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US4107014A
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Isoelectric point markers for gel isoelectric separation
Assignee: Oriental Yeast Co Ltd
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Filing: 1976-12-28
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Granted: 1978-08-15
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Expires: 1996-12-28
Abstract
Abstract Isoelectric point markers for a gel isoelectric separation are prepared to comprise at least two colored proteins of which the isoelectric point pHs are known and wherein the difference between the highest value and the lowest value among the said pHs is not less than 0.5. The markers are applied to analyze proteins in a gel isoelectric separation method. The method employing the said markers makes it easy to analyze proteins and shows an excellent performance.
Inventors
- 👤 Yasuo Suzuki
- 👤 Isamu Takagahara
- 👤 Tsuyoshi Fujita
- 👤 Katsumi Fujii
- 👤 Takekazu Horio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 23:27
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44795; G01N27/44704; G01N27/44717; G01N27/44721; G01N27/44726; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
43 words
Isoelectric point markers for a gel isoelectric separation, which comprise at least two colored proteins of which the isoelectric point pHs are known, and wherein the difference between the highest value and the lowest value among said pHs is not less than 0.5.
Claim 2
Dependent Claim
43 words
Isoelectric point markers for a gel isoelectric separation, which comprise at least two colored proteins of which the isoelectric point pHs are known, and wherein the difference between the highest value and the lowest value among said pHs is not less than 0.5.
Claim 3
Dependent Claim
27 words
Isoelectric point markers according to claim 1, wherein the colored proteins are cytachrome-cs, myoglobins, hemoglobins, flavoproteins, copper proteins, and the like, obtained from animals, plants and microorganisms.
Claim 4
Dependent Claim
21 words
Isoelectric point markers according to claim 1, wherein the isoelectric point pHs of the colored proteins are from 3.9 to 10.6.
Claim 5
Dependent Claim
65 words
A gel isoelectric separation method for the analysis of proteins by using isoelectric point markers which comprise at least two colored proteins of which the isoelectric point pHs are wherein the difference between the highest value and the lowest value among the said pHs is not less than 0.5, and wherein the migration distances of the said markers are used as indicators of pH value.
Claim 6
Dependent Claim
65 words
A gel isoelectric separation method for the analysis of proteins by using isoelectric point markers which comprise at least two colored proteins of which the isoelectric point pHs are wherein the difference between the highest value and the lowest value among the said pHs is not less than 0.5, and wherein the migration distances of the said markers are used as indicators of pH value.
Claim 7
Dependent Claim
31 words
A gel isoelectric separation method according to claim 4, wherein the colored proteins are cytochrome-cs, myoglobins, hemoglobins, flavoproteins, copper proteins and the like, various obtained from various animals, plants and microorganisms.
Claim 8
Dependent Claim
23 words
A gel isoelectric separation method according to Claim 4 wherein the isoelectric point pHs of the colored proteins are from 3.9 to 10.6.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-08-14 | 1982-02-13 | 1982-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-08-14 | 1986-02-13 | 1986-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-08-14 | 1990-02-13 | 1990-08-15 | $7700.00 | Window Closed / Lapsed |