High specific activity labeled substances

Claim 2

A reagent for the determination of a component of the antigen-antibody reaction comprising (1) an antigen or antibody and (2) the other component covalently linked to an enzyme-spine material product having the formula ##STR66## wherein R is an organic group labeled with a radioisotope, fluorescent group, lysis-initiating compound, enzyme or other suitable marker material and depending on the coupling chemistry selected, linking moieties; T 1 and T 2 are individually selected from the group consisting of --CH 3 , ##STR67## --CH 2 OH, --CH 2 SH and --NH 2 ; X is selected from the group consisting of a divalent group of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, halogen-substituted phenylene, alkyl substituted phenylene, or aminated phenylene group; a is 0, 1 or 2; b is 2-a, with the proviso that there be at least two R groups in the product; and n is a number of from one to about 100,000.

Claim 3

The reagent of claim 1 wherein the marker material is the enzyme peroxidase.

Claim 4

The reagent of claim 2 wherein said peroxidase is horseradish peroxidase.

Claim 5

A reagent for the determination of a hapten being a protein-free substance that is not capable of stimulating antibody formation but which reacts with antibodies by means of a binding protein capable of reacting to bind said hapten specifically, comprising: (1) the coupling product of said hapten and an enzymespine material product having the formula ##STR68## where R is a group containing a radioisotope, fluorescent material, lysis-initiating compound, spine, or other suitable marker material lacking in linking atom and depending on the coupling chemistry selected, a linking moiety; T 1 and T 2 are individually selected from the group consisting of --CH 3 , ##STR69## CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent unit of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene, and aminated phenylene groups; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least two R groups in the product; and n is a number of from one to about 100,000; and (2) a binding protein capable of reacting to bind said hapten and said hapten-marker-spine material coupling product.

Claim 6

A reagent for the determination of a hapten being a protein-free substance that is not capable of stimulating antibody formation but which reacts with antibodies by means of a binding protein capable of reacting to bind said hapten specifically, comprising: (1) the coupling product of said hapten and an enzymespine material product having the formula ##STR68## where R is a group containing a radioisotope, fluorescent material, lysis-initiating compound, spine, or other suitable marker material lacking in linking atom and depending on the coupling chemistry selected, a linking moiety; T 1 and T 2 are individually selected from the group consisting of --CH 3 , ##STR69## CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent unit of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene, and aminated phenylene groups; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least two R groups in the product; and n is a number of from one to about 100,000; and (2) a binding protein capable of reacting to bind said hapten and said hapten-marker-spine material coupling product.

Claim 7

A reagent for the determination of a component of an antigenantibody reaction selected from the group consisting of an antigen and an antibody, comprising: (1) one of said components coupled to a carrier, (2) a substance having the same immunological properties as the component in (1) covalently linked to an enzyme-spine material product having the formula ##STR70## wherein R is an organic group labeled with a radioisotope, fluorescent material, lysis initiating material, enzyme, or other suitable marker material lacking a linking atom; and depending on the coupling chemistry selected, a linking moiety; T 1 and T 2 are individually selected from the group consisting of ##STR71## --CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent unit of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene, or aminated phenylene groups; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least two R groups in the product; and n is a number from one to about 100,000; and (3) the binding partner of the component to be determined if the component to be determined has the same immunological properties as the component in (1).

Claim 8

A reagent for the determination of a component of an antigenantibody reaction selected from the group consisting of an antigen and an antibody, comprising: (1) one of said components coupled to a carrier, (2) a substance having the same immunological properties as the component in (1) covalently linked to an enzyme-spine material product having the formula ##STR70## wherein R is an organic group labeled with a radioisotope, fluorescent material, lysis initiating material, enzyme, or other suitable marker material lacking a linking atom; and depending on the coupling chemistry selected, a linking moiety; T 1 and T 2 are individually selected from the group consisting of ##STR71## --CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent unit of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene, or aminated phenylene groups; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least two R groups in the product; and n is a number from one to about 100,000; and (3) the binding partner of the component to be determined if the component to be determined has the same immunological properties as the component in (1).

Claim 9

The reagent of claim 5, wherein said carrier is a water-spine insoluble, water-insusceptible, solid carrier.

Claim 10

A diagnostic tool for immunoassay comprising a marked-spine material product of the formula ##STR72## wherein R is an organic group labeled with a radioisotope, fluorescent group, lysis-initiating compound, enzyme or other suitable marker material lacking a linking atom; T 1 and T 2 are individually selected from the group consisting of --CH 3 , ##STR73## --CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent group of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene or aminated phenylene group; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least one R group in the product; and n is a number from 1 to about 100,000.

Claim 11

A diagnostic tool for immunoassay comprising a marked-spine material product of the formula ##STR72## wherein R is an organic group labeled with a radioisotope, fluorescent group, lysis-initiating compound, enzyme or other suitable marker material lacking a linking atom; T 1 and T 2 are individually selected from the group consisting of --CH 3 , ##STR73## --CH 2 OH, --CH 2 SH, and --NH 2 ; X is selected from the group consisting of a divalent group of the formula (CH 2 ) m wherein m is an integer from 0 to about 10, phenylene, hydroxyl-substituted phenylene, halogen-substituted phenylene, alkyl substituted phenylene or aminated phenylene group; a is 0, 1, or 2; b is 2-a, with the proviso that there be at least one R group in the product; and n is a number from 1 to about 100,000.