Patent US4067964A successfully refreshed from Google Patents.
US4067964A
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Antihemophilic agent and process for its manufacture
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1976-11-05
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Granted: 1978-01-10
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Expires: 1996-11-05
Abstract
Abstract The invention relates to an antihemophilic agent, which may contain factor VIII, but also factor IV, VII and X activities and to a process for its manufacture by extracting placentae with an aqueous hypotonic medium, increasing the density of the extract and obtaining the supernatant layer forming thereupon.
Inventors
- 👤 Horst Schwinn
- 👤 Norbert Heimburger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 19:58
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/647; A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/50; A61P; A61P7/00; A61P7/04; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
97 words
A method for making an antihemophilic agent, which method comprises comminuting placental tissue, washing the comminuted tissue with an aqueous hypotonic medium at a slightly acid to alkaline pH until the tissue is free of blood, extracting the washed tissue at a pH above 4.5 with an aqueous solution having an osmolarity below 330 nanomoles per liter, removing residual tissue from the extract, adding an inert water-soluble compound to the extract, whereby its density is increased, until separation and the flotation of a supernatant layer occur, and then isolating the supernatant layer, which contains said antihemophilic agent.
Claim 2
Independent Claim
97 words
A method for making an antihemophilic agent, which method comprises comminuting placental tissue, washing the comminuted tissue with an aqueous hypotonic medium at a slightly acid to alkaline pH until the tissue is free of blood, extracting the washed tissue at a pH above 4.5 with an aqueous solution having an osmolarity below 330 nanomoles per liter, removing residual tissue from the extract, adding an inert water-soluble compound to the extract, whereby its density is increased, until separation and the flotation of a supernatant layer occur, and then isolating the supernatant layer, which contains said antihemophilic agent.
Claim 3
Dependent Claim
16 words
A method as in claim 1 wherein said isolated supernatant layer is subjected to further purification.
Claim 4
Dependent Claim
17 words
A method as in claim 2 wherein said supernatant liquid is further purified by repeating the flotation.
Claim 5
Dependent Claim
82 words
A method as in claim 2 wherein said supernatant layer is further purified by diluting it with an aqueous medium and then extracting it, at low electrical conductivity and a slightly acid pH, with a water-immiscible solvent for lipids, in the presence of a coagulation-inert protective colloid and of a polar polyionic compound capable of forming a complex with polypeptides or proteins, whereupon the antihemophilic agent is enriched in the aqueous phase with respect to factor VIII--, IX--, VII--, and X-- activity.
Claim 6
Dependent Claim
40 words
A method as in claim 2 wherein said supernatant layer is further purified by combining it with aqueous sodium hydroxide solution, neutralizing the mixture to a pH between 6 and 8, and dialyzing it to remove low molecular weight constituents.
Claim 7
Dependent Claim
49 words
A method as in claim 2 wherein said supernatant layer containing factors VII, VIII, IX, and X, is further purified by dispersing it in an aqueous salt solution, chromatographing the mixture on a molecular sieve using an eluent containing a coagulation-inert protein, and collecting those fractions containing factor VIII.
Claim 8
Dependent Claim
49 words
A method as in claim 2 wherein said supernatant layer is further purified by dispersing it in an aqueous salt solution, adding a coagulation-inert protein to the mixture, centrifuging the mixture to separate a new supernatant layer, and isolating said new supernatant layer, which is enriched in factor VIII.
Claim 9
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 1.
Claim 10
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 2.
Claim 11
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 3.
Claim 12
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 4.
Claim 13
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 5.
Claim 14
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 6.
Claim 15
Dependent Claim
10 words
An antihemophilic agent prepared by the method of claim 7.
Claim 16
Dependent Claim
25 words
A pharmaceutical composition for the treatment of hemophilia which comprises an antihemophilic agent as in claim 9 in combination with an intravenously administrable pharmaceutical carrier.
Claim 17
Dependent Claim
34 words
The method of treating hemophilia by substitution therapy which comprises intravenously administering to a patient suffering from hemophilia an amount of an antihemophilic agent as in claim 9 sufficient to correct coagulation factor deficiencies.
References Cited (Backward Citations)
US3862002A
Cited
US3862314A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-09 | 1981-07-11 | 1982-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-09 | 1985-07-11 | 1986-01-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-09 | 1989-07-11 | 1990-01-10 | $7700.00 | Window Closed / Lapsed |