a different cellophane membrane in contact with a buffer solution in a different one of two buffer compartments. A buffer solution also connects the combination of material and medium in one well and the cellophane bottom of the other well within the sample concentration cell through a recess in the bottom of the sample concentration cell. A potential is applied across the two buffer compartments to cause the material to migrate by electrophoresis from the medium in one well, through the buffer in the sample concentrating cell and into the other well, where it is concentrated against the cellophane membrane for easy removal by pipetting.
Inventors
- 👤 William B. Allington, deceased
- 👤 James W. Nelson
- 👤 Arthur L. Cordry
- 👤 Gail A. McCullough
- 👤 Don E. Mitchell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:57
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/4473; B; B01; B01D; B01D57/00; B01D57/02; G01N1/00; G01N1/28; G01N1/34; G01N1/40; G01N1/4005; G01N2001/4016; G01N2001/4038
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
110 words
Apparatus for separating at least one molecular species from a sample, comprising: a sample concentrating cell; said sample concentrating cell having first and second sections; said first section including a member adapted to receive a sample; said second section including a membrane having pores sufficiently small to permit the membrane to hold said one molecular species and being adapted to receive at least one of the separated molecular species; wall means for at least partly confining said first and second sections and adapted to support a buffer solution; means for establishing an electrical potential between the side of the sample and the side of said membrane farthest from each other.
Claim 2
Dependent Claim
110 words
Apparatus for separating at least one molecular species from a sample, comprising: a sample concentrating cell; said sample concentrating cell having first and second sections; said first section including a member adapted to receive a sample; said second section including a membrane having pores sufficiently small to permit the membrane to hold said one molecular species and being adapted to receive at least one of the separated molecular species; wall means for at least partly confining said first and second sections and adapted to support a buffer solution; means for establishing an electrical potential between the side of the sample and the side of said membrane farthest from each other.
Claim 3
Dependent Claim
13 words
Apparatus according to claim 1 in which said member includes a second membrane.
Claim 4
Dependent Claim
15 words
Apparatus according to claim 2 in which said first and second membranes are cellophane membranes.
Claim 5
Dependent Claim
87 words
Apparatus according to claim 3 in which: said sample concentrating cell includes top, bottom and side surfaces; said first section includes internal walls defining a first opening; said first-mentioned membrane closes said first opening at said bottom surface; said second section includes internal walls defining a second opening; said second membrane closes said second opening at said bottom, whereby said sample is placed in one opening, a buffer solution communicates between the first and second openings and the separated molecular species is received in said second opening.
Claim 6
Dependent Claim
100 words
Apparatus according to claim 4 further comprising: an electrophoretic cell; said electrophoretic cell including walls defining first and second buffer compartments; one of said walls defining said first and second buffer compartments comprising a separating wall between said first and second buffer compartments; said first and second compartments including means for receiving different electrodes, whereby a potential difference may be established between said first and second buffer compartments; and said sample concentrating cell including means for mounting said sample concentrating cell to said separating wall with one membrane within said first compartment and the other membrane within said second compartment.
Claim 7
Dependent Claim
34 words
Apparatus according to claim 5 in which said mounting means comprises internal walls defining a groove in the outside surface of said bottom of said sample concentrating cell adapted to receive said separating wall.
Claim 8
Dependent Claim
24 words
Apparatus according to claim 6 further including a recess in the inner bottom of said sample concentrating cell connecting said first and second sections.
Claim 9
Dependent Claim
39 words
Apparatus according to claim 7 in which said internal walls defining a groove include projections whereby said internal walls are spaced from said separating wall to avoid capillary motion of buffer solution to the top of the separating wall.
Claim 10
Dependent Claim
18 words
Apparatus according to claim 7 further including removable insert means for reducing the volume of said second opening.
Claim 11
Dependent Claim
33 words
Apparatus according to claim 7 further including means for permitting a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 12
Dependent Claim
90 words
Apparatus according to claim 2 in which: said sample concentrating cell includes top, bottom and side surfaces; said first section includes internal walls defining a first opening; said first mentioned membrane closes said first opening at said bottom surface; said second section includes internal walls defining a second opening; said second membrane closes said second opening at said bottom surface, whereby said sample is placed in said first opening, a buffer solution communicates between the first and second openings and the separated molecular species is received in the second opening.
Claim 13
Dependent Claim
100 words
Apparatus according to claim 11 further comprising: an electrophoretic cell; said electrophoretic cell including walls defining first and second buffer compartments; one of said walls defining said first and second buffer compartments comprising a separating wall between said first and second buffer compartments; said first and second compartments including means for receiving different electrodes, whereby a potential difference may be established between said first and second buffer compartments; and said sample concentrating cell including means for mounting said sample concentrating cell to said separating wall with one membrane within said first compartment and the other membrane within said second compartment.
Claim 14
Dependent Claim
26 words
Apparatus according to claim 12 in which said mounting means comprises a groove in the outside surface of said bottom wall of said sample concentrating cell.
Claim 15
Dependent Claim
24 words
Apparatus according to claim 13 further including a recess in the inner bottom of said sample concentrating cell connecting said first and second sections.
Claim 16
Dependent Claim
33 words
Apparatus according to claim 14 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 17
Dependent Claim
100 words
Apparatus according to claim 2 further comprising: an electrophoretic cell; said electrophoretic cell including walls defining first and second buffer compartments; one of said walls defining said first and second buffer compartments comprising a separating wall between said first and second buffer compartments; said first and second compartments including means for receiving different electrodes, whereby a potential difference may be established between said first and second buffer compartments; and said sample concentrating cell including means for mounting said sample concentrating cell to said separating wall with one membrane within said first compartment and the other membrane within said second compartment.
Claim 18
Dependent Claim
26 words
Apparatus according to claim 16 in which said mounting means comprises a groove in the outside surface of said bottom wall of said sample concentrating cell.
Claim 19
Dependent Claim
24 words
Apparatus according to claim 15 further including a recess in the inner bottom of said sample concentrating cell connecting said first and second sections.
Claim 20
Dependent Claim
33 words
Apparatus according to claim 18 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 21
Dependent Claim
24 words
Apparatus according to claim 5 further including a recess in the inner bottom of said sample concentrating cell connecting said first and second sections.
Claim 22
Dependent Claim
33 words
Apparatus according to claim 20 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 23
Dependent Claim
24 words
Apparatus according to claim 12 further including a recess in the inner bottom of said sample concentrating cell connecting said first and second sections.
Claim 24
Dependent Claim
33 words
Apparatus according to claim 22 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 25
Dependent Claim
24 words
Apparatus according to claim 16 further including a recess in the inner bottom of said sample concentrating cell connecting said member and first-mentioned membrane.
Claim 26
Dependent Claim
33 words
Apparatus according to claim 24 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 27
Dependent Claim
33 words
Apparatus according to claim 1 further including means for enabling a continuous flow of said sample through said first location, whereby said first molecular species may be removed from a continuously flowing stream.
Claim 28
Dependent Claim
13 words
Apparatus according to claim 26 in which said member includes a second membrane.
Claim 29
Dependent Claim
89 words
Apparatus according to claim 27 in which: said sample concentrating cell includes top, bottom and side surfaces; said first section includes internal walls defining a first opening; said first-mentioned membrane closes said first opening at said bottom surface; said second section includes internal walls defining a second opening; said second membrane closes said second opening at said bottom surface, whereby said sample is placed in said first opening, a buffer solution communicates between the first and second openings and the separated molecular species is received in the second opening.
Claim 30
Dependent Claim
75 words
Apparatus according to claim 26 further comprising: an electrophoretic cell; said electrophoretic cell including walls defining first and second buffer compartments; one of said walls defining said first and second buffer compartments comprising a separating wall between said first and second buffer compartments; said first and second compartments including means for mounting said sample concentrating cell to said separating wall with one membrane within said first compartment and the other membrane within said second compartment.
References Cited (Backward Citations)
US3759773A
Cited
US3715295A
Cited
US3951776A
Cited
US3829375A
Cited
US3755121A
Cited
US3902987A
Cited
US3932263A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-08-13 | 1983-02-12 | 1983-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-08-13 | 1987-02-12 | 1987-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-08-13 | 1991-02-12 | 1991-08-14 | $7700.00 | Window Closed / Lapsed |