Patent US4100029A successfully refreshed from Google Patents.
US4100029A
Expired / Lapsed
US
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Method for improving the activity of oxireductase enzymes embedded in filamentary structures
Assignee: Anic SpA
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Filing: 1976-07-09
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Granted: 1978-07-11
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Expires: 1996-07-09
Abstract
Abstract A method is disclosed for improving the activity of oxireductase enzymes, the improvement consisting in that an enzyme and a previously functionalized coenzyme are embedded in a filamentary structure, the coenzyme having previously been attached to a water-soluble, high-molecular-weight polymer.
Inventors
- 👤 Giulio Prosperi
- 👤 Walter Marconi
- 👤 Silvia Giovenco
- 👤 Franco Morisi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:01
CPC Classifications
D; D01; D01F; D01F1/00; D01F1/02; C; C07; C07J; C07J1/00; C07J1/0003; C07J1/0011; C07J5/00; C07J5/0046; C07J5/0053; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12P; C12P1/00; C12P13/00; C12P13/04; C12P13/06; C12P33/00; C12P33/06; C12Q; C12Q1/00; C12Q1/26
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
96 words
A method for improving the activity of oxidoreductase enzymes that are formed into a filamentary structure which comprises a fibrous or filamentous structural base of a water-insoluble artifical synthetic polymeric material and an enzyme or enzymes therein englobed and subdivided and partially enclosed in separated alveoli, said method comprising the steps of forming solutions of a structural base of a water-insoluble artifical synthetic polymeric material an oxidoreductase enzyme and a coenzyme of nicotinamide-adenine-dinucleotide and a water soluble, high molecular weight polymer, forming an emulsion of said solutions and thereafter spinning said emulsion into said filamentary structure.
Claim 2
Independent Claim
96 words
A method for improving the activity of oxidoreductase enzymes that are formed into a filamentary structure which comprises a fibrous or filamentous structural base of a water-insoluble artifical synthetic polymeric material and an enzyme or enzymes therein englobed and subdivided and partially enclosed in separated alveoli, said method comprising the steps of forming solutions of a structural base of a water-insoluble artifical synthetic polymeric material an oxidoreductase enzyme and a coenzyme of nicotinamide-adenine-dinucleotide and a water soluble, high molecular weight polymer, forming an emulsion of said solutions and thereafter spinning said emulsion into said filamentary structure.
Claim 3
Dependent Claim
29 words
The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate and polyethyleneimine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.
Claim 4
Dependent Claim
28 words
The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, formylpolyethyleneimine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.
Claim 5
Dependent Claim
28 words
The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, polylysine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.
Claim 6
Dependent Claim
28 words
The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate polyethyleneimine-nicotinamide-adenine-dinucleotide, beta-hydroxysteroid dehydrogenase, alcohol dehydrogenase and aldehyde dehydrogenase.
Claim 7
Dependent Claim
25 words
The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, polyethyleneimine-nicotinamide-adenine-dinucleotide, beta-hydroxysteroid dehydrogenase and diaphorase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-10 | 1982-01-09 | 1982-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-10 | 1986-01-09 | 1986-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-10 | 1990-01-09 | 1990-07-11 | $7700.00 | Window Closed / Lapsed |