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US4158703A
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T3 uptake test employing covalently bound bovine serum albumin
Assignee: Beckman Coulter Inc
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Filing: 1978-02-13
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Granted: 1979-06-19
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Expires: 1998-02-13
Abstract
Abstract An improved T 3 uptake test procedure characterized in that the separating agent employed therein comprises bovine serum albumin covalently coupled to a derivatized polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR1## WHEREIN N IS AN INTEGER FROM 1 TO 6 AND WHEREIN E IS AN INTEGER FROM 1 TO 2. A separating agent comprising bovine serum albumin covalently coupled to a derivatived polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR2## WHEREIN N IS AN INTEGER FROM 1 TO 6 AND WHEREIN E IS AN INTEGER FROM 1 TO 2.
Inventors
- 👤 Alan J. Polito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/826; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
104 words
An improved T 3 uptake test procedure of the type wherein: (a) a solution is contacted with labeled T 3 and a separating agent; (b) serum bound labeled T 3 and separating agent bound labeled T 3 are separated into two fractions; (c) at least one of said fractions is measured; wherein the improvement comprises contacting said solution with a separating agent comprising bovine serum albumin covalently coupled to a derivatized polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR11## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 2
Dependent Claim
104 words
An improved T 3 uptake test procedure of the type wherein: (a) a solution is contacted with labeled T 3 and a separating agent; (b) serum bound labeled T 3 and separating agent bound labeled T 3 are separated into two fractions; (c) at least one of said fractions is measured; wherein the improvement comprises contacting said solution with a separating agent comprising bovine serum albumin covalently coupled to a derivatized polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR11## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 3
Dependent Claim
22 words
The procedure of claim 1 wherein said separating agent has a formula ##STR12## wherein m is an integer from 1 to 12.
Claim 4
Dependent Claim
43 words
The procedure of claim 2 wherein said polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof; wherein m is an integer from 4 to 6; and wherein n is an integer from 4 to 6.
Claim 5
Dependent Claim
10 words
The procedure of claim 3 wherein said label is radioactive.
Claim 6
Dependent Claim
11 words
The procedure of claim 4 wherein said label is 125 I.
Claim 7
Dependent Claim
10 words
The procedure of claim 1 wherein said label is radioactive.
Claim 8
Dependent Claim
11 words
The procedure of claim 6 wherein said label is 125 I.
Claim 9
Dependent Claim
28 words
The procedure of claim 1 wherein said derivatized polysaccharide matrix is a finely divided, derivatized polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns.
Claim 10
Dependent Claim
22 words
The procedure of claim 8 wherein said separating agent has a formula ##STR13## wherein m is an integer from 1 to 12.
Claim 11
Dependent Claim
45 words
The procedure of claim 9 wherein said finely divided, polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof; wherein m is an integer from 4 to 6 and wherein n is an integer from 4 to 6.
Claim 12
Dependent Claim
34 words
The procedure of claim 10 wherein said finely divided polysaccharide matrix is selected from the group consisting of cellulosic polymers and derivatives thereof having an average wet maximum dimension of 10 to 15 microns.
Claim 13
Dependent Claim
10 words
The procedure of claim 11 wherein said label is radioactive.
Claim 14
Dependent Claim
11 words
The procedure of claim 12 wherein said label is 125 I.
Claim 15
Dependent Claim
10 words
The procedure of claim 8 wherein said label is radioactive.
Claim 16
Dependent Claim
11 words
The procedure of claim 14 wherein said label is 125 I.
Claim 17
Dependent Claim
45 words
A separating agent comprising bovine serum albumin covalently coupled to a derivatized polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR14## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 18
Dependent Claim
45 words
A separating agent comprising bovine serum albumin covalently coupled to a derivatized polysaccharide matrix by a bifunctional coupling agent selected from a group consisting of ##STR14## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 19
Dependent Claim
19 words
The separating agent of claim 16 having a formula ##STR15## wherein m is an integer from 1 to 12.
Claim 20
Dependent Claim
44 words
The separating agent of claim 17 wherein said polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof; wherein m is an integer from 4 to 6; and wherein n is an integer from 4 to 6.
Claim 21
Dependent Claim
29 words
The separating agent of claim 16 wherein said derivatized polysaccharide matrix is a finely divided, derivatized polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns.
Claim 22
Dependent Claim
23 words
The separating agent of claim 19 having a formula finely divided, polysaccharide matrix- ##STR16## wherein m is an integer from 1 to 12.
Claim 23
Dependent Claim
46 words
The separating agent of claim 20 wherein said finely divided, polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof; wherein m is an integer from 4 to 6 and wherein n is an integer from 4 to 6.
Claim 24
Dependent Claim
35 words
The separating agent of claim 21 wherein said finely divided, polysaccharide matrix is selected from the group consisting of cellulosic polymers and derivatives thereof having an average wet maximum dimension of 10 to 15 microns.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-06-18 | 1982-12-18 | 1983-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-06-18 | 1986-12-18 | 1987-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-06-18 | 1990-12-18 | 1991-06-19 | $7700.00 | Window Closed / Lapsed |