Patent US3402223A successfully refreshed from Google Patents.
Patent US4093606A successfully refreshed from Google Patents.
US4093606A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing intravenously injectable gamma globulin and a gamma globulin suitable for carrying out the method
Assignee: Individual
•
Filing: 1976-05-21
•
Granted: 1978-06-06
•
Expires: 1996-05-21
Abstract
Abstract A process is provided for the preparation of a gamma globulin suitable for intravenous administration from the readily available Fraction II or II + III plasma protein paste. The Fraction II or II + III paste is extracted with water at a pH of 4.8 - 6.5 and impurities are fractionally precipitated by addition of polyethylene glycol to 4% of wt./vol. and then 5% wt./vol. The desired gamma globulin is then precipitated at a pH 8.0 by addition of polyethylene glycol to 12% wt./vol.
Inventors
- 👤 Myer Louis Coval
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:51
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
137 words
A process for preparing a gamma globulin substantially devoid of anticomplementary activity and suitable for intravenous administration, from a material selected from the Fraction II + III plasma protein paste having a protein content of about 25-30% and Fraction II paste which comprises the steps: a. suspending said paste in water to form a solution of low ionic strength having a conductance of about 300 × 10 -6 cm -6 ohm -1 at a pH of about 4.8 to 6.5 to produce a precipitate and filtrate, b. fractionally precipitating impurities from said filtrate by adding polyethylene glycol to 4% weight/volume and then 5% weight/volume, c. precipitating the gamma globulin by adding polyethylene glycol to 12% weight/volume at a pH of about 8.0. said process being carried out at a temperature of about 0° to 20 ° C.
Claim 2
Dependent Claim
137 words
A process for preparing a gamma globulin substantially devoid of anticomplementary activity and suitable for intravenous administration, from a material selected from the Fraction II + III plasma protein paste having a protein content of about 25-30% and Fraction II paste which comprises the steps: a. suspending said paste in water to form a solution of low ionic strength having a conductance of about 300 × 10 -6 cm -6 ohm -1 at a pH of about 4.8 to 6.5 to produce a precipitate and filtrate, b. fractionally precipitating impurities from said filtrate by adding polyethylene glycol to 4% weight/volume and then 5% weight/volume, c. precipitating the gamma globulin by adding polyethylene glycol to 12% weight/volume at a pH of about 8.0. said process being carried out at a temperature of about 0° to 20 ° C.
Claim 3
Dependent Claim
20 words
A process according to claim 1 wherein the pH at which the paste is extracted is about 5.7 to 5.9.
Claim 4
Dependent Claim
27 words
A process according to claim 2 wherein 1 kg. of Fraction II + III paste is extracted at pH 5.8 with about 30 liters of pyrogen-free water.
Claim 5
Dependent Claim
19 words
A process according to claim 1 wherein the polyethylene glycol has a molecular weight of about 4,000 to 6,000.
Claim 6
Dependent Claim
17 words
A process according to claim 4 wherein the polyethylene glycol has a molecular weight of about 4,000.
Claim 7
Dependent Claim
21 words
A process according to claim 5 wherein the process is carried out at a temperature of about 0° to 5° C.
References Cited (Backward Citations)
US2437060A
Cited
US2793203A
Cited
US2867567A
Cited
GB1198277A
Cited
US3916026A
Cited
US3869436A
Cited
US3763135A
Cited
US3880989A
Cited
US4000121A
Cited
US3808189A
Cited
US4018885A
Cited
US3984539A
Cited
US3943245A
Cited
US4025500A
Cited
US4021540A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-05 | 1981-12-05 | 1982-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-05 | 1985-12-05 | 1986-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-05 | 1989-12-05 | 1990-06-06 | $7700.00 | Window Closed / Lapsed |