US3862002A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of physiologically active placental substances
Assignee: SANFAR LAB Inc
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Filing: 1967-07-03
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Granted: 1975-01-21
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Expires: 1987-07-03
Abstract
Abstract A system for the production of physiologically active placental substances in which viable placental tissue is placed in a circulating culture medium. The pH and temperature conditions are controlled and are continued for a sufficient period of time for the desired substance to be produced in the medium. The processed culture medium is then removed from the tissue, and the desired substances withdrawn, separated or otherwise recovered.
Inventors
- 👤 George R Sanders
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 00:47
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/50; C; C12; C12M; C12M21/00; C12M21/08; C12M47/00; C12M47/10; C12M47/12
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
64 words
The process of claim 1 wherein said fatty acids are separated from said ether phase by contacting said fatty acid extract with alcoholic KOH to saponify fatty acid esters therein, said saponified fatty acids are removed from the extract by contact with a water phase to purify the extract phase, and said extract phase is evaporated to yield a purified steroid hormone solid fraction.
Claim 2
Dependent Claim
102 words
The process of claim 1, wherein said fatty acids are separated from said ether phase by contacting said fatty acid extract with alcoholic KOH to saponify fatty acid esters therein, said saponified fatty acids are removed from the extract by contact with a water phase to purify the extract phase, said extract phase is evaporated to yield a purified steroid hormone solid fraction, fractionating said solid fraction in an activated alumina chromatographic column with an elutriant of 3 percent ether in cyclohexane to yield elutriant fractions containing separated steroid hormones, and evaporating elutriant from said fractions to produce separated steroid hormone solids.
Claim 3
Dependent Claim
66 words
The process of claim 1 wherein the fatty acids are separated from said ether phase by adsorbing the materials of said Fatty acid extract on a 100 percent activated alumina column, said column is washed with cyclohexane to remove impurities, and an elutriant agent of 3 percent ether in cyclohexane is passed through said column to chromatographically elute and separate steroid hormone fractions from said column.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-01-20 | 1978-07-22 | 1979-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-01-20 | 1982-07-22 | 1983-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-01-20 | 1986-07-22 | 1987-01-21 | $7700.00 | Window Closed / Lapsed |