US3715295A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Disposable electrophoresis unit
Assignee: Tlc Corp
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Filing: 1971-09-02
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Granted: 1973-02-06
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Expires: 1991-09-02
Abstract
Abstract This invention relates to an expendable electrophoresis test unit for particle separation in a buffered system using direct current. The buffer is in semi-solid form which can be prepackaged with the unit, or dispensed from a tube at the time of use. The semi-solid buffer is prepared by mixing a liquid buffer solution with a thickening agent, preferably fumed silica. The unit itself is made of inexpensive disposable materials.
Inventors
- 👤 P Tocci
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 13:28
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
115 words
A disposable electrophoresis test unit comprising, a tank having integrally formed means defining a pair of spacEd wells for holding a buffer in semi-solid form therein; a buffer in substantially irreversible semi-solid form placed in each of said spaced wells; a support media positioned at the top of the tank and in electrical contact with said semi-solid buffer in the wells; and means including electrodes within said wells for making electrical contact with said buffer, and connected to a suitable source of direct current to provide passage of current through said buffer and support media in order to move particles according to their electrical charge and permit charged particle migration for particle group identification thereof.
Claim 2
Dependent Claim
115 words
A disposable electrophoresis test unit comprising, a tank having integrally formed means defining a pair of spacEd wells for holding a buffer in semi-solid form therein; a buffer in substantially irreversible semi-solid form placed in each of said spaced wells; a support media positioned at the top of the tank and in electrical contact with said semi-solid buffer in the wells; and means including electrodes within said wells for making electrical contact with said buffer, and connected to a suitable source of direct current to provide passage of current through said buffer and support media in order to move particles according to their electrical charge and permit charged particle migration for particle group identification thereof.
Claim 3
Dependent Claim
11 words
The electrophoresis test unit of claim 1 including a closure therefor.
Claim 4
Dependent Claim
18 words
The electrophoresis test unit of claim 2 wherein said closure is a wrapper of plastic or metal foil.
Claim 5
Dependent Claim
60 words
The electrophoresis test unit of claim 2 wherein the top of said tank is rectangular in shape and said closure is a lid hinged to said tank along an edge thereof, and a raised rib along the remaining edges of said tank is adapted frictionally to engage said lid along its unhinged edges to close said tank to the atmosphere.
Claim 6
Dependent Claim
31 words
The electrophoresis test unit of claim 1 wherein said semi-solid buffer is a suspension of a buffer solution in particles of a thickening agent forming a semisolid substantially irreversible by heat.
Claim 7
Dependent Claim
14 words
The electrophoresis unit of claim 5 in which said thickening agent is fumed silica.
Claim 8
Dependent Claim
16 words
The electrophoresis test unit of claim 1 wherein said support media is a thin layer plate.
Claim 9
Dependent Claim
14 words
The electrophoresis test unit of claim 1 wherein said chamber is made of plastic.
Claim 10
Dependent Claim
72 words
The process of separating materials by electrophoresis using the electrophoresis unit of claim 1 which comprises depositing a test sample on said buffered support media which is in electrical contact with the semi-solid buffer in the wells of said unit, and applying direct current through the components of the system for a predetermined length of time to cause separation of the components of said materials by differential migration in the electric field.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-02-06 | 1976-08-07 | 1977-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-02-06 | 1980-08-07 | 1981-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-02-06 | 1984-08-07 | 1985-02-06 | $7700.00 | Window Closed / Lapsed |