Patent US4128629A successfully refreshed from Google Patents.
US4128629A
Expired / Lapsed
US
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Extraction-free cortisol assay
Assignee: Corning Glass Works
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Filing: 1977-07-05
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Granted: 1978-12-05
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Expires: 1997-07-05
Abstract
Abstract Immobilization of active anti-cortisol antibodies onto negatively charged solid phase surfaces results in a shift of the antibody pH optimum to an acidic range thereby permitting effective yet minimal use of deblocking agents and an extraction-free immunoassay for cortisol.
Inventors
- 👤 Emmet W. Eldred
- 👤 Hubert J. P. Schoemaker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 10:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
130 words
An immunoassay for determining the concentration of cortisol in a serum sample, the assay comprising the steps of: (a) incubating the sample, labeled cortisol, and an effective amount of deblocking agent in an aqueous medium with a composite comprising anti-cortisol antibodies fixed onto the surfaces of a negatively charged support material, the incubation being at a pH ranging from about 4.0 to about 6.5 and being under conditions sufficient to result in the formation of immunochemical complexes on the composite, some of which complexes include labeled cortisol; (b) separating the composite from the incubation medium; (c) determining the amount of label on the separated composite or in the remaining incubation medium; and (d) relating the determination of step (c) to a standard to determine the cortisol concentration in the sample.
Claim 2
Dependent Claim
130 words
An immunoassay for determining the concentration of cortisol in a serum sample, the assay comprising the steps of: (a) incubating the sample, labeled cortisol, and an effective amount of deblocking agent in an aqueous medium with a composite comprising anti-cortisol antibodies fixed onto the surfaces of a negatively charged support material, the incubation being at a pH ranging from about 4.0 to about 6.5 and being under conditions sufficient to result in the formation of immunochemical complexes on the composite, some of which complexes include labeled cortisol; (b) separating the composite from the incubation medium; (c) determining the amount of label on the separated composite or in the remaining incubation medium; and (d) relating the determination of step (c) to a standard to determine the cortisol concentration in the sample.
Claim 3
Dependent Claim
16 words
The assay of claim 1 wherein the antibodies are fixed onto the surfaces of siliceous particles.
Claim 4
Dependent Claim
13 words
The assay of claim 1 wherein the siliceous particles are silanized siliceous particles.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the deblocking agent is 8-anilino-1-naphthalene sulfonic acid, or a salt thereof.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein the incubation step is for a period of less than about one hour.
Claim 7
Dependent Claim
13 words
The method of claim 1 wherein all steps are performed at room temperature.
Claim 8
Dependent Claim
17 words
The method of claim 1 wherein the labeled cortisol comprises a I 125 tyramine derivative of cortisol.
Claim 9
Dependent Claim
14 words
The method of claim 1 wherein the serum sample size is about 25 microliters.
References Cited (Backward Citations)
US4034073A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-12-04 | 1982-06-05 | 1982-12-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-12-04 | 1986-06-05 | 1986-12-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-12-04 | 1990-06-05 | 1990-12-05 | $7700.00 | Window Closed / Lapsed |