US4081245A
Expired / Lapsed
US
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Immunoassay procedure employing novel immunochemical composites
Assignee: Beckman Coulter Inc
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Filing: 1976-05-03
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Granted: 1978-03-28
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Expires: 1996-05-03
Abstract
Abstract A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a composite comprising a derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent, wherein the improvement comprises selecting said bifunctional coupling agent from a group comprising ##STR1## WHEREIN N IS AN INTEGER FROM 1 TO 6 AND WHEREIN E IS AN INTEGER FROM 1 TO 2.
Inventors
- 👤 Alan J. Polito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 07:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/826; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
73 words
A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a immunochemical composite comprising a derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent, wherein the improvement comprises selecting said bifunctional coupling agent from a group consisting of ##STR14## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 2
Independent Claim
73 words
A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a immunochemical composite comprising a derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent, wherein the improvement comprises selecting said bifunctional coupling agent from a group consisting of ##STR14## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein said immunochemical composite has a formula ##STR15## wherein m is an integer from 1 to 12.
Claim 4
Dependent Claim
43 words
The method of claim 2 wherein said polysaccharide matrix is selected from the group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof, wherein m is an integer from 4 to 6, and wherein n is an integer from 4 to 6.
Claim 5
Dependent Claim
12 words
The method of claim 3 wherein said antibody is a secondary antibody.
Claim 6
Dependent Claim
13 words
The method of claim 4 wherein said immunoassay procedure is a radioimmunoassay procedure.
Claim 7
Dependent Claim
13 words
The method of claim 1 wherein said immunoassay procedure is a radioimmunoassay procedure.
Claim 8
Dependent Claim
65 words
An immunochemical composite for separating free from bound fractions in an immunoassay procedure of the type having a derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent, wherein the improvement comprises selecting said bifunctional coupling agent from a group consisting of ##STR16## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 9
Dependent Claim
65 words
An immunochemical composite for separating free from bound fractions in an immunoassay procedure of the type having a derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent, wherein the improvement comprises selecting said bifunctional coupling agent from a group consisting of ##STR16## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2.
Claim 10
Dependent Claim
20 words
The immunochemical composite of claim 7 having a formula ##STR17## ps wherein m is an integer from 1 to 12.
Claim 11
Dependent Claim
44 words
The immunochemical composite of claim 8 wherein said polysaccharide matrix is selected from the group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof, wherein m is an integer from 4 to 6 and wherein n is an integer from 4 to 6.
Claim 12
Dependent Claim
13 words
The immunochemical composite of claim 9 wherein said antibody is a secondary antibody.
References Cited (Backward Citations)
US3555143A
Cited
US3645852A
Cited
US3914183A
Cited
US3904478A
Cited
US3867366A
Cited
US3896217A
Cited
US3980765A
Cited
US3975511A
Cited
US3947352A
Cited
US4002532A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-27 | 1981-09-26 | 1982-03-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-27 | 1985-09-26 | 1986-03-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-27 | 1989-09-26 | 1990-03-28 | $7700.00 | Window Closed / Lapsed |