Patent US4094963A successfully refreshed from Google Patents.
US4094963A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Means for testing for pregnancy
Assignee: Cornell Research Foundation Inc
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Filing: 1976-05-20
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Granted: 1978-06-13
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Expires: 1996-05-20
Abstract
Abstract Disclosed is a method for the determination of the hormone human chorionic gonadotropin (HCG), luteinizing hormone (LH), prolactin (PRL) and HCG-like material in an aqueous sample comprising contacting a highly specific receptor for these hormones with an aqueous sample to be tested, providing a means of indicating whether binding has taken place between the receptor and hormone possibly contained the sample and observing the indicating means to determine the presence of the hormone in the sample. PRL may be determined separately or simultaneously with the HCG and LH. The receptor is a plasma membrane extract from the corpus luteum of an animal which possesses the receptor for HCG, LH, PRL and HCG-like material. Radioassay indicating means are preferred, according to which competitive protein binding between radioisotopically labeled hormone and hormone present in the sample is radiologically determined. The method has particular application to the determination to pregnancy in the human female.
Inventors
- 👤 Brij B. Saxena
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 13:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
144 words
Means for the determination of human chorionic gonadotropin (HCG), luteinizing hormone (LH) or HCG-like material in an aqueous sample, comprising (a) a container having therein a first reagent ; and (b) a separate second container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for human chorionic gonadotropin capable of selectively binding biologically active human chorionic gonadotropin; and (d) said second reagent comprising labeled human chorionic gonadotropin capable of emitting radiation, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagent to bind part of the labeled and unlabeled hormone to said receptor the emitted radiation therefrom being a function of the concentration of the hormone in the aqueous sample.
Claim 2
Dependent Claim
144 words
Means for the determination of human chorionic gonadotropin (HCG), luteinizing hormone (LH) or HCG-like material in an aqueous sample, comprising (a) a container having therein a first reagent ; and (b) a separate second container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for human chorionic gonadotropin capable of selectively binding biologically active human chorionic gonadotropin; and (d) said second reagent comprising labeled human chorionic gonadotropin capable of emitting radiation, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagent to bind part of the labeled and unlabeled hormone to said receptor the emitted radiation therefrom being a function of the concentration of the hormone in the aqueous sample.
Claim 3
Dependent Claim
12 words
Means as defined by claim 1, in the form of a kit.
Claim 4
Dependent Claim
22 words
The means as defined by claim 2, further comprising a separate third container having therein a control sample of human chorionic gonadotropin.
Claim 5
Dependent Claim
129 words
Means for the determination of prolactin (PRL) in an aqueous sample, comprising (a) a container having therein a first reagent; and (b) a second separate container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for prolactin capable of selectively binding biologically active prolactin; and (d) said second reagent comprising labeled prolactin capable of emitting radiation, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagent to bind part of the labeled and unlabeled hormone to said receptor the emitted radiation therefrom being a function of the concentration of the hormone in the aqueous sample.
Claim 6
Dependent Claim
129 words
Means for the determination of prolactin (PRL) in an aqueous sample, comprising (a) a container having therein a first reagent; and (b) a second separate container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for prolactin capable of selectively binding biologically active prolactin; and (d) said second reagent comprising labeled prolactin capable of emitting radiation, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagent to bind part of the labeled and unlabeled hormone to said receptor the emitted radiation therefrom being a function of the concentration of the hormone in the aqueous sample.
Claim 7
Dependent Claim
172 words
Means for the determination of a plurality of hormones in an aqueous sample, comprising (a) a container having therein a first reagent; and (b) a number of second containers equal to the number of hormones to be determined, each second container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from a body organ of a species having the specific receptor in said organ for selectively binding each of said hormones in biologically active form; and (d) said second reagents comprising each of said hormones to be determined in a labeled form capable of emitting radiation, said emitted radiation being different for each labeled hormone, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagents to bind part of the labeled and unlabeled hormones to said receptor the emitted radiation from each labeled hormone being a function of the concentration of the respective hormone in the aqueous sample.
Claim 8
Dependent Claim
172 words
Means for the determination of a plurality of hormones in an aqueous sample, comprising (a) a container having therein a first reagent; and (b) a number of second containers equal to the number of hormones to be determined, each second container having therein a second reagent; (c) said first reagent comprising in substantially pure form the specific fraction of plasma membrane extract from a body organ of a species having the specific receptor in said organ for selectively binding each of said hormones in biologically active form; and (d) said second reagents comprising each of said hormones to be determined in a labeled form capable of emitting radiation, said emitted radiation being different for each labeled hormone, said first reagent being intended to be contacted with the sample containing the hormone to be measured and with the second reagents to bind part of the labeled and unlabeled hormones to said receptor the emitted radiation from each labeled hormone being a function of the concentration of the respective hormone in the aqueous sample.
References Cited (Backward Citations)
US3899298A
Cited
US3988429A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-12 | 1981-12-12 | 1982-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-12 | 1985-12-12 | 1986-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-12 | 1989-12-12 | 1990-06-13 | $7700.00 | Window Closed / Lapsed |