Patent US4164444A successfully refreshed from Google Patents.
US4164444A
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Method for preparing adenosine triphosphate
Assignee: Massachusetts Institute of Technology
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Filing: 1977-01-31
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Granted: 1979-08-14
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Expires: 1997-01-31
Abstract
Abstract Acyl phosphate salts suitable for use as phosphate donors in the production of cofactors, such as adenosine triphosphate, are prepared by acylation of phosphoric acid with a ketene, followed by reaction with ammonia or an amine. The reaction product, e.g. diammonium acetyl phosphate, can normally be used directly in enzymic production of cofactors without the necessity of prior conversion to the free acid or another salt, such as the sodium salt. The acyl phosphate salts are produced in high yields, and the method allows production of cofactors for use in biosynthesis from readily available materials.
Inventors
- 👤 George M. Whitesides
- 👤 Patricia E. Garrett
- 👤 Merrell G. Siegel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 18:23
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/32; C07; C07F; C07F9/00; C07F9/02; C07F9/06; C07F9/08; C07F9/09; C07F9/095; C07F9/096
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
47 words
A method of preparing adenosine triphosphate in an enzyme-catalyzed reaction, said method comprising the step of reacting adenosine diphosphate in the presence of a phosphotransferase with an acetyl phosphate salt in which the cation is derived from the group of ammonia and primary, secondary and tertiary amines.
Claim 2
Independent Claim
47 words
A method of preparing adenosine triphosphate in an enzyme-catalyzed reaction, said method comprising the step of reacting adenosine diphosphate in the presence of a phosphotransferase with an acetyl phosphate salt in which the cation is derived from the group of ammonia and primary, secondary and tertiary amines.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein the acetyl phosphate salt is diammonium acetyl phosphate.
Claim 4
Dependent Claim
15 words
The method of claim 2, in which the acetyl phosphate salt is dianilinium acetyl phosphate.
Claim 5
Dependent Claim
26 words
The method of claim 1, wherein the reaction is carried out by mixing adenosine diphosphate and the acetyl phosphate salt in the presence of acetate kinase.
Claim 6
Dependent Claim
35 words
The method of claim 4, wherein said adenosine diphosphate and said acetyl phosphate salt are mixed with from about 700 to about 2000 units of acetate kinase per mole of acetyl phosphate salt per day.
Claim 7
Dependent Claim
14 words
The method of claim 5, wherein said acetyl phosphate salt is diammonium acetyl phosphate.
Claim 8
Dependent Claim
38 words
The method of claim 4, wherein said acetyl phosphate salt is diammonium acetyl phosphate and said acetate kinase is present in an amount from about 1200 to about 1600 units per mole of diammonium acetyl phosphate per day.
Claim 9
Dependent Claim
17 words
The method of claim 1, wherein said reaction is carried out in the presence of adenylate kinase.
Claim 10
Dependent Claim
34 words
The method of claim 5, wherein said adenosine diphosphate and said acetyl phosphate salt are mixed with from about 200 to about 1400 units of adenylate kinase per mole of acetyl phosphate per day.
Claim 11
Dependent Claim
27 words
The method of claim 8, further comprising producing at least a portion of said adenosine diphosphate by reacting adenosine monophosphate in the presence of said adenylate kinase.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-08-13 | 1983-02-12 | 1983-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-08-13 | 1987-02-12 | 1987-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-08-13 | 1991-02-12 | 1991-08-14 | $7700.00 | Window Closed / Lapsed |