Radioimmunoassay of angiotensin and renin activity

Abstract

Abstract A PROCESS FOR ASSAYING THE QUANTITY OF ANGIOTENSIN AND RENIN EMPLOYING RADIOIMMUNOASSAY TECHNIQUES, COMPRISING THE STEPS OF COMBINING SOLUTIONS OF (1) ANTIBODY TO ANGIOTENSIN COUPLED TO POLY-L-LYSINE, (2) ANGIOTENSIN LABELED WITH RADIOACTIVITY, AND (3) VARYING KNOWN AMOUNTS OF UNLABELED ANGIOTENSIN, TO PRODUCE MIXTURES HAVING A PH OF ABOUT 7.0 TO 9.5, WHICH UPON INCUBATION FORM ANGIOTENSIN-ANTIBODY COMPLEXES. THE MIXTURES OF ANGIOTENSIN-ANTIBODY COMPLEXES (ANTIDOBY-BOUND ANTIOTENSIN) AND FREE ANGIOTENSIN ARE APPLIED EITHER TO PAPER STRIPS, COATED CHARCOAL, ION EXCHANGE RESINS OR RESIN SPONGES, AND SUBJECTED TO RADIOIMMUNOASSAY TECHNIQUES TO SEPARATE THE ANTIBODY-BOUND ANGIOTENSIN COMPLEXES FROM THE FREE ANGIOTENSIN. SOME OF THE FREE AND ANTIBODY-BOUND ANGIOTENSIN IS RADIOACTIVE; THE RATIO OF RADIOACTIVITY OF THE ANTIBODY-BOUND ANGIOTENSIN COMPLEX TO FREE ANGIOTENSIN IS PLOTTED AS A FUNCTION OF THE KNOWN CONVENTRATIONS OF ANGIOTENSIN IN EACH MIXTURE TO PREPARE A STANDARD CONCENTRATION GRAPH. THE PROCESS IS REPEATED EMPLOYING UNKNOWN AMOUNTS OF UNLABELED ANGIOTENSIN, WHICH MAY BE DERIVED FROM THE ACTION OF RENIN ON RENIN SUBSTRATE, AND THE CONCENTRATION OF ANGIOTENSIN IS READ FROM THE GRAPH.

Inventors

• 👤 Robert L Wolf

Bibliographic Details