Patent US4069352A successfully refreshed from Google Patents.
US4069352A
Expired / Lapsed
US
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Immunoadsorbent polymeric material and method of making same
Assignee: Baxter Healthcare Corp
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Filing: 1976-07-02
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Granted: 1978-01-17
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Expires: 1996-07-02
Abstract
Abstract Gamma globulins from antisera having a titer so low in selected antibodies that the antibodies adsorbed on a surface of polymeric material are ineffective for use in immunoassay, are cross-linked, then adsorbed on the polymeric surface to provide an immunologically active coating. Binding proteins incapable of effective adsorption on polymeric surfaces are cross-linked with gamma globulin, then adsorbed on a surface of polymeric material capable of adsorbing gamma globulin to provide a specific binding protein coating. Kits containing test tubes having such coatings are used in radioimmunoassay, in enzyme immunoassay, and in fluoroimmunoassay.
Inventors
- 👤 George H. Parsons, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; Y; Y10; Y10S; Y10S435/00; Y10S435/975
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
61 words
A method of making a solid immunoadsorbent for use in immunoassay procedures which comprises cross-linking the gamma globulin content of low titer antiserum, and bringing the cross-linked gamma globulin at pH 8 to 10 into contact with a surface of a solid polymeric material capable of adsorbing gamma globulin to form a coating of cross-linked gamma globulin adsorbed on said surface.
Claim 2
Independent Claim
61 words
A method of making a solid immunoadsorbent for use in immunoassay procedures which comprises cross-linking the gamma globulin content of low titer antiserum, and bringing the cross-linked gamma globulin at pH 8 to 10 into contact with a surface of a solid polymeric material capable of adsorbing gamma globulin to form a coating of cross-linked gamma globulin adsorbed on said surface.
Claim 3
Dependent Claim
30 words
A method as claimed in claim 1 in which the cross-linking is carried out by adding to the gamma globulin a difunctional reagent capable of forming covalent bonds with proteins.
Claim 4
Dependent Claim
13 words
A method as claimed in claim 2 in which the reagent is glutaraldehyde.
Claim 5
Dependent Claim
14 words
A method as claimed in claim 2 in which the reagent is dimethyl suberimidate.
Claim 6
Dependent Claim
14 words
A method as claimed in claim 2 in which the reagent is a carbodiimide.
Claim 7
Dependent Claim
14 words
A method as claimed in claim 2 in which the reagent is sodium periodate.
Claim 8
Dependent Claim
19 words
A method as claimed in claim 1 in which the cross-linking is carried out by heating the gamma globulin.
Claim 9
Dependent Claim
14 words
A method as claimed in claim 1 in which the polymeric material is polypropylene.
Claim 10
Independent Claim
62 words
A method of making a solid immunoadsorbent for use in immunoassay procedures which comprises cross-linking a binding protein with gamma globulin to form a cross-linked material, and bringing the cross-linked material at pH 8 to 10 into contact with a surface of a solid polymeric material capable of adsorbing gamma globulin to form a coating of cross-linked material adsorbed on said surface.
Claim 11
Independent Claim
62 words
A method of making a solid immunoadsorbent for use in immunoassay procedures which comprises cross-linking a binding protein with gamma globulin to form a cross-linked material, and bringing the cross-linked material at pH 8 to 10 into contact with a surface of a solid polymeric material capable of adsorbing gamma globulin to form a coating of cross-linked material adsorbed on said surface.
Claim 12
Dependent Claim
33 words
A method as claimed in claim 9 in which the cross-linking is carried out by mixing said binding protein and gamma globulin with a difunctional reagent capable of forming covalent bonds with proteins.
Claim 13
Dependent Claim
13 words
A method as claimed in claim 10 in which the reagent is glutaraldehyde.
Claim 14
Dependent Claim
14 words
A method as claimed in claim 11 in which the reagent is dimethyl suberimidate.
Claim 15
Dependent Claim
25 words
A method as claimed in claim 9 in which the cross-linking is carried out by mixing said binding protein and gamma globulin with a carbodiimide.
Claim 16
Dependent Claim
25 words
A method as claimed in claim 13 in which the cross-linking is carried out by mixing said binding protein and gamma globulin with sodium periodate.
Claim 17
Dependent Claim
25 words
A method as claimed in claim 9 in which the cross-linking is carried out by heating a mixture of said binding protein and gamma globulin.
Claim 18
Dependent Claim
14 words
A method as claimed in claim 9 in which the polymeric material is polypropylene.
Claim 19
Dependent Claim
65 words
In a kit for determination of proteins, drugs, steroid hormones and other physiologically active materials in aqueous samples comprising a test tube having an inner surface of solid polymeric material capable of adsorbing gamma globulin and a supply of labeled tracer, the improvement comprising a coating on said surface selected from the group consisting of cross-linked gamma globulin and binding protein cross-linked to gamma globulin.
Claim 20
Dependent Claim
65 words
In a kit for determination of proteins, drugs, steroid hormones and other physiologically active materials in aqueous samples comprising a test tube having an inner surface of solid polymeric material capable of adsorbing gamma globulin and a supply of labeled tracer, the improvement comprising a coating on said surface selected from the group consisting of cross-linked gamma globulin and binding protein cross-linked to gamma globulin.
Claim 21
Dependent Claim
14 words
A kit as claimed in claim 17 in which the polymeric material is polypropylene.
Claim 22
Dependent Claim
15 words
A kit as claimed in claim 18 in which the tracer is radioactively labeled antigen.
Claim 23
Dependent Claim
15 words
A kit as claimed in claim 18 in which the tracer is enzyme labeled antigen.
Claim 24
Dependent Claim
15 words
A kit as claimed in claim 18 in which the tracer is fluorophore labeled antigen.
References Cited (Backward Citations)
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US3646346A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-16 | 1981-07-18 | 1982-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-16 | 1985-07-18 | 1986-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-16 | 1989-07-18 | 1990-01-17 | $7700.00 | Window Closed / Lapsed |