Process for separating and recovering globulins from blood plasmas

Claim 1

THE METHOD OF RECOVERING ANTIBODY-RICH GAMMA GLOBULINS FROM BLOOD PLASMAS, WHICH COMPRISES ADDING ETHANOL TO A CITRATED PLASMA UNTIL SUBSTANTIALLY TEN PER CENT ETHANOL CONCENTRATION IS REACHED, REMOVING THE RESULTING PRECIPITATE, ADDING ETHANOL TO THE SUPERNATANT FLUID TO AN ETHANOL CONCENTRATION OF SUBSTANTIALLY 25%, WHILE MAINTAINING THE TEMPERATURE OF THE FLUID AT APPROXIMATELY -5*C. DURING ADDITION OF THE ETHANOL, SEPARATING THE RESULTING PRECIPITATE, EXTRACTING THE PRECIPITATE THE REMOVE SERUM GAMMA GLOBULIN THEREFROM, SUSPENDING THE RESULTING RESIDUE IN PYROGEN-FREE WATER AT SUBSTANTIALLY 0*C., ADJUSTING THE PH VALUE OF THE RESULTING SUSPENSION TO SUBSTANTIALLY PH 4.8 AGITATING THE RESULTING MATERIAL AT SUBSTANTIALLY 0*C., MAINTAINING THE MATERIAL AT 0*C. DURING PROLONGED AGITATION THEREOF, ADJUSTING THE PH VALUE OF THE RESULTING MATERIAL TO PH 5.1, ADDING ETHANOL THERETO UNTIL AN ETHANOL CONCENTRATION OF 10% IS REACHED, STIRRING THE RESULTING SUSPENSION AT SUBSTANTIALLY -3*C. SUBSEQUENTLY TO THIS ADDITION OF ETHANOL, SEPARATING THE RESULTING PRECIPITATE WHILE REFRIGERATING THE MIXTURE, ADJUSTING THE PH VALUE OF THE RESULTING LIQUID TO SUBSTANTIALLY PH 7.65, ADDING ETHANOL UNTIL 20% CONCENTRATION IS REACHED, WHILE MAINTAINING THE MATERIAL AT A MAXIMUM TEMPERATURE OF -5*C., CENTRIFUGING OFF THE RESULTING PRECIPITATE AT THE SAID TEMPERATURE, SUSPENDING THE RESULTING SEPARATED PRECIPITATE IN COLD DISTILLED WATER, FREEZING THE RESULTING MIXTURE, AND DRYING THE SAID MIXTURE IN VACUO.

Claim 2

THE METHOD OF RECOVERING ANTIBODY-RICH GAMMA GLOBULINS FROM BLOOD PLASMAS, WHICH COMPRISES ADDING ETHANOL TO A CITRATED PLASMA UNTIL SUBSTANTIALLY TEN PER CENT ETHANOL CONCENTRATION IS REACHED, REMOVING THE RESULTING PRECIPITATE, ADDING ETHANOL TO THE SUPERNATANT FLUID TO AN ETHANOL CONCENTRATION OF SUBSTANTIALLY 25%, WHILE MAINTAINING THE TEMPERATURE OF THE FLUID AT APPROXIMATELY -5*C. DURING ADDITION OF THE ETHANOL, SEPARATING THE RESULTING PRECIPITATE, EXTRACTING THE PRECIPITATE THE REMOVE SERUM GAMMA GLOBULIN THEREFROM, SUSPENDING THE RESULTING RESIDUE IN PYROGEN-FREE WATER AT SUBSTANTIALLY 0*C., ADJUSTING THE PH VALUE OF THE RESULTING SUSPENSION TO SUBSTANTIALLY PH 4.8 AGITATING THE RESULTING MATERIAL AT SUBSTANTIALLY 0*C., MAINTAINING THE MATERIAL AT 0*C. DURING PROLONGED AGITATION THEREOF, ADJUSTING THE PH VALUE OF THE RESULTING MATERIAL TO PH 5.1, ADDING ETHANOL THERETO UNTIL AN ETHANOL CONCENTRATION OF 10% IS REACHED, STIRRING THE RESULTING SUSPENSION AT SUBSTANTIALLY -3*C. SUBSEQUENTLY TO THIS ADDITION OF ETHANOL, SEPARATING THE RESULTING PRECIPITATE WHILE REFRIGERATING THE MIXTURE, ADJUSTING THE PH VALUE OF THE RESULTING LIQUID TO SUBSTANTIALLY PH 7.65, ADDING ETHANOL UNTIL 20% CONCENTRATION IS REACHED, WHILE MAINTAINING THE MATERIAL AT A MAXIMUM TEMPERATURE OF -5*C., CENTRIFUGING OFF THE RESULTING PRECIPITATE AT THE SAID TEMPERATURE, SUSPENDING THE RESULTING SEPARATED PRECIPITATE IN COLD DISTILLED WATER, FREEZING THE RESULTING MIXTURE, AND DRYING THE SAID MIXTURE IN VACUO.