Patent US4091201A successfully refreshed from Google Patents.
US4091201A
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Process for recovering antibiotics U-50,147 and U-51,738
Assignee: Pharmacia and Upjohn Co
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Filing: 1977-03-09
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Granted: 1978-05-23
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Expires: 1997-03-09
Abstract
Abstract Microbiological process for preparing the known antibiotics, antibiotic A-396-1 and L-dihydrophenylalanine, by use of the new microorganism Streptomyces lemensis, NRRL 8170 using controlled fermentation conditions. These antibiotics are active against Gram-positive and Gram-negative bacteria. Accordingly, they can be used in various environments to eradicate or control such bacteria.
Inventors
- 👤 Alexander D. Argoudelis
- 👤 LeRoy E. Johnson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:28
CPC Classifications
C; C07; C07G; C07G11/00; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
127 words
A process for recovering antibiotic U-50,147 from a fermentation mixture f antibiotics U-50,147 and U-51,738 which comprises: (a) filtering a fermentation beer containing antibiotic U-50,147 and antibiotic U-51,738 to obtain a clear beer; (2) passing said clear beer over a non-ionic resin and collecting a spent filtrate; (3) washing said resin with water and collecting an aqueous wash; (4) combining said spent and aqueous wash and chromatographing them over a cationic exchange resin and eluting said resin with a basic solution to obtain fractions containing antibiotic U-50,147; (5) passing said fractions containing antibiotic U-50,147 over a strongly basic anion exchange resin and eluting said resin with water and collecting fractions; and (6) concentrating said fractions containing antibiotic U-50,147 to give an essentially pure preparation of antibiotic U-50,147.
Claim 2
Dependent Claim
127 words
A process for recovering antibiotic U-50,147 from a fermentation mixture f antibiotics U-50,147 and U-51,738 which comprises: (a) filtering a fermentation beer containing antibiotic U-50,147 and antibiotic U-51,738 to obtain a clear beer; (2) passing said clear beer over a non-ionic resin and collecting a spent filtrate; (3) washing said resin with water and collecting an aqueous wash; (4) combining said spent and aqueous wash and chromatographing them over a cationic exchange resin and eluting said resin with a basic solution to obtain fractions containing antibiotic U-50,147; (5) passing said fractions containing antibiotic U-50,147 over a strongly basic anion exchange resin and eluting said resin with water and collecting fractions; and (6) concentrating said fractions containing antibiotic U-50,147 to give an essentially pure preparation of antibiotic U-50,147.
Claim 3
Dependent Claim
13 words
A process, according to claim 1, wherein said non-ionic resin is Amberlite XAD-4.
Claim 4
Dependent Claim
17 words
A process, according to claim 1, wherein said cation exchange resin is Amberlite IRC-50 (H + ).
Claim 5
Dependent Claim
19 words
A process, according to claim 1, wherein said cation exchange resin is eluted wit 0.25 N aqueous ammonium hydroxide.
Claim 6
Dependent Claim
17 words
A process, according to claim 1, wherein said anion exchange resin is Dowex-1 l (OH - ).
Claim 7
Dependent Claim
71 words
A process for recovering antibiotic U-51,738 from a fermentation mixture of antibiotics U-51,738 and U-50,147 which comprises: (1) filtering a fermentation beer containing antibiotic U-51,738 and antibiotic U-50,147 to obtain a clear beer; (2) passing said clear beer over a non-ionic resin and eluting said resin with a lower-alkanol-water solution to obtain fractions containing antibiotic U-51,738; and (3) concentrating said fractions to give an essentially pure crystalline preparation of antibiotic U-51,738.
Claim 8
Dependent Claim
71 words
A process for recovering antibiotic U-51,738 from a fermentation mixture of antibiotics U-51,738 and U-50,147 which comprises: (1) filtering a fermentation beer containing antibiotic U-51,738 and antibiotic U-50,147 to obtain a clear beer; (2) passing said clear beer over a non-ionic resin and eluting said resin with a lower-alkanol-water solution to obtain fractions containing antibiotic U-51,738; and (3) concentrating said fractions to give an essentially pure crystalline preparation of antibiotic U-51,738.
Claim 9
Dependent Claim
13 words
A process, according to claim 6, wherein said non-ionic resin is Amberlite XAD-4.
Claim 10
Dependent Claim
13 words
A process, according to claim 6, wherein said lower-alkanol-water solution is methanol-water (90:10).
References Cited (Backward Citations)
US4029548A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-22 | 1981-11-21 | 1982-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-22 | 1985-11-21 | 1986-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-22 | 1989-11-21 | 1990-05-23 | $7700.00 | Window Closed / Lapsed |