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US4116771A
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Immobilized saccharifying enzyme product and process for preparation thereof
Assignee: Novo Nordisk AS
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Filing: 1977-06-28
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Granted: 1978-09-26
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Expires: 1997-06-28
Abstract
Abstract An immobilized saccharifying enzyme product formed by coating granular casein with a liquid-permeable proteinaceous layer comprising saccharifying enzyme and egg albumen cross-linked by reaction with glutaraldehyde.
Inventors
- 👤 Shmuel Amotz
- 👤 Tage Kjaer Nielsen
- 👤 Poul Borge Rosenius Poulsen
- 👤 Barrie Edmund Norman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:38
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12P; C12P19/00; C12P19/14; C12P19/20; C13; C13K; C13K1/00; C13K1/06
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
50 words
An immobilized saccharifying enzyme product in particulate form, the saccharifying enzyme being selected from the group consisting of amyloglucosidase and maltogenic alpha-amylase, which product comprises carrier particles of granular casein having a coating of a liquid-permeable proteinaceous layer comprising the saccharifying enzyme and egg albumen cross-linked by reaction with glutaraldehyde.
Claim 2
Dependent Claim
50 words
An immobilized saccharifying enzyme product in particulate form, the saccharifying enzyme being selected from the group consisting of amyloglucosidase and maltogenic alpha-amylase, which product comprises carrier particles of granular casein having a coating of a liquid-permeable proteinaceous layer comprising the saccharifying enzyme and egg albumen cross-linked by reaction with glutaraldehyde.
Claim 3
Dependent Claim
24 words
The product of claim 1, in which the weight ratio of the amount saccharifying enzyme-casein is in the range of from 1:200 to 1:2.
Claim 4
Dependent Claim
25 words
The product of claim 1, in which the weight ratio of the amount of saccharifying enzyme:casein is in the range of from 1:25 to 1:5.
Claim 5
Dependent Claim
25 words
The product of claim 1, in which the weight ratio of the amount of saccharifying enzyme:albumen is in the range of from 1:5 to 1:0.05.
Claim 6
Dependent Claim
25 words
The product of claim 1, in which the weight ratio of the amount of saccharifying enzyme:albumen is in the range of from 1:2 to 1:0.2.
Claim 7
Dependent Claim
24 words
The product of claim 1, in which the particle diameter of the granular casein is in the range of from 100 to 500 microns.
Claim 8
Dependent Claim
13 words
The product of claim 1, in which the granular casein is acid-precipitated casein.
Claim 9
Dependent Claim
75 words
A process for the preparation of an immobilized saccharifying enzyme product in particulate form, the saccharifying enzyme being selected from the group consisting of amyloglucosidase and maltogenic alpha-amylase, which process comprises coating carrier particles of precipitated casein with a reaction mixture of the saccharifying enzyme, egg albumen and glutaraldehyde, whereby the granular casein becomes coated by a liquid-permeable proteinaceous layer, in which the saccharifying enzyme and egg albumen are cross-linked by reaction with the glutaraldehyde.
Claim 10
Dependent Claim
75 words
A process for the preparation of an immobilized saccharifying enzyme product in particulate form, the saccharifying enzyme being selected from the group consisting of amyloglucosidase and maltogenic alpha-amylase, which process comprises coating carrier particles of precipitated casein with a reaction mixture of the saccharifying enzyme, egg albumen and glutaraldehyde, whereby the granular casein becomes coated by a liquid-permeable proteinaceous layer, in which the saccharifying enzyme and egg albumen are cross-linked by reaction with the glutaraldehyde.
Claim 11
Dependent Claim
25 words
The process of claim 8, in which the weight ratio of the amount of saccharifying enzyme:casein is in the range of from 1:200 to 1:2.
Claim 12
Dependent Claim
25 words
The process of claim 8, in which the weight ratio of the amount of saccharifying enzyme:casein is in the range of from 1:25 to 1:5.
Claim 13
Dependent Claim
25 words
The process of claim 8, in which the weight ratio of the amount of saccharifying enzyme:albumen is in the range of from 1:5 to 1:0.05.
Claim 14
Dependent Claim
25 words
The process of claim 8, in which the weight ratio of the amount of saccharifying enzyme:albumen is in the range of from 1:2 to 1:0.2.
Claim 15
Dependent Claim
24 words
The process of claim 8, in which the particle diameter of the granular casein is in the range of from 100 to 500 microns.
Claim 16
Dependent Claim
13 words
The process of claim 8, in which the granular casein is acid-precipitated casein.
Claim 17
Dependent Claim
31 words
The process of claim 8, in which the weight ratio of glutaraldehyde to the enzyme plus albumen incorporated into the proteinaceous layer is in the range of from 0.1 to 1.
Claim 18
Dependent Claim
20 words
The process of claim 8, in which the immobilized saccharifying enzyme product is treated with an aqueous solution of glutaraldehyde.
Claim 19
Dependent Claim
24 words
The process of claim 8, in which the immobilized saccharifying enzyme product is treated with an aqueous solution of a salt of sulphurous acid.
Claim 20
Dependent Claim
25 words
The process of claim 16, in which the immobilized saccharifying enzyme product is thereafter treated with an aqueous solution of a salt of sulphurous acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-25 | 1982-03-27 | 1982-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-25 | 1986-03-27 | 1986-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-25 | 1990-03-27 | 1990-09-26 | $7700.00 | Window Closed / Lapsed |