Bovine immunoglobulin isolation process

Claim 2

A process for preparing a bovine immunoglobulin fraction active against coliform bacteria infection in young calves, which comprises the steps of precipitating out by salt fractionation crude immunoglobulins from bovine plasma or clear bovine serum employing a salt concentration of up to about 0.7 saturation, and removing the crude immunoglobulins so formed; forming an aqueous solution of the crude immunoglobulins in an aqueous solution of up to about 20% (w/v) salt concentration, heating the solution to a temperature of from about 50° C to about 60° C and cooling the solution and then removing coagulated proteins; precipitating out the immunoglobulins by salt fractionation employing a salt concentration of about 0.2 to about 0.6 saturation; separating and redissolving the precipitate in aqueous solution, and purifying the solution by subjection to a molecular sieve procedure.

Claim 3

The process of claim 1 wherein the solution which is heated is a solution of crude immunoglobulins in aqueous sodium chloride solution, of up to 20% (w/v) sodium chloride concentration.

Claim 4

The process of claim 2 wherein the sodium chloride concentration is about 10% w/v.

Claim 5

The process of claim 1 wherein the purification is effected by dialysis against dilute saline.

Claim 6

The process of claim 1 which is carried out on clear bovine serum.

Claim 7

The process of claim 1 which is carried out on bovine plasma.

Claim 8

The process off claim 3 wherein the heating takes place at about 56° C for about 15 minutes.

Claim 9

The process of claim 1 including the additional step of adding a preservative to the purified solution.

Claim 10

The process of claim 8 wherein the preservative is selected from the group consisting of thimerosal and phenol.

Claim 11

The pro cess of claim 1 which includes the step of freeze drying the immunoglobulins after purification.

Claim 12

A process as claimed in claim 1 wherein the heating takes place at about 56° C for a short length of time sufficient to inactivate viruses which are present.

Claim 13

The process as claimed in claim 1 wherein, after purifying the solution by subjection to a molecular sieve procedure, the solution is sterile filtered and the filtrate containing bovine immunoglobulin is recovered.

Claim 14

A process for preparing a bovine immunoglobulin fraction active against coliform bacteria infection in young calves, which comprises the steps of a. subjecting bovine plasma or clear bovine serum to salt fractionation with ammonium sulfate of up to about 0.7 saturation, and recovering the crude immunoglobulins that are precipitated; b. forming aqueous solution of said crude immunoglobulins in an aqueous sodium chloride solution of up to about 20% (w/v) sodium chloride concentration; c. heating said aqueous solution to a temperature of from about 50° C to 60° C; cooling the solution and then removing coagulated proteins; d. thereafter subjecting said solution to salt fractionation with ammonium sulfate of about 0.2 to about 0.6 saturation to obtain a precipitate of immunoglobulins; e. separating and redissolving the precipitate in aqueous solution; and f. purifying the precipitate by subjection to a molecular sieve procedure.

Claim 15

A process for preparing a bovine immunoglobulin fraction active against coliform bacteria infection in young calves, which comprises the steps of a. subjecting bovine plasma or clear bovine serum to salt fractionation with ammonium sulfate of up to about 0.7 saturation, and recovering the crude immunoglobulins that are precipitated; b. forming aqueous solution of said crude immunoglobulins in an aqueous sodium chloride solution of up to about 20% (w/v) sodium chloride concentration; c. heating said aqueous solution to a temperature of from about 50° C to 60° C; cooling the solution and then removing coagulated proteins; d. thereafter subjecting said solution to salt fractionation with ammonium sulfate of about 0.2 to about 0.6 saturation to obtain a precipitate of immunoglobulins; e. separating and redissolving the precipitate in aqueous solution; and f. purifying the precipitate by subjection to a molecular sieve procedure.

Claim 16

A process as claimed in claim 13 wherein said heating step (c) takes place at about 56° C for a short length of time sufficient to inactivate viruses which are present.

Claim 17

A process as claimed in claim 14 wherein said heating step takes place for about 15 minutes.

Claim 18

The process as claimed in claim 13 wherein, after purifying the solution by subjection to a molecular sieve procedure, the solution is sterile filtered and the filtrate containing bovine immunoglobulin is recovered.