Patent US4139604A successfully refreshed from Google Patents.
US4139604A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation technique for assays
Assignee: Becton Dickinson and Co
•
Filing: 1977-09-16
•
Granted: 1979-02-13
•
Expires: 1997-09-16
Abstract
Abstract In an assay wherein radiolabeled substance bound to a receptor is separated from unbound radiolabeled substance, such separation is effected by the use of a combination of a particulate adsorbent and a thickening agent to decrease adsorption time. The procedure can be employed, for example, in separating bound iron from free iron in a total or unsaturated iron binding capacity assay.
Inventors
- 👤 Sidney Gutcho
- 👤 Joan K. Givas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 14:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/538
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
78 words
In a liquid phase assay wherein bound labeled substance is separated from unbound labeled substance, the improvement comprising: adding to a solution containing bound labeled substance and unbound labeled substance a particulate adsorbent for the substance and a thickening agent different than the adsorbent which increases the viscosity of the solution to maintain the adsorbent in suspension in the solution; and separating solution containing bound labeled substance from the thickening agent and adsorbent containing adsorbed unbound labeled substance.
Claim 2
Dependent Claim
78 words
In a liquid phase assay wherein bound labeled substance is separated from unbound labeled substance, the improvement comprising: adding to a solution containing bound labeled substance and unbound labeled substance a particulate adsorbent for the substance and a thickening agent different than the adsorbent which increases the viscosity of the solution to maintain the adsorbent in suspension in the solution; and separating solution containing bound labeled substance from the thickening agent and adsorbent containing adsorbed unbound labeled substance.
Claim 3
Dependent Claim
11 words
The process of claim 1 wherein the assay is a radioassay.
Claim 4
Dependent Claim
13 words
The process of claim 2 wherein the assay is a radioassay for iron.
Claim 5
Dependent Claim
19 words
The process of claim 3 wherein the weight ratio of thickening agent to adsorbent is from 1:0.25 to 1:40.
Claim 6
Dependent Claim
14 words
The process of claim 4 wherein the particulate adsorbent is an ion exchange resin.
Claim 7
Dependent Claim
12 words
The process of claim 5 wherein the thickening agent is fumed silica.
Claim 8
Dependent Claim
28 words
The process of claim 2 wherein the thickening agent is selected from the group consisting of fumed silica, cellulose polymers; natural polymers, sorbitol, dextran, glycerin and propylene glycol.
Claim 9
Dependent Claim
19 words
The process of claim 2 wherein the weight ratio of suspending agent to adsorbent is from 1:0.25 to 1:40.
Claim 10
Dependent Claim
14 words
The process of claim 8 wherein the weight ratio is from 1:1 to 1:5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-12 | 1982-08-14 | 1983-02-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-12 | 1986-08-14 | 1987-02-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-12 | 1990-08-14 | 1991-02-13 | $7700.00 | Window Closed / Lapsed |