US3957748A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Nicotinamide-adenine-dinucleotide chemically coupled to water-insoluble carriers
Assignee: Corning Glass Works
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Filing: 1971-07-30
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Granted: 1976-05-18
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Expires: 1991-07-30
Abstract
Abstract Nicotinamide-adenine-dinucleotide (NAD) can be coupled chemically to an essentially water-insoluble carrier material selected from the group consisting of glass particles having a diazotizable silane coupling agent attached thereto, p-aminobenzylcellulose, polyaminopolystyrene, and the half amide of benzidine-carboxymethylcellulose by diazotizing the carrier materials and reacting the diazotized materials with a solution of NAD. Methods of chemically coupling the NAD to such carriers are disclosed and the redox activity of NAD coupled to porous glass beads through an intermediate silane coupling agent is demonstrated.
Inventors
- 👤 Howard H. Weetall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:38
CPC Classifications
C; C12; C12N; C12N11/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
81 words
A method of preparing a composite consisting of nicotinamide-adenine-dinucleotide coupled chemically to a carrier material in such a manner that the nicotinamide-adenine-dinucleotide does not lose its redox properties which comprises the steps of diazotizing a carrier material selected from the group consisting of glass particles having a diazotizable silane coupling agent attached thereto, p-aminobenzylcellulose, polyaminopolystyrene, and the half amide of benzidine-carboxymethylcellulose to form a diazo salt intermediate, and immediately thereafter reacting the diazotized carrier material with an aqueous solution of nicotinamide-adenine-dinucleotide.
Claim 2
Independent Claim
81 words
A method of preparing a composite consisting of nicotinamide-adenine-dinucleotide coupled chemically to a carrier material in such a manner that the nicotinamide-adenine-dinucleotide does not lose its redox properties which comprises the steps of diazotizing a carrier material selected from the group consisting of glass particles having a diazotizable silane coupling agent attached thereto, p-aminobenzylcellulose, polyaminopolystyrene, and the half amide of benzidine-carboxymethylcellulose to form a diazo salt intermediate, and immediately thereafter reacting the diazotized carrier material with an aqueous solution of nicotinamide-adenine-dinucleotide.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the carrier material consists of porous glass particles and the silane coupling agent is alpha-aminopropyltriethoxysilane.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the carrier material consists of glass beads and the silane coupling agent is alpha-aminopropyltriethoxy-silane.
Claim 5
Dependent Claim
15 words
The method of claim 1 wherein the diazotization temperature is between about 4° and 20°C.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein the solution of nicotinamide-adenine-dinucleotide has a concentration of 10 - 2 M buffered to a pH of 8.5.
Claim 7
Dependent Claim
19 words
A composite consisting of nicotinamide-adenine-dinucleotide coupled chemically to a carrier material in accordance with the method of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-05-18 | 1979-11-17 | 1980-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-05-18 | 1983-11-17 | 1984-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-05-18 | 1987-11-17 | 1988-05-18 | $7700.00 | Window Closed / Lapsed |